LOCUS       RR217479                8190 bp    DNA     circular  SYN 
DEFINITION  Msh3 (myc-DDK-tagged) - Rat mutS homolog 3 (Msh3)
ACCESSION   NM_001191957
VERSION     NM_001191957.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4345..4436 
                     /label="Myc-DDK"  
     misc_feature    4466..5051 
                     /label="PolyA signal"  
     misc_feature    4523..4542 
                     /label="Restriction Enzyme"  
     misc_feature    5327..5999 
                     /label="ColE1"  
     misc_feature    6429..7223 
                     /label="Kan/Neo"  
     misc_feature    7258..7560 
                     /label="SV40 ori"  
     misc_feature    7646..8123 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4335..4343 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4335 
                     /label="Msh3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCAGG AAATCCGCGT CGGGAGGCTC TAATGCCGCC GGCCCAGACC 
     1081 CGGGGAGGCA AGTGGTTCTG AGCCGGTTCT TCAAGTCTGC GGGAAGCCTA AGATCCAGCG 
     1141 TGTCCCCCAC AGAACCAGCA GAGAAGGTGA CAGAAGGTGA CAGCAGGAAG AGGTCACTGG 
     1201 GGAATGGTGA GCCCACTAAC AAGAAAGCAA AGAAAGTTCC AGAGGAGGAA GGAGAAAATA 
     1261 CCTCAGTAGC ATCTCACAAT CCTGAGCCAA AGAAATGTCT GAGGCCCAGG ATTGTTTCAA 
     1321 AGTCCCTGGA AAAGTTGAAA GAATTCTGCT GTGATTCTGC CCTCCCTCAA AACAGAGTCC 
     1381 AGACAGAAGC CCTTTGGGAG AGACTTGCAG TTCTGCCAAA GTGTACTGAT TTCGAAGATA 
     1441 TCACTCTGCA ACGTGCAAAG AATGCGGTTT TGTCCGAAGA TTCCAAGTCT CAGGCTAATC 
     1501 AGAAAGACAC TCAATTTGGA CCTTCCCCTG AAGCTTTCCA GAAGACTTCC ACTTGTAAAC 
     1561 CTTTTAACAG GCGGTCCAAG AGCATCTATA CTCCACTAGA ACTGCAGTAC CTTGATGTGA 
     1621 AGCAGCTGCA TAAAGACGCG GTTTTGTGTG TGGAATGTGG GTACAAGTAC AGGTTCTTTG 
     1681 GGGAAGATGC AGAAATTGCT GCCCGGGAAC TCAATATTTA TTGCCACTTG GACCACAACT 
     1741 TTATGACGGC GAGCATACCC ACCCACAGAC TGTTCGTGCA TGTCCGCCGA CTGGTGGCCA 
     1801 AAGGATACAA GGTGGGAGTT GTGAAGCAAA CTGAAACTGC GGCATTAAAG GCCATTGGAG 
     1861 ACAATAAAAG TTCTGTCTTT TCCCGGAAAT TGACTGCTCT TTATACGAAA TCCACACTGA 
     1921 TTGGAGAAGA TGTGAATCCT CTGATCAGGC TGGATGATTC TGTAAATATT GATGAGGCAG 
     1981 TGACGGATAC TTCTACCAAC TATCTTCTGT GTATCTACGA AGAAAAGGAA AACATTAAAG 
     2041 ACAAAAAGAA GGGGAACATT TCTTTTGGCA TCGTGGGAGT GCAGCCTGCG ACGGGCGAGG 
     2101 TCGTGTTCGA CTGCTTCCAG GACTCGGCTT CCAGACTGGA GCTGGAGACT CGAACAGCAA 
     2161 GCCTGCAGCC CGTGGAGCTG CTGCTCCCCT CACAGCTGTC CGAGCCAACG GAGATGCTCA 
     2221 TCCGTAGAGC CACCGCTGTC AGTGTTGGGG ATGACAGAAT TCGAGTAGAA AGGATGAATA 
     2281 ATACTCACTT TGAATACAGC CATGCTTTCC AGACAGTTAT GGAGTTCTAT GCCAGAGAGA 
     2341 CAGTTGACAC CCAGGGCTCT CAGAGCCTGT CTGGTGTCAT TCACTTGGAG AAGCCTGTGA 
     2401 TCTGCGCCCT GGCTGCCATA ATACGGTACC TCAAGGAATT TAACTTGGAC AAGGTGCTGT 
     2461 CCAAACCCGA GAATTTTAAA CAGCTGTCAA GTGGAATGGA ATTCATGAGA ATCAATGGAA 
     2521 CAACGCTAAG GAATCTGGAA ATCCTACAGA ATCAGACTGA TATGAAGACC AGAGGAAGTT 
     2581 TGCTTTGGGT TTTAGACCAC ACTAAAACCT CATTTGGGAG AAGGAAGTTA AAAAAATGGG 
     2641 TTACCCAACC ATTGCTTAAA TTAAGGGACA TAAATGCCCG ACTTGATGCA ATATCCGATG 
     2701 TTCTCCACTC AGAATCCAGT GTGTTTGAAC AGATAGAAAA CCTTCTACGG AAACTACCGG 
     2761 ATGTCGAGAG AGGACTGTGT AGCATTTATC ACAAGAAGTG CTCTACTCAG GAGTTCTTCT 
     2821 TGATCGTCAA AAACCTGTGT CAACTCAAAT CAGAGCTTCA AGCGTTAATG CCTGCTGTTA 
     2881 ATTCCCACGT ACAGTCAGAC TTGCTCCGAG CACGCGTCTT AGAAGTGCCT GAGCTCCTCA 
     2941 GTCCAGTGGA GCCTTATTTA AAGGTTCTGA ATGAACAAGC TGCCAAAGCT GGCGATAAAA 
     3001 CTGAATTATT CAAAGACCTC TCTGACTTCC CTTTAATAAA AAAGAGGAAA AATGAAATTC 
     3061 AAGAAGTCAT TCACAGCATC CAAATGCATT TACAAGAATT GCGAAAAATA TTAAAACTTC 
     3121 CTTCTTTACA ATATGTGACT GTATCAGGAC AAGAGTTTAT GGTTGAAATA AAGAACTCAG 
     3181 CTGTGTCTTG CGTCCCGACT GACTGGGTGA AGGTTGGAAG CACAAAAGCT GTGAGCCGCT 
     3241 TTCACTCTCC TTTTGTCGTG GAAAACTACA GACGTCTGAA TCAACTCCGG GAGCAGCTAG 
     3301 TCCTTGACTG CAATGCTGAA TGGCTTGACT TTCTAGAGAA TTTTGGTGAA CATTACCACA 
     3361 CTTTGTGTAA GGCCGTGGAT CACCTAGCAA CTGTGGACTG CATTTTCTCC CTGGCCAAGG 
     3421 TCGCCAAGCA AGGAAGTTAC TGCAGGCCAA CTCTACAAGA AGAAAAGAAA ATCATCATAA 
     3481 AAAATGGAAG GCACCCTATG ATTGATGTGT TGCTGGGTGA ACAAGATCAG TTTGTGCCCA 
     3541 ACAGTACAAA TTTATCACAG GACTCTGAGA GAGTAATGAT AATCACCGGA CCGAACATGG 
     3601 GTGGCAAGAG CTCCTACATA AAACAGGTTG CTCTGGTTGT GATCATGGCT CAGATCGGCT 
     3661 CCTACGTTCC TGCAGAGGAA GCCACAATTG GGATTGTGGA CGGCATTTTC ACAAGGAGGG 
     3721 GTGCTGCAGA CAATATATAC AAAGGCCGGA GTACTTTCAT GGAAGAGCTG ACAGACACGG 
     3781 CGGAGATAAT CCAGAAAGCA ACACAGCGGT CCCTGGTTAT CCTGGACGAG CTGGGAAGAG 
     3841 GGACCAGCAC CCACGACGGA ATCGCCATTG CCTATGCGAC TCTAGAGTAT TTTATCAGAG 
     3901 ACGTGAAATC CTTAACCCTG TTCGTCACCC ACTATCCACC AGTCTGTGAA CTAGAGAAAC 
     3961 GTTACCCAGA GCAGGTGGGG AATTACCACA TGGGCTTCTT GGTCAATGAG GACGGAAGCA 
     4021 AGCAGGACTC AGGTGACATG GAGCAGATGC CGGACTCTGT CACTTTCCTC TATCAGATAA 
     4081 CCAGAGGAAT TGCAGCGAGG AGTTACGGAC TGAATGTGGC GAAGCTAGCA GATGTGCCCA 
     4141 GAGAAATCTT ACAGAAAGCT GCTCACAAGT CTAAAGAGCT GGAAGGCTTA GTCAATCTGA 
     4201 GAAGGAAAAG ACTTGAATAT TTTATAGATC TGTGGACGAC ACATAGTGTA AAGGACCTGC 
     4261 ACACGCGGGC AGCTGAGCTG GAAATACAGG AAATACAGAC GTCTCCCCAC AATGAAATGA 
     4321 AAACTACACT TTTGGAGCTG AAGACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4381 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4441 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4501 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4561 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4621 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4681 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4741 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4801 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4861 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4921 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4981 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5041 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5101 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5161 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5221 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5281 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5341 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5401 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5461 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5521 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5581 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5641 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5701 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5761 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5821 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5881 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5941 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6001 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6061 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6121 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6181 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6241 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6301 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6361 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6421 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6481 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6541 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6601 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6661 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6721 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6781 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6841 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6901 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6961 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7021 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7081 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7141 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7201 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7261 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7321 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7381 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7441 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7501 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7561 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7621 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7681 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7741 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7801 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7861 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7921 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7981 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8041 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8101 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8161 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//