LOCUS       RR209657                7653 bp    DNA     circular  SYN 
DEFINITION  Lpin1 (Myc-DDK-tagged ORF) - Rat lipin 1 (Lpin1), (10 ug)
ACCESSION   NM_001012111
VERSION     NM_001012111.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3810..3901 
                     /label="Myc-DDK"  
     misc_feature    3931..4516 
                     /label="PolyA signal"  
     misc_feature    3988..4007 
                     /label="Restriction Enzyme"  
     misc_feature    4792..5464 
                     /label="ColE1"  
     misc_feature    5894..6688 
                     /label="Kan/Neo"  
     misc_feature    6723..7025 
                     /label="SV40 ori"  
     misc_feature    7111..7588 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3792..3798 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3792 
                     /label="Lpin1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATTACGTG GGGCAGTTGG CCGGCCAGGT GTTTGTGACG GTGAAGGAGC 
     1081 TCTACAAGGG ACTGAACCCT GCCACGCTGT CAGGATGCAT CGACATCATT GTTGTCCGCC 
     1141 AGCCCAATGG CAGTCTGCAG TGCTCCCCGT TCCACGTCCG CTTCGGGAAG ATGGGTGTCC 
     1201 TACGCTCCCG AGAGAAAGTG GTGGACATAG AAATCAATGG GGAGTCTGTG GATTTGCACA 
     1261 TGAAGTTGGG AGACAACGGA GAAGCGTTTT TTGTTCAAGA GACTGACAAC GATCAGGAAG 
     1321 TCATCCCCAT GTACCTAGCC ACGTCCCCCA TCCTGTCAGA AGGAGCTTCG AGAATGGAAA 
     1381 GCCAGCTGAA AAGGAACTCT GTGGACAGAA TCCGGGGCCT GGATCCCACT ACAGCTGCCC 
     1441 AGGGCCTGCC TCCCAGCGAC ACCCCATCCA CTGGTTCTCT GGGGAAGAAG AGAAGGAAAA 
     1501 GGAGGAGGAA GGCCCATTTG GACAATCTCA AAAGAGATGA CAATGTCAAC ACATCTGAGG 
     1561 ACGAGGACAT GTTTCCCATA GAGATGAGCT CGGATGAGGA CACAGCACTG ATGGATGGGA 
     1621 GCAGAGCTCT TCCTAATGAT GTACCACCAT TCCAAGATGA TATTCCTAAG GAAAACCTCC 
     1681 CCTCGATTTC AACATATCCC CAGTCAGCAT CGTACCCCAA TTCAGACAGA GAGTGGTCCC 
     1741 CCAGCCCCAG CAGCCTGGTA GATTGTCAGA GGACTCCCCC TCACCTGGCC GAGGGAGTTC 
     1801 TCTCTAGCTC TTGTCCTCTG CAGTCTTGCC ACTTCCATGC TTCGGAAAGT CCTTCAGGCT 
     1861 CCCGGCCCTC AACACCAAAA AGTGATTCTG AGCTGGTCAG TAAGTCTGCA GACAGATTGA 
     1921 CGCCAAAGAA TAACCTGGAA ATGCTCTGGC TATGGGGTGA ATTGCCACAG GCTGCAAAGT 
     1981 CGTCTTCTCC ACACAAGATG AAAGACTCCA GCCCCTTAGG AGGTCGGAAG ATGGCTGATA 
     2041 AAATCCACTT CCAGACCATT CACAGCGAGT CTTCAGACAC TTTCAGTGAC CAGTCCCCAA 
     2101 CAATGGCCCG GGGGCTGCTC ATCCACCAGA GCAAGGCTCA GACGGAAATG CAGTTTGTGA 
     2161 ATGAGGAGGA CCTTGAGGCC TTGGGGGCCG CAGCCCCACC TTTGTCTGTA GCCGAAGAGC 
     2221 TCAAGGCCCC AGCCTCCAGC ACAGCACAGA CATCAAGCAA GTCAGATTCC CCTTCCAGAA 
     2281 AAAAAGATAA ACGAAGCCGG CACCTCGGAG CTGATGGCGT TTATCTGGAT GACCTCACAG 
     2341 ACATGGACCC GGAAGTGGCT GCCCTGTATT TCCCCAAGAA TGGGGATCCC GGTGGGCTTC 
     2401 CCAAACAAGC GAGTGACAAC GGAGCCAGGT CAGCCAACCA GTCCCCACAG TCGGTGGGTA 
     2461 GCTCGGGCAT TGACAGCGGC GTGGAGAGCA CCTCCGACAG CCTGAGGGAC CTGCCGTCCA 
     2521 TCGCCATCTC CCTCTGCGGT GGCCTCAGTG ACCACAGAGA GATCACCAAA GATGCATTTT 
     2581 TGGAACAAGC CGTGTCATAT CAGCAATTTG CCGACAACCC TGCTATCATC GATGATCCCA 
     2641 ACCTCGTGGT CAAGATTGGT AATAAGTACT ACAACTGGAC AACAGCAGCT CCTCTACTTC 
     2701 TGGCGATGCA GGCTTTCCAG AAACCTTTGC CAAAGGCCAC TGTGGAATCC ATCATGAGAG 
     2761 ATAAGATGCC CAAAAAGGGA GGGAGATGGT GGTTTTCCTG GAGAGGAAGA AACGCCACAA 
     2821 TCAAAGAGGA GAGCAAGCCT GAGCAGGGCC TGCCTGGGAA GGGCCACAAT ACCGGAGAGC 
     2881 AGCCTGCACA GCTTGGCCTG ACCACCAGAA TAAAGCATGA GTCATCCTCT AGTGATGAAG 
     2941 AGCACTCAGC TACCAAGCCA TCGAGCTCGA GCCACCTCTC TCTCTTGTCC AACGTCAGCT 
     3001 ACAAAAAGAC CCTGCGGCTC ACATCGGAGC AGTTGAAAAG CTTGAAGTTG AAGAATGGCC 
     3061 CCAACGATGT GGTGTTCAGT GTCACTACCC AGTACCAGGG CACCTGTCGC TGTGAGGGTA 
     3121 CCATCTACCT GTGGAATTGG GATGACAAAG TCATCATCTC AGATATCGAT GGTACCATCA 
     3181 CAAGATCTGA TACTCTTGGT CACATATTGC CCACACTGGG AAAGGATTGG ACTCACCAGG 
     3241 GCATCGCAAA GCTGTATCAC AAAGTAAGCC AGAATGGCTA CAAGTTTCTC TATTGTTCTG 
     3301 CACGTGCCAT TGGGATGGCA GACATGACGA GGGGCTATCT GCACTGGGTC AACGAGAGGG 
     3361 GCACAGTGCT GCCACAGGGG CCGCTTCTGC TGAGCCCCAG CAGCCTCTTC TCCGCCTTGC 
     3421 ACAGAGAAGT GATTGAAAAG AAGCCAGAAA AGTTCAAAGT CCAGTGTTTG ACAGACATCA 
     3481 AAAACCTGTT TTTCCCAAAC ACAGAGCCCT TTTATGCTGC TTTTGGGAAC CGGCCTGCCG 
     3541 ATGTGTATTC CTACAAGCAA GTGGGAGTGT CCCTGAATAG AATCTTCACC GTCAACCCCA 
     3601 AGGGTGAGCT GGTGCAGGAG CATGCCAAGA CCAACATCAG CTCGTATGTG CGGCTCTGCG 
     3661 AAGTGGTCGA CCACGTCTTC CCCTTGCTGA AGAGAAGCCA TTCCTGCGAC TTCCCCTGTT 
     3721 CAGACACTTT CAGTAACTTC ACCTTTTGGA GAGAGCCACT GCCACCCTTT GAAAACCAGG 
     3781 ACGTGCATTC AGCCTCAGCT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3841 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3901 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3961 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4021 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4081 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4141 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4201 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4261 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4321 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4381 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4441 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4501 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4561 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4621 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4681 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4741 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4801 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4861 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4921 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4981 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5041 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5101 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5161 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5221 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5281 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5341 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5401 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5461 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5521 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5581 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5641 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5701 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5761 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5821 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5881 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5941 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6001 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6061 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6121 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6181 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6241 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6301 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6361 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6421 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6481 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6541 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6601 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6661 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6721 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6781 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6841 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6901 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6961 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7021 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7081 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7141 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7201 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7261 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7321 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7381 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7441 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7501 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7561 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7621 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//