LOCUS       RR209156                7787 bp    DNA     circular  SYN 
DEFINITION  Ap2b1 (Myc-DDK-tagged ORF) - Rat adaptor-related protein complex 2, beta 1 subunit (Ap2b1), (10 ug)
ACCESSION   NM_080583
VERSION     NM_080583.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3944..4035 
                     /label="Myc-DDK"  
     misc_feature    4065..4650 
                     /label="PolyA signal"  
     misc_feature    4122..4141 
                     /label="Restriction Enzyme"  
     misc_feature    4926..5598 
                     /label="ColE1"  
     misc_feature    6028..6822 
                     /label="Kan/Neo"  
     misc_feature    6857..7159 
                     /label="SV40 ori"  
     misc_feature    7245..7722 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3926..3932 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3926 
                     /label="Ap2b1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGACTCC AAGTACTTCA CAACCAATAA GAAGGGAGAA ATCTTTGAAT 
     1081 TAAAAGCTGA ACTCAACAAT GAAAAGAAAG AAAAGAGGAA GGAGGCTGTG AAGAAAGTGA 
     1141 TTGCTGCTAT GACCGTGGGG AAGGATGTGA GCTCTCTCTT CCCAGATGTG GTGAACTGTA 
     1201 TGCAGACTGA CAACCTGGAA CTAAAGAAGC TTGTGTACCT CTATCTGATG AACTATGCCA 
     1261 AGAGTCAGCC AGACATGGCC ATCATGGCTG TCAACAGCTT TGTGAAGGAT TGTGAAGACC 
     1321 CCAATCCTTT GATTCGAGCC TTGGCAGTTA GAACCATGGG ATGCATCCGG GTGGACAAGA 
     1381 TTACAGAGTA TCTCTGTGAA CCCCTCCGCA AGTGCTTGAA GGATGAAGAC CCCTATGTTC 
     1441 GGAAAACAGC AGCAGTATGC GTGGCAAAAC TCCATGATAT CAATGCCCAG ATGGTGGAAG 
     1501 ATCAGGGATT TCTGGATTCT CTGCGGGATC TCATAGCAGA TTCAAACCCA ATGGTGGTGG 
     1561 CTAATGCTGT AGCAGCATTG TCTGAGATCA GTGAGTCTCA CCCAAACAGC AACTTACTTG 
     1621 ATCTGAACCC TCAGAATATC AATAAGCTGC TCACAGCCCT GAATGAGTGC ACTGAGTGGG 
     1681 GCCAGATTTT CATCTTGGAC TGCCTGTCTA ATTACAACCC TAAAGATGAC CGGGAAGCTC 
     1741 AGAGCATCTG TGAGCGAGTG ACGCCTCGGC TCTCTCATGC CAATTCTGCA GTGGTGCTTT 
     1801 CAGCAGTAAA AGTTCTGATG AAGTTTCTAG AGTTGTTACC CAAGGACTCT GACTACTACA 
     1861 ATATGCTGCT AAAGAAGCTA GCGCCTCCAC TTGTCACTTT GCTCTCTGGG GAGCCAGAAG 
     1921 TGCAGTATGT TGCCCTGAGG AACATCAACC TAATTGTCCA GAAAAGGCCT GAAATCTTGA 
     1981 AGCAGGAAAT CAAGGTCTTC TTTGTGAAGT ACAATGATCC TATCTATGTT AAACTAGAGA 
     2041 AGTTAGACAT CATGATTCGT CTTGCATCCC AAGCCAACAT TGCTCAGGTT CTGGCAGAAC 
     2101 TGAAGGAATA TGCCACTGAA GTTGATGTGG ACTTTGTTCG CAAAGCTGTG AGGGCCATTG 
     2161 GACGGTGTGC CATCAAAGTG GAGCAATCAG CAGAACGCTG TGTGAGCACA CTGCTTGATC 
     2221 TAATCCAGAC CAAAGTAAAT TATGTGGTCC AAGAGGCAAT TGTTGTCATC AGGGACATCT 
     2281 TCCGAAAATA CCCCAACAAG TATGAGAGCA TTATCGCCAC GCTGTGTGAG AACTTGGACT 
     2341 CCCTGGATGA ACCCGATGCC CGAGCGGCTA TGATTTGGAT TGTAGGAGAG TATGCTGAAA 
     2401 GAATCGATAA TGCCGATGAG TTACTAGAGA GCTTCCTGGA AGGTTTTCAT GATGAAAGCA 
     2461 CCCAGGTGCA GCTCACGTTG CTTACCGCCA TAGTGAAACT GTTTCTCAAG AAGCCATCAG 
     2521 AAACACAGGA GCTGGTCCAA CAGGTCTTGA GCTTGGCCAC ACAGGATTCT GATAATCCTG 
     2581 ACCTTCGAGA TCGGGGTTAT ATTTATTGGC GCCTTCTTTC AACTGACCCT GTGACAGCCA 
     2641 AAGAAGTAGT GTTGTCTGAG AAGCCATTGA TCTCTGAGGA AACAGACCTC ATTGAACCTA 
     2701 CCCTCCTGGA TGAGCTCATC TGCCACATTG GTTCTTTGGC CTCCGTGTAC CATAAACCTC 
     2761 CGAATGCTTT TGTGGAAGGG AGCCATGGCA TTCATCGCAA ACACTTGCCA ATTCACCATG 
     2821 GGAGCACTGA TGCAGGTGAT AGCCCTGTTG GCACCACCAC TGCAACCAAC CTGGAACAGC 
     2881 CTCAGGTCAT CCCCTCTCAA GGTGACCTTC TGGGGGATCT TTTAAATCTT GACCTGGGTC 
     2941 CCCCAGTGAA TGTCCCGCAA GTGTCCTCCA TGCAGATGGG AGCAGTGGAT CTTTTAGGAG 
     3001 GAGGACTGGA TAGCCTGCTT GGCAGTGACC TTGGCGGGGG CATTGGAGGA AGTCCGGCAG 
     3061 TAGGACAGTC CTTCATCCCG TCATCAGTGC CTGCAACCTT CGCTCCTTCA CCTACTCCTG 
     3121 CTGTGGTCAG CAGTGGTCTG AATGACCTGT TTGAGCTTTC CACTGGGATA GGCATGGCAC 
     3181 CTGGCGGATA TGTGGCTCCT AAGGCAGTCT GGCTACCTGC TGTAAAGGCT AAAGGCTTGG 
     3241 AGATTTCGGG GACGTTTACT CACCGCCAAG GGCACATCTA TATGGAAATG AACTTCACCA 
     3301 ACAAAGCTCT GCAGCACATG ACGGATTTTG CCATCCAGTT TAACAAGAAT AGCTTCGGTG 
     3361 TCATCCCGAG CACTCCCTTG GCCATACATA CTCCGCTGAT GCCAAACCAG AGCATTGATG 
     3421 TGTCTCTGCC TCTCAACACC TTGGGCCCAG TCATGAAGAT GGAGCCTCTG AATAACTTGC 
     3481 AGGTGGCTGT TAAAAACAAT ATTGATGTCT TCTACTTCAG CTGCCTCATC CCACTCAATG 
     3541 TGCTTTTTGT AGAAGATGGC AAAATGGAAC GCCAGGTCTT CCTTGCGACG TGGAAGGATA 
     3601 TTCCCAATGA AAATGAGCTC CAATTTCAGA TTAAGGAGTG TCATTTAAAC GCTGACACAG 
     3661 TTTCCAGCAA GTTGCAAAAC AACAATGTTT ACACTATCGC CAAGAGGAAT GTGGAGGGGC 
     3721 AGGACATGCT GTACCAGTCC CTGAAGCTCA CTAATGGCAT TTGGATTTTG GCAGAGCTGC 
     3781 GGATCCAGCC AGGAAACCCC AATTATACGC TGTCGCTGAA GTGTAGAGCC CCTGAAGTCT 
     3841 CTCAGTACAT CTATCAGGTC TACGACAGCA TTTTGAAAAA CCCCCTGAAG TCTCTCAGTA 
     3901 CATCTATCAG GTCTACGACA GCATTTTGAA AAACACGCGT ACGCGGCCGC TCGAGCAGAA 
     3961 ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA 
     4021 TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC 
     4081 CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA 
     4141 GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA 
     4201 TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG 
     4261 CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA 
     4321 TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC 
     4381 AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA 
     4441 TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA 
     4501 AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT 
     4561 AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG 
     4621 TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA 
     4681 TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 
     4741 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 
     4801 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 
     4861 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 
     4921 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 
     4981 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 
     5041 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 
     5101 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 
     5161 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 
     5221 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 
     5281 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 
     5341 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 
     5401 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 
     5461 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 
     5521 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 
     5581 GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT 
     5641 TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA 
     5701 TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA 
     5761 ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT 
     5821 CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC 
     5881 TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG 
     5941 AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC 
     6001 GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC 
     6061 GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT 
     6121 CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT 
     6181 AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA 
     6241 CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA 
     6301 TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA 
     6361 GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT 
     6421 TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT 
     6481 CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG 
     6541 GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG 
     6601 CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT 
     6661 TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC 
     6721 GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC 
     6781 TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG 
     6841 ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT 
     6901 CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA 
     6961 ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC 
     7021 GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG 
     7081 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT 
     7141 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC 
     7201 CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     7261 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     7321 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     7381 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     7441 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     7501 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     7561 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     7621 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     7681 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     7741 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//