LOCUS       RR209092                7077 bp    DNA     circular  SYN 
DEFINITION  Fbxo34 (Myc-DDK-tagged ORF) - Rat F-box protein 34 (Fbxo34), (10 ug)
ACCESSION   NM_001107257
VERSION     NM_001107257.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3234..3325 
                     /label="Myc-DDK"  
     misc_feature    3355..3940 
                     /label="PolyA signal"  
     misc_feature    3412..3431 
                     /label="Restriction Enzyme"  
     misc_feature    4216..4888 
                     /label="ColE1"  
     misc_feature    5318..6112 
                     /label="Kan/Neo"  
     misc_feature    6147..6449 
                     /label="SV40 ori"  
     misc_feature    6535..7012 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3216..3222 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3216 
                     /label="Fbxo34"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTTTCGGG GCGAGCGTTG GGCTCCGCCG CCCAGCGCTG AGCTCCTGGT 
     1081 CCGTCTCAGT CTCGGGGAGC GAGTCAGGCC TGCCGCCGCC TCATCGGGCT GCCGCAGCCC 
     1141 CGGGCCAGGC CCCGGCCGGG CAGACGCACA GGGCATCTGT TATGCACCTT AAGCCATACT 
     1201 GGAAGCTCCA GAAGAAAGAG CGGCCTCTGA GCCACGGAAA CACGGACGGT GATGCGAAGG 
     1261 GCAGAGCTAA CTGCATGAAA CCCACCCCCT CCCCTTCCGC CTCTCCTGTC AAAGCATCAT 
     1321 CTAGGAAGCC TTTCGGGATC CTTTCTCCAA ATGTCCTGTG CAGCATGAGT GGGAAGAGCC 
     1381 CCGTGGAGAA CAGCTTGAGT GTTAAAGCCA CAAGGAATGC GCCGTCTGCA GCCGTGCAGC 
     1441 AGACTGAAGG AGGCCTGCCG GGGAGCTGGG CTATTGTCAA ACCTGGAAAC ACCAAGGAGA 
     1501 AAATTGCGTT CTTTGCAGCC CACCAGTGCA GCAATAGGAT AGGCTCTATG AAAATAAAAA 
     1561 GCTCCTGGGA TATCGATGGG AGAGCCACTA AACGAAGGAA AAAATCAGGG GATCTTAAGA 
     1621 AAGCCAAGTT ACAGCTAGAA ATGATGAGGG AGATCAACAG CCAGTGCTAC CAGTCGGAGC 
     1681 CATTTGCCTG TGGTGTCGAG CACTGTTCTG TGCATTACAT GAGTGACAGT GGGGACGGTG 
     1741 TCTATGCTGC GAGGCCTCTG TCAGTGATAC AGATGGTTGC CTTCCTCGAG CAGAGAGCCA 
     1801 CTGCTCTGCT AGCTAGCTGC ACAAAAAACT GCACAAATTC GCCTGCCATT GTGAAGATTT 
     1861 CTGGCCAGTC CCGAGGTATG CCACCTGTAC CTGAGCCCTT CTCTGCTCCA GACACTTGTG 
     1921 AAGAATCCAA GGAAAGAGAA AATCCTGAGA TTGGCAGATC CCAGAGTGAG CCAGTCCGTG 
     1981 TCCTTGACAT GGTAGCCAGG TTGGAGTCCG AGTGCCTGAA GCACCAGGGC CAGCGGGAAC 
     2041 CTGGGAGCTT GTCACGGAAT AACAGCTTCC GTCGGAATGT AGGCAGGGTG TTGCTCACAA 
     2101 ACGGCTCTCA GGCCAGCGAC AAAAACGGAG AGGGCTCTGC AGACACGCCC GGCACTCAGG 
     2161 TGCACCCCTT GCAGCTGGTA TCTGTGGGTG AAGGACCCCC TGCAACTGAC CGTTCTGCAG 
     2221 GGGAGCAGGC CTGGGATGGC ACTTCTCAGG GCTGTCCCTC ATTGCCGGCT GATGTGAGCT 
     2281 TCCACACAGA CAGCACCGAA TCAGAGCCGG CTCTGCATAC AGCTGTGAAA CACTGCAGCC 
     2341 GCAATGATGT AGAAATGGCA GAGGAATTTG ATGAATTGCC TACAGATGCT GTCAGCTGTA 
     2401 GAGAGCTTGT AACACTCACA AGCCACAGTC CTGAGCAGAG ACAAGAACCT CTGTGCGTTA 
     2461 GCATCACTGT GTGCACAGTG GAGAAAGCCC AGCCATCTGC GCTAGACCCC CTTGAAGAGC 
     2521 CTCTTCCTGG GATGCTGTTC TTTCTGTCAC CTGGGCAGGA CCAGCAGGCG CACCCCCAGT 
     2581 TGAGTGAACA CCCAGCACCA GAGGCGACAG AGGCCAGTCA GCCTCCAGAT GCTGCTGAGG 
     2641 GCAGCAGTGC CCGTGAGGAG AAAGATGCCT CTCTGGAGCC GCTTGTTCCA GCAGCCTCTG 
     2701 GGGAAGGAAG CACCTCACAG GGGCTTGAGG CAGCCACGTG TAAGAAGCAG GTGTCTCAGG 
     2761 ACTTCCTGGA GACCAGGTTT AAAATCCAGC AACTTCTGGA ACCTCAGCAG TACATGGCAT 
     2821 GCCTGCCTCA CCACATCATG GTGAAAATCT TCAGGTTACT CCCGACCCTG AGCTTAGCAA 
     2881 TCCTTAAATG TACCTGCCGC TACTTCAAGT CCATCATTGA ATACTACAAC ATCAGGCCAG 
     2941 CGGACTCTCG GTGGGTCCGA GACCCGCGCT ATAGAGAAGA CCCTTGCAAG CAGTGCAAGA 
     3001 AAAAGTATGT GAAAGGGGAC GTGTCCCTGT GCCGGTGGCA CCCCAAACCC TACTGCCAGG 
     3061 CATTGCCGTA TGGGCCTGGG TACTGGATGT GCTGCCACCA GTCTCAGAAG GGCTTTCCGG 
     3121 GCTGTAAGTT AGGCCTTCAT GACAATCACT GGTTACCCGC CTGCCACAGC TTTAATCGGG 
     3181 CAATCCATAA GAAATCAAGA GGGAGTGAAA CGGAAGAAGA GTACACGCGT ACGCGGCCGC 
     3241 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3301 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3361 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3421 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3481 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3541 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3601 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3661 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3721 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3781 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3841 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3901 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3961 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4021 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4081 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4141 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4201 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4261 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4321 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4381 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4441 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4501 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4561 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4621 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4681 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4741 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4801 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4861 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4921 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4981 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5041 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5101 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5161 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5221 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5281 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5341 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5401 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5461 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5521 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5581 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5641 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5701 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5761 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5821 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5881 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5941 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6001 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6061 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6121 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6181 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6241 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6301 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6361 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6421 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6481 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6541 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6601 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6661 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6721 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6781 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6841 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6901 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6961 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7021 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//