LOCUS       RR208672                7908 bp    DNA     circular  SYN 
DEFINITION  Myo1d (Myc-DDK-tagged ORF) - Rat myosin ID (Myo1d), (10 ug)
ACCESSION   NM_012983
VERSION     NM_012983.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4063..4154 
                     /label="Myc-DDK"  
     misc_feature    4184..4769 
                     /label="PolyA signal"  
     misc_feature    4241..4260 
                     /label="Restriction Enzyme"  
     misc_feature    5045..5717 
                     /label="ColE1"  
     misc_feature    6147..6941 
                     /label="Kan/Neo"  
     misc_feature    6976..7278 
                     /label="SV40 ori"  
     misc_feature    7364..7841 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4038..4045 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4038 
                     /label="Myo1d"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGCAG GAGAGCCTTG AGTTTGGCAA GGCGGACTTC GTGCTGATGG 
     1081 ACACCGTCTC CATGCCCGAG TTCATGGCCA ACCTTAGGCT CAGATTTGAG AAAGGACGCA 
     1141 TCTATACATT CATTGGCGAG GTGGTCGTTT CCGTGAACCC ATACAAGGTG CTGAACATCT 
     1201 ATGGAAGGGA CACAATTGAG CAGTACAAAG GTCGGGAGCT GTACGAGAGA CCTCCTCATC 
     1261 TCTTCGCTAT TGCTGACGCT GCTTACAAGG CTATGAAGAG GCGATCAAAA GACACCTGTA 
     1321 TTATGATATC AGGGGAAAGT GGAGCTGGTA AAACAGAGGC CAGTAAGTAC ATCATGCAGT 
     1381 ATATCGCCGC CATCACCAAC CCCAGCCAGA GAGCAGAGAT AGAAAGAGTG AAGAATATGT 
     1441 TGCTGAAGTC AAACTGTGTT TTGGAAGCTT TTGGAAATGC CAAAACCAAT CGCAATGACA 
     1501 ATTCCAGCAG GTTTGGGAAA TACATGGATA TCAACTTTGA CTTCAAGGGA GACCCCATTG 
     1561 GGGGACATAT TAATAACTAC TTGCTGGAAA AGTCTCGTGT AATTGTGCAA CAGCCCGGAG 
     1621 AACGAAGCTT CCATTCTTTC TATCAGCTAC TTCAGGGAGG TTCCGAGCAG ATGCTGCATT 
     1681 CTCTACATCT GCAGAAATCA CTCTCATCCT ACAACTACAT CCGTGTGGGG GCACAACTGA 
     1741 AGTCTTCCAT CAATGATGCT GCAGAGTTCA AAGTTGTAGC TGATGCCATG AAAGTAATTG 
     1801 GATTCAAACC TGAGGAGATT CAAACAGTGT ATAAAATTCT GGCTGCTATT CTTCACTTGG 
     1861 GAAATTTAAA ATTTATAGTA GATGGTGACA CGCCACTTAT TGAGAATGGC AAAGTTGTAT 
     1921 CTGTCATAGC AGAATTGCTC TCAACCAAGG CAGACATGGT GGAGAAAGCC CTGCTTTACC 
     1981 GGACGGTGGC CACAGGCCGT GACATCATTG ACAAACAGCA CACGGAACAG GAGGCTAGCT 
     2041 ACGGCAGAGA CGCCTTTGCC AAGGCAATAT ATGAGCGCCT CTTTTGTTGG ATTGTTACTC 
     2101 GCATCAATGA CATCATCGAG GTCAAGAACT ACGACACCAC AGTACATGGG AAAAACACAG 
     2161 TTATTGGCGT CTTGGATATC TATGGCTTTG AAATCTTTGA CAACAACAGC TTCGAGCAGT 
     2221 TCTGCATTAA CTACTGCAAT GAGAAGCTGC AGCAGCTGTT CATTCAGCTG GTGCTGAAGC 
     2281 AGGAGCAAGA GGAGTACCAG CGGGAAGGAA TCCCCTGGAA ACACATTGAT TACTTCAACA 
     2341 ACCAGATCAT TGTGGACCTC GTGGAGCAGC AGCACAAAGG CATCATTGCA ATCCTGGATG 
     2401 ACGCGTGTAT GAATGTTGGC AAAGTCACTG ATGGAATGTT CCTCGAGGCC CTTAACAGTA 
     2461 AATTGGGCAA ACATGGTCAT TTTTCCAGCC GAAAGACCTG TGCCTCAGAC AAAATCCTGG 
     2521 AGTTTGATCG GGACTTTCGA ATCCGGCATT ATGCAGGTGA TGTCGTGTAT TCTGTCATCG 
     2581 GTTTCATTGA CAAAAACAAA GATACTTTAT TTCAAGACTT CAAGCGCCTC ATGTATAACA 
     2641 GCTCAAATCC TGTGCTGAAG AACATGTGGC CGGAAGGCAA ACTGAGCATT ACAGAGGTGA 
     2701 CCAAGAGGCC TCTGACCGCC GCCACCCTGT TTAAGAATTC CATGATTGCT TTAGTAGACA 
     2761 ACCTTGCTTC AAAGGAGCCA TACTATGTAC GTTGCATCAA ACCCAACGAC AAGAAGTCCC 
     2821 CACAGATTTT TGATGATGAG CGCTGCCGCC ATCAGGTAGA ATACCTGGGA CTCCTGGAAA 
     2881 ATGTGAGAGT GCGCCGGGCA GGGTTTGCCT TCCGCCAGAC CTATGAGAAG TTTCTTCACA 
     2941 GATACAAGAT GATCTCTGAG TTCACCTGGC CTAACCACGA CCTTCCTTCA GACAAGGAGG 
     3001 CTGTCAAGAA ACTCATAGAG CGATGTGGCT TTCAGGATGA CGTAGCATAT GGGAAGACCA 
     3061 AAATTTTCAT TCGAACACCC CGTACGCTGT TCACTTTGGA AGAACTCCGC GCCCAGATGC 
     3121 TCGTCAGGGT TGTCCTCTTC CTACAAAAGG TGTGGCGTGG CACCCTGGCC CGCATGCGGT 
     3181 ACAAGAGGAC CAAGGCAGCT CTGACAATAA TCAGGTACTA CCGGCGCTAT AAAGTGAAGT 
     3241 CGTACATCCA CGAGGTAGCC AGGCGATTCC ACGGGGTCAA GAACATGAGA GACTACGGGA 
     3301 AGCACGTGAA GTGGCCAACC CCACCCAAAG TCCTGCGGCG TTTTGAGGAG GCCCTGCAGT 
     3361 CGATTTTTAA TAGATGGAGA GCATCCCAAC TCATCAAGAC CATACCTGCA TCAGACCTTC 
     3421 CCCAGGTCAG GGCAAAGGTT GCAGCCATGG AGATGTTGAA AGGTCAAAGG GCAGACCTTG 
     3481 GGCTCCAGAG GGCCTGGGAA GGCAACTATC TTGCTTCAAA GCCAGATACA CCTCAGACCT 
     3541 CGGGCACTTT CGTCCCAGTT GCTAATGAGC TGAAACGCAA GGACAAATAT ATGAATGTTC 
     3601 TCTTTTCCTG TCATGTCCGA AAGGTGAATC GATTTAGTAA GGTAGAAGAC CGAGCAATTT 
     3661 TTGTCACTGA CCGTCACCTG TATAAAATGG ACCCCACTAA GCAGTACAAG GTGATGAAGA 
     3721 CCATCCCACT GTACAATTTG ACTGGCCTGA GTGTGTCCAA TGGAAAGGAC CAGCTTGTAG 
     3781 TGTTCCACAC AAAAGACAAC AAAGACCTCA TCGTCTGCCT CTTCAGCAAA CAGCCGACCC 
     3841 ATGAGAGCCG GATTGGAGAA CTTGTTGGAG TCCTGGTGAA TCATTTCAAG AGTGAGAAGC 
     3901 GCCACCTTCA AGTGAACGTC ACCAACCCGG TGCAGTGCAG CCTGCATGGG AAGAAATGCA 
     3961 CCGTATCCGT GGAGACCCGG CTTAACCAGC CACAGCCTGA CTTCACCAAG AACCGCTCCG 
     4021 GCTTCATCCT CAGTGTGCCC GGGAATAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     4081 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4141 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4201 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4261 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4321 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4381 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4441 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4501 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4561 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4621 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4681 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4741 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4801 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4861 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4921 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4981 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5041 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5101 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5161 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5221 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5281 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5341 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5401 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5461 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5521 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5581 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5641 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5701 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5761 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5821 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5881 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5941 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6001 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6061 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6121 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6181 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6241 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6301 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6361 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6421 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6481 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6541 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6601 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6661 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6721 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6781 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6841 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6901 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6961 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7021 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7081 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7141 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7261 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7321 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7381 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7441 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7501 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7561 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7621 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7681 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7741 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7801 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7861 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//