LOCUS       RR207695L3                8834 bp    DNA     circular  SYN 
DEFINITION  Cyfip2 (Myc-DDK-tagged ORF) - Rat cytoplasmic FMR1 interacting protein 2 (Cyfip2), (10 ug)
ACCESSION   NM_001106996
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7125..7305 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8033..8692 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7353..7972 
                     /label="ColE1"  
     misc_feature    5165..5188 
                     /label="DDK"  
     misc_feature    6000..6020 
                     /label="LR50"  
     misc_feature    5117..5188 
                     /label="MYC"  
     misc_feature    5198..5263 
                     /label="P2A"  
     misc_feature    5264..5863 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6009..6600 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5099..5105 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5099 
                     /label="Cyfip2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCCTCTACC CCTTGGATCT GTACAACGAC 
     3361 AGCGCCTACT ATGCGCTCAC CAAGTTCAAA AAGCAGTTCC TGTATGACGA GATCGAAGCA 
     3421 GAGGCAAGTG AACCTGTGCT TTGATCAGTT TGTCTACAAG CTGGCTGACC AGATCTTTGC 
     3481 CTACTACAAA GCCATGGCCG GCAGTGTCCT GTTGGACAAA CGTTTTAGAG CTGAATGTAA 
     3541 GAACTATGGA GTGATCATTC CGTACCCGCC ATCTAACCGG TACGAGACAC TGCTGAAACA 
     3601 GAGACACGTC CAGCTGTTGG GTAGATCAAT TGACTTGAAC AGACTCATCA CGCAGCGGAT 
     3661 CTCTGCCGCC ATGTACAAAT CCTTGGACCA GGCAATCAGC CGCTTTGAGA GCGAAGACCT 
     3721 GACCTCCATT GTGGAGCTAG AGTGGCTTCT GGAGATTAAC CGGCTCACAC ACCGACTCCT 
     3781 CTGTAAGCAC ATGACGCTAG ACAGCTTCGA CGCCATGTTC CGAGAAGCCA ATCATAATGT 
     3841 GTCTGCCCCC TATGGCCGCA TCACCCTGCA TGTCTTCTGG GAGCTGAACT TTGACTTCCT 
     3901 CCCCAACTAC TGCTATAACG GCTCCACCAA CCGCTTTGTC CGCACTGCCA TCCCTTTCAC 
     3961 CCAAGAACCA CAACGGGATA AGCCCGCCAA TGTCCAGCCT TACTACCTCT ACGGATCCAA 
     4021 GCCTCTCAAC ATTGCCTACA GCCACATCTA CAGCTCCTAC CGCAACTTTG TGGGGCCCCC 
     4081 TCACTTCAAG ACCATCTGCA GACTGCTGGG CTACCAGGGC ATCGCCGTGG TCATGGAGGA 
     4141 ACTGCTGAAA ATCGTCAAGA GCTTGCTCCA GGGTACCATT CTGCAGTACG TGAAGACACT 
     4201 CATAGAGGTG ATGCCTAAAA TATGCCGTTT GCCTCGTCAC GAGTATGGCT CTCCAGGGAT 
     4261 CCTGGAGTTC TTCCACCACC AACTGAAGGA CATCATCGAG TATGCAGAAC TCAAAACAGA 
     4321 CGTGTTCCAG AGTCTCAGAG AGGTGGGCAA TGCCATCCTG TTCTGCCTCC TCATAGAGCA 
     4381 AGCTCTGTCT CAAGAGGAAG TCTGTGATTT GCTCCATGCC GCACCCTTCC AAAACATTCT 
     4441 GCCTCGGGTC TATATCAAAG AGGGGGAGCG TCTGGAAGTC CGGATGAAAC GCCTCGAGGC 
     4501 CAAGTACGCC CCACTTCACC TGGTCCCCCT GATAGAGCGA CTGGGGACGC CTCAGCAAAT 
     4561 TGCCATCGCT CGGGAGGGTG ACCTGCTGAC CAAGGAGCGG CTCTGCTGTG GCCTGTCCAT 
     4621 GTTCGAGGTT ATCCTGACCC GAATTCGGAG CTACCTGCAG GACCCCATCT GGAGGGGCCC 
     4681 GCCACCCACC AATGGCGTCA TGCACGTGGA TGAGTGTGTT GAGTTTCACC GGCTGTGGAG 
     4741 CGCCATGCAG TTTGTCTACT GCATCCCTGT GGGGACCAAC GAGTTCACAG CTGAGCAGTG 
     4801 CTTCGGTGAT GGCTTGAACT GGGCTGGCTG CTCTATCGTT GTCTTGCTGG GCCAGCAACG 
     4861 GCGCTTTGAC CTGTTTGATT TCTGTTATCA TCTGCTGAAG GTGCAGAGGC AGGACGGGAA 
     4921 GGATGAGATC ATTAAGAATG TGCCCCTGAA GAAGATGGCT GACCGCATCA GGAAGTACCA 
     4981 GATCTTGAAC AATGAGGTTT TTGCCATTCT GAACAAGTAC ATGAAGTCCG TAGAGACAGA 
     5041 CAGCTCCACG GTAGAGCACG TACGCTGCTT CCAGCCCCCA ATCCACCAGT CCCTGGCCAC 
     5101 CACCTGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC 
     5161 AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TGGGTAGGAA GCGGAGCTAC 
     5221 TAACTTCAGC CTGCTGAAGC AGGCTGGAGA CGTGGAGGAG AACCCTGGAC CTATGACCGA 
     5281 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT 
     5341 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ATCGCCACAT 
     5401 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA 
     5461 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA 
     5521 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT 
     5581 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG 
     5641 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT 
     5701 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC 
     5761 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA 
     5821 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GATGTACACC 
     5881 CAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT AACTTCGTAT 
     5941 AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG GTACAATTCG 
     6001 ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA 
     6061 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC 
     6121 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT 
     6181 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG 
     6241 CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT 
     6301 TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT 
     6361 GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT 
     6421 CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT 
     6481 ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC 
     6541 GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT 
     6601 CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC AATCACAAGT 
     6661 AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG 
     6721 GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA 
     6781 GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA 
     6841 AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG 
     6901 AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA 
     6961 GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA 
     7021 CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT 
     7081 GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTCT 
     7141 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT 
     7201 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC 
     7261 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGCATG 
     7321 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 
     7381 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 
     7441 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 
     7501 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG 
     7561 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 
     7621 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT 
     7681 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 
     7741 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 
     7801 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC 
     7861 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 
     7921 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC 
     7981 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 
     8041 ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA 
     8101 CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT 
     8161 CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG 
     8221 CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT 
     8281 TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG 
     8341 AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG 
     8401 TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT 
     8461 CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT 
     8521 GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA 
     8581 TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT 
     8641 GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA 
     8701 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8761 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8821 AAGTGCCACC TGAC
//