LOCUS       RR206550                6585 bp    DNA     circular  SYN 
DEFINITION  Otop3 (Myc-DDK-tagged ORF) - Rat otopetrin 3 (Otop3), (10 ug)
ACCESSION   NM_001105852
VERSION     NM_001105852.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2742..2833 
                     /label="Myc-DDK"  
     misc_feature    2863..3448 
                     /label="PolyA signal"  
     misc_feature    2920..2939 
                     /label="Restriction Enzyme"  
     misc_feature    3724..4396 
                     /label="ColE1"  
     misc_feature    4826..5620 
                     /label="Kan/Neo"  
     misc_feature    5655..5957 
                     /label="SV40 ori"  
     misc_feature    6043..6520 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2724..2730 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2724 
                     /label="Otop3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCGCAG ACTTCAGAAG CAGAGACCAC TGAGGCAGCA TCTACCTATG 
     1081 ACCAAGCAAA CATGGACGTG GAGACCAAGC ACACAGGGCC CCCAGGCGCC CCAAAGCAGA 
     1141 AGTCCTGGCT GGCTCTGCAT TTCTCCCTGC TGCTGCGGAG AGACCGGCAA GCCCAGAAGG 
     1201 CGGGGCAGCT CTTCTCGGGG CTCCTGGCTC TCAATGTGGT ATTCCTGGGC GGGGCTTTTA 
     1261 TCTGCAGCAT GATCTTCAAC AAGGTGGCTG TCACTCTGGG GGACGTGTGG ATCCTGCTGG 
     1321 CCGCACTGAA GGTCCTCTCT CTGTTGTGGC TCCTCTACTA CACCGTGGGT ACCACACGGA 
     1381 AGCCCCACGC AGTGCTGTAC CGCGATCCTC ACGCGGGACC GGTTTGGGTG AGGGGCTCCC 
     1441 TGGTGCTCTT CGGCAGCTGT ACCGTCTGCC TGAATGTATT TCGGATGGGC TACGACGTGA 
     1501 GCCACATTCA CTGCAAGTCA GAAGTGGAGC TTATCTTTCC AGCCATTGAG ATCGTCTTTA 
     1561 TGATCATCCA GACCTGGGTG CTCTGGAAAC ACTGTAAGGA CTGTGTTCAG GTGCAGACCA 
     1621 ACTTCACCAG GTGTGGGCTC ATGCTGACGC TGGCCACAAA CCTGCTGATG TGGGTTTTGG 
     1681 CTGTGACCAA TGACTCCATG CATCGTGAGA TTGAGGCTGA GCTCGATGCC CTCATGGAAA 
     1741 AGTTCTCAGG TAACGGGACC AACACTTGCA TGTGCCTCAA CGCCACCGTG TGCGAGGTCT 
     1801 TCCGGAAGGG CTTCCTGATG CTCTACCCTT TCAGCACTGA GTACTGCCTC ATCTGCTGTG 
     1861 CTGTGCTCTT CGTCATGTGG AAGAACGTGG GCCGCAGCCT GGCAGCCCAC TCAGGTGCCC 
     1921 ATCCCAACAG ACCACCCTTC CGCCTGCACG GGGCCATCTT TGGGCCACTG CTGGGCCTTT 
     1981 TGGCACTGGT GGCTGGGGTA TGCGTCTTTG TGCTCTTCCA GATCGAAGCG AGTGGCCCTG 
     2041 ACATAGCCCG CCAGTACTTC ACCCTGTACT ATGCATTCTA TGTGGCTGTG CTGCCCACCA 
     2101 TGAGCCTGGC GTGCCTGGCG GGCACAGCCA TCCATGGACT GGAGGAGCGT GAACTGGACA 
     2161 CGCTCAAAAA CCCCACACGC AGCCTGGATG TGGTGCTGCT GATGGGCGCT GCACTGGGCC 
     2221 AGATGGGCAT TGCCTACTTC TCCATCGTGG CTATCGTGGC CACCCAGCCA CATCAGCTAC 
     2281 TCAACCAGCT CATCCTGGCC TACTCACTGC TGCTTATCCT GCAGCACATC ACTCAGAATC 
     2341 TCTTCATCAT CGAGGGCCTG CACCGCCGTC CTCTCTGGGA GCCTGTGATC TCTGGTGTGC 
     2401 CGGAGAAGCA GGATATTGAG CTACCCCGCA GAGGCTCCCT GCGGGAGCTG GGTCAGGACC 
     2461 TACGGAGAGC CTCAAGGGCC TACATCCACT CCTTCAGCCA CCTCAACTGG AAACGCAGGA 
     2521 TGCTCAAGGA GATCTCTCTC TTCCTCATCC TCTGCAATAT CACACTGTGG ATGATGCCTG 
     2581 CCTTTGGCAT ACACCCAGAG TTTGAGAATG GACTGGAAAA GGATTTTTAT GGCTACAGGA 
     2641 CCTGGTTCAC CATTGTTAAC TTCGGCCTGC CTCTGGGGGT CTTCTACCGC ATGCACTCTG 
     2701 TTGGGGGACT CGTGGAGGTC TACCTGGGGG CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     2761 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     2821 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     2881 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     2941 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3001 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3061 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3121 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3181 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3241 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3301 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3361 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3421 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3481 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     3541 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     3601 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     3661 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     3721 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     3781 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     3841 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     3901 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     3961 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4021 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4081 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4141 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4201 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4261 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4321 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4381 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4441 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4501 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     4561 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     4621 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     4681 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     4741 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     4801 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     4861 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     4921 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     4981 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5041 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5101 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5161 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5221 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5281 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5341 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5401 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5461 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     5521 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     5581 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     5641 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     5701 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     5761 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     5821 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     5881 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     5941 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6001 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6061 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6121 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6181 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6241 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6301 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6361 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6421 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6481 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     6541 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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