LOCUS       RR202950                8007 bp    DNA     circular  SYN 
DEFINITION  Skiv2l2 (Myc-DDK-tagged ORF) - Rat superkiller viralicidic activity 2-like 2 (S. cerevisiae) (Skiv2l2), (10 ug)
ACCESSION   NM_001034093
VERSION     NM_001034093.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4164..4255 
                     /label="Myc-DDK"  
     misc_feature    4285..4870 
                     /label="PolyA signal"  
     misc_feature    4342..4361 
                     /label="Restriction Enzyme"  
     misc_feature    5146..5818 
                     /label="ColE1"  
     misc_feature    6248..7042 
                     /label="Kan/Neo"  
     misc_feature    7077..7379 
                     /label="SV40 ori"  
     misc_feature    7465..7942 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4146..4152 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4146 
                     /label="Skiv2l2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGACGCT TTCGGGGATG AGCTTTTCAG CGTTTTCGAG GATGATTCGA 
     1081 CCACTGCAGC GGGAACCAAG AAAGACAAAG AAAAGGAGAA AGATAAATGG AAGGGGCCAC 
     1141 CGGGGCCTGC AGACAAGGCC GGGAAACGAC TGGATACCAG ATTACAGTCA GAACCAGCCA 
     1201 GTAGTGGGAA AAACAAGAGA GATCTAGATG TTGAAGGCAC AGATGAACCT ATTTTTGGCA 
     1261 AGAAGCCCAG GATAGAAGAC TCAATTAATG AAGATTTAAG CTTGGCGGAC CTGATGCCCA 
     1321 GAGTCAAGGT ACAGTCGGTT GAAACAGTTG AAGGCTGTAC ACACGAGGTC GCTCTTCCTG 
     1381 CAGATGAGGA TTATATACCA CTTAAGCCTC GAGTTGGGAA GGCAGCAAAG GAATACCCGT 
     1441 TCATTCTTGA TGCTTTCCAA AGAGAAGCCA TTCAGTGTGT TGATAACAAT CAGTCTGTGT 
     1501 TAGTATCTGC TCATACATCA GCGGGGAAAA CCGTGTGCGC TGAGTATGCT ATTGCACTGG 
     1561 CTTTAAGGGA AAAACAGCGT GTAATATTTA CCAGCCCAAT AAAGGCTCTA AGTAATCAGA 
     1621 AATACCGTGA AATGTATGAA GAATTTCAAG ATGTTGGTCT GATGACTGGA GATGTTACTA 
     1681 TTAATCCCAC AGCATCTTGT CTGGTTATGA CTACAGAGAT TTTGAGAAGT ATGCTGTACA 
     1741 GAGGCTCTGA AGTTATGCGA GAAGTTGCGT GGGTCATATT TGATGAAATT CATTACATGA 
     1801 GAGATTCAGA GCGTGGTGTA GTATGGGAAG AAACTATTAT TTTGCTTCCA GACAATGTTC 
     1861 ACTATGTCTT TCTTTCGGCT ACTATTCCCA ATGCTCGACA GTTTGCTGAG TGGATCTGCC 
     1921 ACCTACATAA ACAGCCCTGT CATGTAATTT ATACTGATTA TCGACCTACT CCATTGCAAC 
     1981 ACTACATATT TCCAGCAGGG GGAGATGGCC TTCACCTTGT GGTTGATGAA AATGGTGACT 
     2041 TCAGAGAAGA TAATTTTAAC ACTGCAATGC AAGTACTTCG GGATGCAGGT GATTTGGCCA 
     2101 AAGGAGACCA GAAGGGACGG AAAGGAGGAA CAAAAGGTCC ATCAAATGTG TTCAAGATTG 
     2161 TGAAGATGAT TATGGAAAGA AATTTTCAAC CTGTGATTAT CTTCAGTTTT AGTAAGAAGG 
     2221 ATTGTGAAGC ATACGCCCTT CAAATGACTA AACTAGACTT CAACACAGAT GAAGAAAAGA 
     2281 AGATGGTTGA AGAAGTTTTC AATAATGCAA TTGACTGTTT GTCTGATGAA GATAAAAAGC 
     2341 TCCCTCAGGT TGAACATGTA CTTCCACTTT TGAAACGGGG GATTGGTATT CACCATGGTG 
     2401 GTTTGCTTCC TATTTTAAAG GAAACTATAG AGATTCTCTT TTCTGAAGGA TTGATAAAGG 
     2461 CCTTATTTGC CACAGAGACT TTTGCTATGG GAATTAACAT GCCAGCTAGA ACTGTTTTAT 
     2521 TTACAAATGC CCGTAAATAT GATGGGAAGG ATTTTAGATG GATTTCCTCA GGTGAGTACA 
     2581 TCCAGATGTC TGGTCGTGCA GGAAGGAGGG GGATGGATGA CAGAGGGATT GTGATTCTTA 
     2641 TGGTCGATGA AAAGATGAGC CCAACAATTG GAAAACAACT GCTTAAGGGC TCAGCTGATC 
     2701 CTCTGAATAG TGCTTTCCAT CTAACGTACA ACATGGTTTT GAACTTACTA AGAGTAGAAG 
     2761 AAATTAATCC CGAGTACATG CTTGAAAAAT CCTTCTACCA GTTTCAGCAT TATAGAGCAA 
     2821 TTCCAGGAGT TGTAGAAAAG GTAAAGAACT CGGAAGAACA GTATAATAAA ATAGTAATTC 
     2881 CCAATGAAGA AAATGTGGTC ATCTATTACA AGATTAGACA GCAACTTGCC AAGCTGGGAA 
     2941 AAGAGATTGA AGAATATATT CATAAGCCAA AATACTGCTT ACCCTTTCTA CAACCAGGCC 
     3001 GTTTAGTAAA GGTGAAGAAT GAAGGTGATG ATTTCGGCTG GGGAGTAGTG GTGAACTTCT 
     3061 CAAAAAAGTC AAACGTCAAG CCCAATTCTG GCGAACTGGA TCCCTTGTAT GTGGTGGAAG 
     3121 TGCTCCTGCG CTGTAGCAAG GAAAGCCTGA AAAACTCCGC CACAGAGGCA GCAAAGCCAG 
     3181 CAAAGCCGGA TGAGAAGGGG GAGATGCAGG TTGTTCCGGT TTTGGTGCAT CTCCTGTCTG 
     3241 CCATCAGCAC TGTTAGACTC TACATTCCAA AAGACCTCCG CCCCGTGGAC AACAGACAGA 
     3301 GTGTCCTAAA ATCCATACAG GAAGTTCAAA AGCGGTTTCC CGATGGTGTT CCGTTGTTAG 
     3361 ATCCTATCGA TGATATGGGC ATTCAAGATC AAGGACTGAA AAAAGTCATT CAGAAAGTAG 
     3421 AGGCTTTTGA GCATCGAATG TACTCGCACC CACTTCACAA TGATCCTAAT CTGGAAACTG 
     3481 TGTATACCCT TTGTGAAAAA AAGGCACAGA TTGCAATAGA TATTAAGTCT GCAAAGAGAG 
     3541 AACTGAAGAA AGCAAGAACT GTTCTGCAAA TGGATGAACT CAAATGTCGT AAGCGTGTTC 
     3601 TAAGAAGGCT GGGTTTTGCT ACATCTTCTG ATGTCATAGA GATGAAAGGA CGGGTGGCTT 
     3661 GTGAGATAAG CAGTGCTGAC GAGCTCCTTC TAACAGAGAT GATGTTTAAT GGGCTCTTCA 
     3721 ATGACCTTTC TTCAGAACAG GCGACCGCAC TGCTAAGCTG TTTTGTGTTT CAAGAGAATT 
     3781 CTAGTGAGAT GCCCAAATTA ACAGAGCAGT TAGCTGGACC ACTCCGACAA ATGCAGGAAT 
     3841 GTGCTAAAAG GATTGCAAAG GTTTCTGCAG AGGCAAAACT GGAGATTGAT GAAGAAACTT 
     3901 ACTTAAGCTC ATTCAAGCCT CACCTGATGG ATGTGGTGTA CACCTGGGCA ACTGGGGCCA 
     3961 CATTTGCCCA CATCTGCAAA ATGACAGATG TCTTTGAAGG CAGTATAATC CGTTGTATGA 
     4021 GGCGCCTGGA AGAACTGCTT CGACAGATGT GTCAAGCAGC GAAAGCCATT GGAAACACAG 
     4081 AGCTGGAGAA CAAGTTTGCT GAAGGAATCA CCAAAATCAA GAGGGATATT GTATTTGCTG 
     4141 CCAGCCTCTA CTTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4201 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4261 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4321 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4381 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4441 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4501 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4561 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4621 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4681 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4741 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4801 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4861 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4921 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4981 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5041 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5101 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5161 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5221 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5281 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5341 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5401 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5461 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5521 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5581 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5641 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5701 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5761 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5821 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5881 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5941 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6001 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6061 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6121 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6181 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6241 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6301 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6361 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6421 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6481 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6541 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6601 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6661 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6721 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6781 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6841 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6901 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6961 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7021 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7081 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7141 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7201 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7441 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7501 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7561 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7621 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7681 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7741 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7801 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7861 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7921 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7981 ATTTAACAAA AATTTAACGC GAATTTT
//