LOCUS       RG235089                9470 bp    DNA     circular  SYN 
DEFINITION  DDX11 (tGFP-tagged) - Homo sapiens DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 (DDX11), transcript variant 4
ACCESSION   NM_001257144
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3951..4655 
                     /label="GFP"  
     misc_feature    4702..5287 
                     /label="PolyA signal"  
     misc_feature    7379..8024 
                     /label="ColE1"  
     misc_feature    5741..6535 
                     /label="Neo"  
     misc_feature    5372..5674 
                     /label="SV40 ori"  
     misc_feature    8039..8899 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3930..3936 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3930 
                     /label="DDX11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTAATGAA ACACAGAAGG TTGGTGCCAT CCATTTTCCT TTTCCCTTCA 
     1081 CACCCTATTC CATCCAGGAA GACTTCATGG CAGAGCTGTA CCGGGTTTTG GAGGCTGGCA 
     1141 AGATTGGGAT ATTTGAGAGT CCAACTGGCA CTGGGAAGTC CTTAAGTCTT ATTTGTGGGG 
     1201 CCCTCTCTTG GCTCCGTGAC TTTGAACAGA AGAAGCGTGA AGAAGAGGCA CGACTCCTTG 
     1261 AAACTGGAAC TGGCCCCTTA CATGATGAGA AAGATGAATC CCTGTGTCTG TCTTCTTCCT 
     1321 GCGAAGGGGC TGCAGGCACC CCGAGGCCTG CTGGAGAACC GGCCTGGGTT ACTCAGTTTG 
     1381 TGCAGAAGAA AGAAGAGAGG GACCTGGTGG ACCGACTAAA GGCGGAGCAG GCCAGGAGGA 
     1441 AGCAGCGAGA AGAACGCCTG CAGCAGCTGC AGCACAGGGT GCAGCTCAAG TATGCAGCCA 
     1501 AGCGCCTGAG GCAGGAAGAA GAAGAAAGAG AGAATCTCCT CCGCCTCAGC AGGGAGATGC 
     1561 TAGAGACAGG CCCGGAGGCT GAGCGGCTGG AGCAGCTGGA GTCTGGGGAG GAGGAGCTGG 
     1621 TCCTCGCCGA ATACGAGAGT GATGAGGAGA AAAAGGTGGC GAGCAGAGTG GATGAGGATG 
     1681 AGGATGACCT GGAGGAAGAA CACATAACTA AGATTTATTA CTGTAGTCGG ACACACTCCC 
     1741 AGCTGGCCCA GTTTGTGCAT GAGGTGAAGA AGAGCCCCTT TGGCAAGGAT GTTCGGCTGG 
     1801 TCTCCCTTGG CTCCCGGCAG AACCTTTGTG TAAATGAAGA CGTGAAAAGC CTAGGTTCTG 
     1861 TGCAGCTTAT CAACGACCGC TGTGTGGACA TGCAGAGAAG CAGGCACGAG AAGAAGAAAG 
     1921 GAGCTGAGGA GGAGAAGCCA AAGAGGAGGA GGCAGGAGAA GCAGGCAGCC TGCCCCTTCT 
     1981 ACAACCACGA GCAGATGGGC CTTCTCCGGG ATGAGGCCCT GGCAGAGGTG AAGGACATGG 
     2041 AGCAGCTGCT GGCCCTTGGG AAGGAGGCCC GGGCCTGTCC CTATTACGGG AGCCGCCTTG 
     2101 CCATCCCTGC AGCCCAGCTG GTGGTGCTGC CCTATCAGAT GCTGCTGCAT GCGGCCACTC 
     2161 GGCAGGCCGC GGGCATCCGG CTGCAGGACC AGGTGGTGAT CATCGACGAG GCGCACAACC 
     2221 TGATCGACAC CATCACGGGC ATGCACAGCG TGGAGGTCAG CGGCTCCCAG CTCTGCCAGG 
     2281 CCCATTCCCA GCTGCTGCAG TACGTGGAGC GATACGGGAA GCGTTTGAAG GCCAAGAACC 
     2341 TGATGTACCT GAAGCAGATC CTGTATTTGC TGGAGAAATT CGTGGCTGTG CTAGGGGGGA 
     2401 ACATTAAGCA AAATCCCAAT ACACAGAGTC TGTCACAGAC AGGGACGGAG CTGAAGACCA 
     2461 TCAACGACTT TCTCTTCCAG AGCCAGATCG ACAACATCAA CCTGTTCAAG GTGCAGCGAT 
     2521 ACTGTGAGAA GAGCATGATC AGCAGAAAGC TCTTTGGATT CACTGAACGG TACGGAGCAG 
     2581 TGTTCTCATC CCGGGAGCAG CCCAAACTGG CTGGGTTTCA GCAATTCCTG CAGAGCCTGC 
     2641 AGCCCAGGAC GACTGAAGCT CTTGCAGCCC CTGCAGACGA GAGTCAGGCC AGCACCCTGC 
     2701 GACCAGCTTC TCCACTGATG CACATCCAAG GCTTCCTGGC AGCTCTCACT ACGGCCAACC 
     2761 AGGACGGCAG GGTCATCCTG AGCCGCCAAG GCAGCCTCAG TCAGAGCACC CTGAAGTTTT 
     2821 TGCTCCTGAA TCCAGCTGTG CACTTTGCCC AAGTGGTGAA GGAATGCCGG GCAGTGGTCA 
     2881 TTGCGGGGGG TACCATGCAG CCGGTGTCTG ACTTCCGGCA GCAGCTGCTG GCCTGTGCCG 
     2941 GGGTGGAAGC TGAGCGCGTG GTGGAGTTTT CCTGTGGTCA CGTGATCCCT CCAGACAACA 
     3001 TCCTGCCCCT CGTCATCTGC AGCGGGATCT CCAACCAGCC GCTGGAATTC ACGTTCCAGA 
     3061 AAAGAGAGCT GCCTCAGATG ATGGACGAGG TGGGTCGCAT TCTCTGTAAC CTGTGCGGTG 
     3121 TGGTTCCTGG AGGGGTGGTC TGTTTCTTCC CCTCCTACGA GTACCTGCGC CAGGTCCATG 
     3181 CCCACTGGGA GAAGGGTGGC CTGCTGGGCC GTCTGGCTGC CAGGAAGAAG ATATTCCAGG 
     3241 AACCTAAGAG CGCACACCAG GTGGAGCAGG TGCTGCTGGC ATATTCCAGG TGCATCCAGG 
     3301 CCTGTGGCCA GGAGAGAGGC CAGGTGACAG GGGCCCTGCT CCTCTCTGTG GTTGGAGGAA 
     3361 AGATGAGTGA AGGGATCAAC TTCTCTGACA ACCTAGGCCG GTGTGTGGTG ATGGTGGGCA 
     3421 TGCCCTTCCC CAACATCAGG TCTGCAGAGC TGCAGGAGAA GATGGCCTAC TTGGATCAAA 
     3481 CCCTCAGCCC CCGGCCAGGC ACCCCCAGGG AAGGCTCTGG TGGAGAACCT GTGCATGAAG 
     3541 GCCGTCAACC AGTCCATAGG CAGGGCCATC AGGCACCAGA AGGATTTTGC CAGCGTAGTG 
     3601 CTCCTGGACC AGCGATATGC CCGGCCCCCT GTCCTGGCCA AGCTGCCGGC CTGGATCCGA 
     3661 GCCCGTGTGG AGGTCAAAGC TACCTTTGGC CCCGCCATTG CTGCTGTGCA GAAGTTTCAC 
     3721 CGGGAGAAGT CGGCCTCTTC CTGATGGGCA ACCACACCAC TGCCTGGCGC CGTGCCCTTC 
     3781 CTTTGTCCTG CCCGCTGGAG ACAGTGTTTG TCGTGGGCGT GGTCTGCGGG GATCCTGTTA 
     3841 CAAAGGTGAA ACCCAGGAGG AGAGTGTGGA GTCCAGAGTG CTGCCAGGAC CCAGGCACAG 
     3901 GCGTTAGCTC CCGTAGGAGA AAATGGGGGA ATCCTGAAAC GCGTACGCGG CCGCTCGAGA 
     3961 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     4021 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     4081 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     4141 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     4201 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     4261 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     4321 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     4381 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     4441 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     4501 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     4561 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     4621 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     4681 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4741 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4801 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4861 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4921 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4981 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5041 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5101 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5161 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5221 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5281 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5341 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5401 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     5461 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     5521 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     5581 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     5641 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     5701 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     5761 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     5821 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     5881 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     5941 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     6001 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     6061 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     6121 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     6181 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     6241 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     6301 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     6361 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     6421 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     6481 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     6541 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     6601 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     6661 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     6721 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     6781 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     6841 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     6901 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     6961 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     7021 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     7081 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     7141 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     7201 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     7261 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     7321 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     7381 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     7441 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     7501 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     7561 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     7621 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     7681 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     7741 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     7801 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     7861 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     7921 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     7981 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     8041 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     8101 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     8161 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     8221 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     8281 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     8341 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     8401 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     8461 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     8521 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     8581 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     8641 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     8701 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     8761 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     8821 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     8881 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     8941 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     9001 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     9061 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     9121 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     9181 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     9241 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     9301 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     9361 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     9421 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//