LOCUS       RG231259                7982 bp    DNA     circular  SYN 
DEFINITION  PLD5 (tGFP-tagged) - Human phospholipase D family, member 5 (PLD5), transcript variant 2
ACCESSION   NM_001195811
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2463..3167 
                     /label="GFP"  
     misc_feature    3214..3799 
                     /label="PolyA signal"  
     misc_feature    5891..6536 
                     /label="ColE1"  
     misc_feature    4253..5047 
                     /label="Neo"  
     misc_feature    3884..4186 
                     /label="SV40 ori"  
     misc_feature    6551..7411 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2442..2448 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2442 
                     /label="PLD5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCAGCAG AAGTGCATCG TGATCTTTGC CCTGGTGTGC TGCTTTGCCA 
     1081 TTCTGGTTGC ACTGATCTTT TCAGCCGTGG ACATCATGGG AGAGGATGAG GATGGACTCT 
     1141 CAGAAAAAAA TTGCCAAAAT AAATGTCGAA TTGCCCTGGT GGAAAATATT CCTGAAGGCC 
     1201 TTAACTATTC AGAAAATGCA CCATTTCACT TATCACTTTT CCAAGGCTGG ATGAATTTAC 
     1261 TCAACATGGC CAAAAAGTCT GTTGACATAG TGTCTTCCCA TTGGGATCTC AACCACACTC 
     1321 ATCCATCAGC ATGTCAGGGT CAACGTCTTT TTGAAAAGTT GCTCCAGCTG ACTTCGCAAA 
     1381 ATATTGAAAT CAAGCTAGTG AGTGATGTAA CAGCTGATTC AAAGGTATTA GAAGCCTTGA 
     1441 AATTAAAGGG AGCCGAGGTG ACGTACATGA ACATGACCGC TTACAACAAG GGCCGGCTGC 
     1501 AGTCCTCCTT CTGGATCGTG GACAAACAGC ACGTGTATAT CGGCAGTGCC GGTTTGGACT 
     1561 GGCAATCCCT GGGACAGATG AAAGAACTCG GTGTCATCTT CTACAACTGC AGCTGCCTGG 
     1621 TCCTAGATTT ACAAAGGATA TTTGCTCTAT ATAGTTCATT AAAATTCAAA AGCAGAGTGC 
     1681 CTCAAACCTG GTCCAAAAGA CTCTATGGAG TCTATGACAA TGAAAAGAAA TTGCAACTTC 
     1741 AGTTGAATGA AACCAAATCT CAAGCATTTG TATCGAATTC TCCAAAACTC TTTTGCCCTA 
     1801 AAAACAGAAG TTTTGACATA GATGCCATCT ACAGTGTGAT AGATGATGCC AAGCAGTATG 
     1861 TGTACATCGC TGTCATGGAC TACCTGCCTA TCTCCAGCAC AAGCACCAAA AGGACTTACT 
     1921 GGCCAGACTT GGATGCAAAA ATAAGAGAAG CATTAGTTTT ACGAAGCGTT AGAGTTCGAC 
     1981 TCCTTTTAAG CTTCTGGAAG GAAACTGATC CCCTTACGTT TAACTTTATT TCATCTCTTA 
     2041 AAGCGATTTG CACTGAAATA GCCAACTGCA GTTTGAAAGT TAAATTTTTT GATCTGGAAA 
     2101 GAGAGAATGC TTGTGCTACA AAAGAACAAA AGAATCACAC CTTTCCTAGG TTAAATCGCA 
     2161 ACAAGTACAT GGTGACAGAT GGAGCAGCTT ATATTGGAAA TTTTGATTGG GTAGGGAATG 
     2221 ATTTCACTCA GAATGCTGGC ACGGGCCTTG TTATCAACCA GGCAGATGTG AGGAACAACA 
     2281 GAAGCATCAT TAAGCAACTT AAAGATGTGT TTGAAAGGGA CTGGTATTCA CCGTATGCCA 
     2341 AAACCTTACA GCCAACCAAA CAGCCGAACT GCTCAAGCCT GTTCAAACTC AAACCCCTCT 
     2401 CCAACAAAAC TGCCACAGAC GACACAGGCG GAAAGGATCC CCGGAACGTA ACGCGTACGC 
     2461 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     2521 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     2581 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     2641 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     2701 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     2761 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     2821 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     2881 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     2941 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     3001 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     3061 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     3121 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     3181 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3241 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3301 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3361 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3421 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3481 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3541 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3601 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3661 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3721 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3781 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3841 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     3901 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     3961 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     4021 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     4081 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     4141 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     4201 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     4261 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     4321 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     4381 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     4441 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     4501 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     4561 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     4621 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     4681 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     4741 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     4801 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     4861 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     4921 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     4981 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     5041 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     5101 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     5161 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     5221 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     5281 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     5341 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     5401 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     5461 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     5521 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     5581 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     5641 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     5701 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     5761 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     5821 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     5881 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     5941 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     6001 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     6061 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     6121 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     6181 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     6241 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     6301 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     6361 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     6421 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     6481 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     6541 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     6601 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     6661 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     6721 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     6781 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     6841 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     6901 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     6961 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     7021 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     7081 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     7141 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     7201 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     7261 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     7321 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     7381 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     7441 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     7501 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     7561 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     7621 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     7681 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     7741 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     7801 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     7861 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     7921 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     7981 TT
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