LOCUS       RG230559                9203 bp    DNA     circular  SYN 
DEFINITION  CLCN2 (tGFP-tagged) - Human chloride channel 2 (CLCN2), transcript variant 2
ACCESSION   NM_001171087
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3684..4388 
                     /label="GFP"  
     misc_feature    4435..5020 
                     /label="PolyA signal"  
     misc_feature    7112..7757 
                     /label="ColE1"  
     misc_feature    5474..6268 
                     /label="Neo"  
     misc_feature    5105..5407 
                     /label="SV40 ori"  
     misc_feature    7772..8632 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3663..3669 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3663 
                     /label="CLCN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCGCG GCGGCGGAGG AAGGGATGGA GCCACGGGCG CTGCAGTACG 
     1081 AGCAGACCCT GATGTATGGC CGGTACACTC AGGACCTTGG GGCCTTTGCC AAAGAGGAAG 
     1141 CTGCTCGGAT TCGCCTGGGA GGGCCTGAAC CCTGGAAAGG TCCCCCTTCC TCTCGGGCTG 
     1201 CCCCAGAGCT CTTGGAATAT GGACGGAGCC GTTGCGCCCG ATGCCGCGTC TGTTCTGTCC 
     1261 GCTGCCACAA GTTCCTAGTA TCCAGGGTTG GTGAAGATTG GATCTTCCTG GTCCTGCTGG 
     1321 GGCTTCTCAT GGCATTGGTC AGCTGGGTCA TGGACTATGC CATTGCTGCC TGTCTGCAAG 
     1381 CCCAGCAGTG GATGTCCCGG GGCTTGAACA CCAGCATCTT GCTCCAGTAC CTGGCCTGGG 
     1441 TCACCTACCC TGTTGTCCTC ATCACTTTCT CAGCCGGATT CACACAGATC CTGGCCCCTC 
     1501 AGGCTGTCGG CTCTGGCATC CCTGAGATGA AGACCATCTT GCGGGGAGTG GTGCTGAAAG 
     1561 AATACCTCAC ACTCAAGACC TTTATAGCTA AGGTCATTGG GCTGACCTGC GCCCTAGGCA 
     1621 GCGGGATGCC GCTTGGCAAA GAGGGCCCTT TTGTGCATAT CGCAAGCATG TGTGCTGCCC 
     1681 TTCTCAGCAA GTTCCTCTCC CTCTTTGGGG GTATCTATGA GAATGAATCC CGGAACACAG 
     1741 AGATGCTGGC TGCCGCCTGT GCCGTGGGGG TGGGCTGCTG CTTCGCGGCA CCTATTGGAG 
     1801 GCGTCCTCTT CAGCATCGAG GTCACCTCCA CCTTCTTTGC AGTGCGGAAC TACTGGCGGG 
     1861 GCTTCTTCGC TGCCACCTTC AGTGCCTTCA TCTTCCGGGT CTTGGCAGTC TGGAACCGGG 
     1921 ATGAAGAGAC TATTACAGCC CTCTTCAAAA CCCGATTCCG GCTCGACTTC CCCTTTGACC 
     1981 TGCAGGAGCT GCCAGCCTTT GCTGTCATTG GTATTGCTAG TGGCTTCGGT GGAGCCCTCT 
     2041 TTGTCTACCT GAACCGGAAG ATTGTCCAGG TGATGCGGAA GCAGAAAACC ATCAATCGCT 
     2101 TCCTCATGAG GAAACGCCTG CTCTTCCCGG CTCTGGTGAC CCTGCTCATC TCCACGCTGA 
     2161 CCTTCCCCCC TGGCTTTGGA CAGTTCATGG CTGGACAGCT CTCACAGAAA GAGACGCTGG 
     2221 TCACCCTGTT TGACAATCGG ACGTGGGTCC GCCAGGGCCT GGTGGAGGAG CTAGAACCAC 
     2281 CCAGCACCTC ACAGGCCTGG AACCCACCAC GTGCCAACGT CTTCCTCACC CTGGTCATCT 
     2341 TCATTCTCAT GAAGTTCTGG ATGTCTGCAC TGGCCACCAC CATCCCAGTT CCCTGTGGGG 
     2401 CCTTCATGCC TGTCTTTGTC ATTGATGGAA TTCATACGGA CAGCAGCACC TACCGGATTG 
     2461 TGCCTGGGGG CTACGCTGTG GTCGGGGCAG CTGCGCTGGC AGGAGCGGTG ACACACACAG 
     2521 TGTCCACGGC TGTGATCGTG TTCGAGCTCA CAGGCCAGAT TGCCCACATC CTGCCTGTCA 
     2581 TGATCGCCGT CATCCTGGCC AACGCTGTCG CCCAGAGTCT GCAGCCCTCC CTCTATGACA 
     2641 GCATCATCCG AATCAAGAAA CTGCCCTACC TGCCTGAGCT CGGCTGGGGC CGCCACCAGC 
     2701 AGTACCGGGT GCGTGTGGAG GACATCATGG TGCGGGATGT TCCCCATGTG GCCCTCAGCT 
     2761 GCACCTTCCG GGACCTGCGT TTGGCACTGC ACAGGACCAA GGGCCGAATG CTGGCCCTAG 
     2821 TGGAGTCCCC TGAGTCCATG ATTCTGCTGG GCTCCATCGA GCGTTCACAG GTGGTGGCAT 
     2881 TGTTGGGGGC CCAGCTGAGC CCAGCCCGCC GGCGGCAGCA CATGCAGGAG CGCAGAGCCA 
     2941 CCCAGACCTC TCCACTATCT GATCAGGAGG GTCCCCCTAC CCCTGAGGCT TCTGTCTGCT 
     3001 TCCAGGTGAA CACAGAAGAC TCAGCCTTCC CAGCAGCCCG GGGGGAGACC CACAAGCCCC 
     3061 TAAAGCCTGC ACTCAAGAGG GGGCCCAGTG TCACCAGGAA CCTCGGAGAG AGTCCCACAG 
     3121 GGAGCGCAGA GTCGGCAGGC ATCGCCCTCC GGAGCCTCTT CTGTGGCAGT CCACCCCCTG 
     3181 AGGCTGCTTC GGAGAAGTTG GAATCCTGTG AGAAGCGCAA GCTGAAGCGT GTCCGAATCT 
     3241 CCCTGGCAAG TGACGCGGAC CTGGAAGGCG AGATGAGCCC TGAAGAGATT CTGGAGTGGG 
     3301 AGGAGCAGCA ACTAGATGAA CCTGTCAACT TCAGTGACTG CAAAATTGAT CCTGCTCCCT 
     3361 TCCAGCTGGT GGAGCGGACC TCTTTGCACA AGACTCACAC TATCTTCTCA CTGCTGGGAG 
     3421 TGGACCATGC TTATGTCACC AGTATTGGCA GACTCATTGG AATCGTTACT CTAAAGGAGC 
     3481 TCCGGAAGGC CATCGAGGGC TCTGTCACAG CACAGGGTGT GAAAGTCCGG CCGCCCCTCG 
     3541 CCAGCTTCCG AGACAGTGCC ACCAGCAGCA GTGACACGGA GACCACTGAG GTGCATGCAC 
     3601 TCTGGGGGCC CCACTCCCGT CATGGCCTCC CCCGGGAGGG CAGCCCTTCC GACAGCGACG 
     3661 ACAAATGCCA AACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3721 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3781 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3841 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     3901 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     3961 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4021 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4081 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4141 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4201 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4261 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4321 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4381 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4441 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4501 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4561 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4621 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4681 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4741 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4801 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4861 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4921 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4981 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5041 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5101 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5161 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5221 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5281 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5341 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5401 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5461 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5521 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5581 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5641 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5701 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5761 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5821 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     5881 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     5941 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6001 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6061 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6121 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6181 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6241 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6301 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6361 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6421 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6481 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6541 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6601 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6661 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6721 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6781 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6841 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     6901 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     6961 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7021 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7081 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7141 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7201 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7261 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7321 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7381 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7441 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7501 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7561 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7621 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7681 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7741 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7801 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7861 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     7921 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     7981 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8041 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8101 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8161 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8221 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8281 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8341 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8401 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8461 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8521 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8581 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8641 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8701 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8761 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8821 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     8881 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     8941 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9001 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9061 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9121 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9181 AACAAAAATT TAACGCGAAT TTT
//