LOCUS       RG230123                7940 bp    DNA     circular  SYN 
DEFINITION  IDS (tGFP-tagged) - Human iduronate 2-sulfatase (IDS), transcript variant 3
ACCESSION   NM_001166550
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2421..3125 
                     /label="GFP"  
     misc_feature    3172..3757 
                     /label="PolyA signal"  
     misc_feature    5849..6494 
                     /label="ColE1"  
     misc_feature    4211..5005 
                     /label="Neo"  
     misc_feature    3842..4144 
                     /label="SV40 ori"  
     misc_feature    6509..7369 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2400..2406 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2400 
                     /label="IDS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTTTGCGC AGGAGACCTG ACACCACCCG CCTGTACGAC TTCAACTCCT 
     1081 ACTGGAGGGT GCACGCTGGA AACTTCTCCA CCATCCCCCA GTACTTCAAG GAGAATGGCT 
     1141 ATGTGACCAT GTCGGTGGGA AAAGTCTTTC ACCCTGGGAT ATCTTCTAAC CATACCGATG 
     1201 ATTCTCCGTA TAGCTGGTCT TTTCCACCTT ATCATCCTTC CTCTGAGAAG TATGAAAACA 
     1261 CTAAGACATG TCGAGGGCCA GATGGAGAAC TCCATGCCAA CCTGCTTTGC CCTGTGGATG 
     1321 TGCTGGATGT TCCCGAGGGC ACCTTGCCTG ACAAACAGAG CACTGAGCAA GCCATACAGT 
     1381 TGTTGGAAAA GATGAAAACG TCAGCCAGTC CTTTCTTCCT GGCCGTTGGG TATCATAAGC 
     1441 CACACATCCC CTTCAGATAC CCCAAGGAAT TTCAGAAGTT GTATCCCTTG GAGAACATCA 
     1501 CCCTGGCCCC CGATCCCGAG GTCCCTGATG GCCTACCCCC TGTGGCCTAC AACCCCTGGA 
     1561 TGGACATCAG GCAACGGGAA GACGTCCAAG CCTTAAACAT CAGTGTGCCG TATGGTCCAA 
     1621 TTCCTGTGGA CTTTCAGCGG AAAATCCGCC AGAGCTACTT TGCCTCTGTG TCATATTTGG 
     1681 ATACACAGGT CGGCCGCCTC TTGAGTGCTT TGGACGATCT TCAGCTGGCC AACAGCACCA 
     1741 TCATTGCATT TACCTCGGAT CATGGGTGGG CTCTAGGTGA ACATGGAGAA TGGGCCAAAT 
     1801 ACAGCAATTT TGATGTTGCT ACCCATGTTC CCCTGATATT CTATGTTCCT GGAAGGACGG 
     1861 CTTCACTTCC GGAGGCAGGC GAGAAGCTTT TCCCTTACCT CGACCCTTTT GATTCCGCCT 
     1921 CACAGTTGAT GGAGCCAGGC AGGCAATCCA TGGACCTTGT GGAACTTGTG TCTCTTTTTC 
     1981 CCACGCTGGC TGGACTTGCA GGACTGCAGG TTCCACCTCG CTGCCCCGTT CCTTCATTTC 
     2041 ACGTTGAGCT GTGCAGAGAA GGCAAGAACC TTCTGAAGCA TTTTCGATTC CGTGACTTGG 
     2101 AAGAGGATCC GTACCTCCCT GGTAATCCCC GTGAACTGAT TGCCTATAGC CAGTATCCCC 
     2161 GGCCTTCAGA CATCCCTCAG TGGAATTCTG ACAAGCCGAG TTTAAAAGAT ATAAAGATCA 
     2221 TGGGCTATTC CATACGCACC ATAGACTATA GGTATACTGT GTGGGTTGGC TTCAATCCTG 
     2281 ATGAATTTCT AGCTAACTTT TCTGACATCC ATGCAGGGGA ACTGTATTTT GTGGATTCTG 
     2341 ACCCATTGCA GGATCACAAT ATGTATAATG ATTCCCAAGG TGGAGATCTT TTCCAGTTGT 
     2401 TGATGCCTAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     2461 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     2521 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     2581 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     2641 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     2701 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     2761 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     2821 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     2881 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     2941 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     3001 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     3061 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     3121 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3181 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3241 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3301 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3361 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3421 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3481 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3541 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3601 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3661 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3721 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3781 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     3841 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     3901 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     3961 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     4021 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     4081 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     4141 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     4201 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     4261 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     4321 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     4381 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     4441 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     4501 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     4561 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     4621 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     4681 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     4741 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     4801 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     4861 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     4921 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     4981 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     5041 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     5101 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     5161 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     5221 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     5281 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     5341 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     5401 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     5461 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     5521 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     5581 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     5641 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     5701 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     5761 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     5821 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     5881 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     5941 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     6001 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     6061 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     6121 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     6181 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     6241 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     6301 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     6361 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     6421 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     6481 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     6541 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     6601 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     6661 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     6721 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     6781 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     6841 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     6901 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     6961 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     7021 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     7081 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     7141 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     7201 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     7261 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     7321 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     7381 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     7441 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     7501 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     7561 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     7621 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     7681 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     7741 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     7801 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     7861 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     7921 AAAAATTTAA CGCGAATTTT 
//