LOCUS       RG229400                9518 bp    DNA     circular  SYN 
DEFINITION  ANO1 (tGFP-tagged) - Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1
ACCESSION   NM_018043
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3999..4703 
                     /label="GFP"  
     misc_feature    4750..5335 
                     /label="PolyA signal"  
     misc_feature    7427..8072 
                     /label="ColE1"  
     misc_feature    5789..6583 
                     /label="Neo"  
     misc_feature    5420..5722 
                     /label="SV40 ori"  
     misc_feature    8087..8947 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3978..3984 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3978 
                     /label="ANO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGTCAAC GAGAAGTACT CGACGCTCCC GGCCGAGGAC CGCAGCGTCC 
     1081 ACATCATCAA CATCTGCGCC ATCGAGGACA TCGGCTACCT GCCGTCCGAG GGCACGCTGC 
     1141 TGAACTCCTT ATCTGTGGAC CCTGATGCCG AGTGCAAGTA TGGCCTGTAC TTCAGGGACG 
     1201 GCCGGCGCAA GGTGGACTAC ATCCTGGTGT ACCATCACAA GAGGCCCTCG GGCAACCGGA 
     1261 CCCTGGTCAG GAGGGTGCAG CACAGCGACA CCCCCTCTGG GGCTCGCAGC GTCAAGCAGG 
     1321 ACCACCCCCT GCCGGGCAAG GGGGCGTCGC TGGATGCAGG CTCGGGGGAG CCCCCGATGG 
     1381 ACTACCACGA GGATGACAAG CGCTTCCGCA GGGAGGAGTA CGAGGGCAAC CTCCTGGAGG 
     1441 CGGGCCTGGA GCTGGAGCGG GACGAGGACA CTAAAATCCA CGGAGTCGGG TTTGTGAAAA 
     1501 TCCATGCCCC CTGGAACGTG CTGTGCAGAG AGGCCGAGTT TCTGAAACTG AAGATGCCGA 
     1561 CGAAGAAGAT GTACCACATT AATGAGACCC GTGGCCTCCT GAAAAAAATC AACTCTGTGC 
     1621 TCCAGAAAAT CACAGATCCC ATCCAGCCCA AAGTGGCTGA GCACAGGCCC CAGACCATGA 
     1681 AGAGACTCTC CTATCCCTTC TCCCGGGAGA AGCAGCATCT ATTTGACTTG TCTGATAAGG 
     1741 ATTCCTTTTT CGACAGCAAA ACCCGGAGCA CGATTGTCTA TGAGATCTTG AAGAGAACGA 
     1801 CGTGTACAAA GGCCAAGTAC AGCATGGGCA TCACGAGCCT GCTGGCCAAT GGTGTGTACG 
     1861 CGGCTGCATA CCCACTGCAC GATGGAGACT ACAACGGTGA AAACGTCGAG TTCAACGACA 
     1921 GAAAACTCCT GTACGAAGAG TGGGCACGCT ATGGAGTTTT CTATAAGTAC CAGCCCATCG 
     1981 ACCTGGTCAG GAAGTATTTT GGGGAGAAGA TCGGCCTGTA CTTCGCCTGG CTGGGCGTGT 
     2041 ACACCCAGAT GCTCATCCCT GCCTCCATCG TGGGAATCAT TGTCTTCCTG TACGGATGCG 
     2101 CCACCATGGA TGAAAACATC CCCAGCATGG AGATGTGTGA CCAGAGACAC AATATCACCA 
     2161 TGTGCCCGCT TTGCGACAAG ACCTGCAGCT ACTGGAAGAT GAGCTCAGCC TGCGCCACGG 
     2221 CCCGCGCCAG CCACCTCTTC GACAACCCCG CCACGGTCTT CTTCTCTGTC TTCATGGCCC 
     2281 TCTGGGCTGC CACCTTCATG GAGCACTGGA AGCGGAAACA GATGCGACTC AACTACCGCT 
     2341 GGGACCTCAC GGGCTTTGAA GAGGAAGAGG AGGCTGTCAA GGATCATCCT AGAGCTGAAT 
     2401 ACGAAGCCAG AGTCTTGGAG AAGTCTCTGA AGAAAGAGTC CAGAAACAAA GAGAAGCGCC 
     2461 GGCATATTCC AGAGGAGTCA ACAAACAAAT GGAAGCAGAG GGTTAAGACA GCCATGGCGG 
     2521 GGGTGAAATT GACTGACAAA GTGAAGCTGA CATGGAGAGA TCGGTTCCCA GCCTACCTCA 
     2581 CTAACTTGGT CTCCATCATC TTCATGATTG CAGTGACGTT TGCCATCGTC CTCGGCGTCA 
     2641 TCATCTACAG AATCTCCATG GCCGCCGCCT TGGCCATGAA CTCCTCCCCC TCCGTGCGGT 
     2701 CCAACATCCG GGTCACAGTC ACAGCCACCG CAGTCATCAT CAACCTAGTG GTCATCATCC 
     2761 TCCTGGACGA GGTGTATGGC TGCATAGCCC GATGGCTCAC CAAGATCGAG GTCCCAAAGA 
     2821 CGGAGAAAAG CTTTGAGGAG AGGCTGATCT TCAAGGCTTT CCTGCTGAAG TTTGTGAATT 
     2881 CCTACACCCC CATCTTTTAC GTGGCGTTCT TCAAAGGCCG GTTTGTTGGA CGCCCGGGCG 
     2941 ACTACGTGTA CATTTTCCGT TCCTTCCGAA TGGAAGAGTG TGCGCCAGGG GGCTGCCTGA 
     3001 TGGAGCTATG CATCCAGCTC AGCATCATCA TGCTGGGGAA ACAGCTGATC CAGAACAACC 
     3061 TGTTCGAGAT CGGCATCCCG AAGATGAAGA AGCTCATCCG CTACCTGAAG CTGAAGCAGC 
     3121 AGAGCCCCCC TGACCACGAG GAGTGTGTGA AGAGGAAACA GCGGTACGAG GTGGATTACA 
     3181 ACCTGGAGCC CTTCGCGGGC CTCACCCCAG AGTACATGGA AATGATCATC CAGTTTGGCT 
     3241 TCGTCACCCT GTTTGTCGCC TCCTTCCCCC TGGCCCCACT GTTTGCGCTG CTGAACAACA 
     3301 TCATCGAGAT CCGCCTGGAC GCCAAAAAGT TTGTCACTGA GCTCCGAAGG CCGGTAGCTG 
     3361 TCAGAGCCAA AGACATCGGA ATCTGGTACA ATATCCTCAG AGGCATTGGG AAGCTTGCTG 
     3421 TCATCATCAA TGCCTTCGTG ATCTCCTTCA CGTCTGACTT CATCCCGCGC CTGGTGTACC 
     3481 TCTACATGTA CAGTAAGAAC GGGACCATGC ACGGCTTCGT CAACCACACC CTCTCCTCCT 
     3541 TCAACGTCAG TGACTTCCAG AACGGCACGG CCCCCAATGA CCCCCTGGAC CTGGGCTACG 
     3601 AGGTGCAGAT CTGCAGGTAT AAAGACTACC GAGAGCCGCC GTGGTCGGAA AACAAGTACG 
     3661 ACATCTCCAA GGACTTCTGG GCCGTCCTGG CAGCCCGGCT GGCGTTTGTC ATCGTCTTCC 
     3721 AGAACCTGGT CATGTTCATG AGCGACTTTG TGGACTGGGT CATCCCGGAC ATCCCCAAGG 
     3781 ACATCAGCCA GCAGATCCAC AAGGAGAAGG TGCTCATGGT GGAGCTGTTC ATGCGGGAGG 
     3841 AGCAAGACAA GCAGCAGCTG CTGGAAACCT GGATGGAGAA GGAGCGGCAG AAGGACGAGC 
     3901 CGCCGTGCAA CCACCACAAC ACCAAAGCCT GCCCAGACAG CCTCGGCAGC CCAGCCCCCA 
     3961 GCCATGCCTA CCACGGGGGC GTCCTGACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     4021 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     4081 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     4141 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     4201 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     4261 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4321 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4381 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4441 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4501 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4561 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4621 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4681 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4741 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4801 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4861 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4921 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4981 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5041 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5101 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5161 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5221 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5281 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5341 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5401 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5461 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5521 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5581 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5641 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5701 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5761 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5821 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5881 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5941 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     6001 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     6061 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     6121 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     6181 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     6241 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6301 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6361 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6421 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6481 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6541 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6601 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6661 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6721 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6781 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6841 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6901 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6961 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     7021 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     7081 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     7141 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     7201 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     7261 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7321 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7381 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7441 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7501 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7561 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7621 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7681 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7741 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7801 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7861 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7921 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7981 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     8041 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     8101 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     8161 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     8221 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     8281 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8341 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8401 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8461 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8521 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8581 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8641 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8701 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8761 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8821 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8881 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8941 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     9001 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     9061 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     9121 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     9181 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     9241 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9301 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9361 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9421 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9481 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//