LOCUS       RG229397                9413 bp    DNA     circular  SYN 
DEFINITION  PCDH20 (tGFP-tagged) - Human protocadherin 20 (PCDH20)
ACCESSION   NM_022843
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3894..4598 
                     /label="GFP"  
     misc_feature    4645..5230 
                     /label="PolyA signal"  
     misc_feature    7322..7967 
                     /label="ColE1"  
     misc_feature    5684..6478 
                     /label="Neo"  
     misc_feature    5315..5617 
                     /label="SV40 ori"  
     misc_feature    7982..8842 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3873..3879 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3873 
                     /label="PCDH20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCGGCCGA GGGAATGCGC GCAGCTCACA GGCCCTGGGA GTGAGCTGGT 
     1081 GCCCGGCGAC CTGGCACCCG CGCCTGGATA TGGGGCGTCT ACATCGTCCC AGGAGCAGCA 
     1141 CCAGCTACAG GAACCTGCCG CATCTGTTTC TGTTTTTCCT CTTCGTGGGA CCCTTCAGCT 
     1201 GCCTCGGGAG TTACAGCCGG GCCACCGAGC TTCTGTACAG CCTAAACGAG GGACTACCCG 
     1261 CGGGGGTGCT CATCGGCAGC CTGGCCGAGG ACCTGCGGCT GCTGCCCAGG TCTGCAGGGA 
     1321 GGCCGGACCC GCAGTCGCAG CTGCCAGAGC GCACCGGTGC TGAGTGGAAC CCCCCTCTCT 
     1381 CCTTCAGCCT GGCCTCCCGG GGACTGAGTG GCCAGTACGT GACCCTAGAC AACCGCTCTG 
     1441 GGGAGCTGCA CACTTCAGCT CAGGAGATCG ACAGGGAGGC CCTGTGTGTT GAAGGGGGTG 
     1501 GAGGGACTGC GTGGAGCGGC AGCGTTTCCA TCTCCTCCTC TCCTTCTGAC TCTTGTCTTT 
     1561 TGCTGCTGGA TGTGCTTGTC CTGCCTCAGG AATACTTCAG GTTTGTGAAG GTGAAGATCG 
     1621 CCATCAGAGA CATCAATGAC AACGCCCCGC AGTTCCCTGT TTCCCAGATC TCGGTGTGGG 
     1681 TCCCGGAAAA TGCACCTGTA AACACCCGAC TGGCCATAGA GCATCCTGCT GTGGACCCAG 
     1741 ATGTAGGCAT TAATGGGGTA CAGACCTATC GCTTACTGGA CTACCATGGT ATGTTCACCC 
     1801 TGGACGTGGA GGAGAATGAG AATGGGGAGC GCACCCCCTA CCTAATTGTC ATGGGTGCTT 
     1861 TGGACAGGGA AACCCAGGAC CAGTATGTGA GCATCATCAT AGCTGAGGAT GGTGGGTCTC 
     1921 CACCACTTTT GGGCAGTGCC ACTCTCACCA TTGGCATCAG TGACATTAAT GACAATTGCC 
     1981 CTCTCTTCAC AGACTCACAA ATCAATGTCA CTGTGTATGG GAATGCTACA GTGGGCACCC 
     2041 CAATTGCAGC TGTCCAGGCT GTGGATAAAG ACTTGGGGAC CAATGCTCAA ATTACTTATT 
     2101 CTTACAGTCA GAAAGTTCCA CAAGCATCTA AGGATTTATT TCACCTGGAT GAAAACACTG 
     2161 GAGTCATTAA ACTTTTCAGT AAGATTGGAG GAAGTGTTCT GGAGTCCCAC AAGCTCACCA 
     2221 TCCTTGCTAA TGGACCAGGC TGCATCCCTG CTGTAATCAC TGCTCTTGTG TCCATTATTA 
     2281 AAGTTATTTT CAGACCCCCT GAAATTGTCC CTCGTTACAT AGCAAACGAG ATAGATGGTG 
     2341 TGGTTTATCT GAAAGAACTG GAACCCGTTA ACACTCCCAT TGCGTTTTTC ACCATAAGAG 
     2401 ATCCAGAAGG TAAATACAAG GTTAACTGCT ACCTGGATGG TGAAGGGCCG TTTAGGTTAT 
     2461 CACCTTACAA ACCATACAAT AATGAATATT TACTAGAGAC CACAAAACCT ATGGACTATG 
     2521 AGCTACAGCA GTTCTATGAA GTAGCTGTGG TGGCTTGGAA CTCTGAGGGA TTTCATGTCA 
     2581 AAAGGGTCAT TAAAGTGCAA CTTTTAGATG ACAATGATAA TGCTCCAATT TTCCTTCAAC 
     2641 CCTTAATAGA ACTAACCATC GAAGAGAACA ACTCACCCAA TGCCTTTTTG ACTAAGCTGT 
     2701 ATGCTACAGA TGCCGACAGC GAGGAGAGAG GCCAAGTTTC ATATTTTCTG GGACCTGATG 
     2761 CTCCATCATA TTTTTCCTTA GACAGTGTCA CAGGAATTCT GACAGTTTCT ACTCAGCTGG 
     2821 ACCGAGAAGA GAAAGAAAAG TACAGATACA CTGTCAGAGC TGTTGACTGT GGGAAGCCAC 
     2881 CCAGAGAATC AGTAGCCACT GTGGCCCTCA CAGTGTTGGA TAAAAATGAC AACAGTCCTC 
     2941 GGTTTATCAA CAAGGACTTC AGCTTTTTTG TGCCTGAAAA CTTTCCAGGC TATGGTGAGA 
     3001 TTGGAGTAAT TAGTGTAACA GATGCTGACG CTGGACGAAA TGGATGGGTC GCCCTCTCTG 
     3061 TGGTGAACCA GAGTGATATT TTTGTCATAG ATACAGGAAA GGGTATGCTG AGGGCTAAAG 
     3121 TCTCTTTGGA CAGAGAGCAG CAAAGCTCCT ATACTTTGTG GGTTGAAGCT GTTGATGGGG 
     3181 GTGAGCCTGC CCTCTCCTCT ACAGCAAAAA TCACAATTCT CCTTCTAGAT ATCAATGACA 
     3241 ACCCTCCTCT TGTTTTGTTT CCTCAGTCTA ATATGTCTTA TCTGTTAGTA CTGCCTTCTA 
     3301 CTCTGCCAGG CTCCCCGGTT ACAGAAGTCT ATGCTGTCGA CAAAGACACA GGCATGAATG 
     3361 CTGTCATAGC TTACAGCATC ATAGGGAGAA GAGGTCCTAG GCCTGAGTCC TTCAGGATTG 
     3421 ACCCTAAAAC TGGCAACATT ACTTTGGAAG AGGCATTGCT GCAGACAGAT TATGGGCTCC 
     3481 ATCGCTTACT GGTGAAAGTG AGTGATCATG GTTATCCCGA GCCTCTCCAC TCCACAGTCA 
     3541 TGGTGAACCT ATTTGTCAAT GACACTGTCA GTAATGAGAG TTACATTGAG AGTCTTTTAA 
     3601 GAAAAGAACC AGAGATTAAT ATAGAGGAGA AAGAACCACA AATCTCAATA GAACCGACTC 
     3661 ATAGGAAGGT AGAATCTGTG TCTTGTATGC CCACCTTAGT AGCTCTGTCT GTAATAAGCT 
     3721 TGGGTTCCAT CACACTGGTC ACAGGGATGG GCATATACAT CTGTTTAAGG AAAGGGGAAA 
     3781 AGCATCCCAG GGAAGATGAA AATTTGGAAG TACAGATTCC ACTGAAAGGA AAAATTGACT 
     3841 TGCATATGCG AGAGAGAAAG CCAATGGATA TTTCTAATAT TACGCGTACG CGGCCGCTCG 
     3901 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3961 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     4021 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     4081 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     4141 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     4201 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     4261 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     4321 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     4381 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     4441 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4501 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4561 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5341 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     5401 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     5461 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5521 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5581 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5641 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5701 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5761 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5821 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5881 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5941 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     6001 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     6061 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     6121 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     6181 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     6241 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     6301 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     6361 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     6421 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6481 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6541 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6601 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6661 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6721 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6781 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6841 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6901 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6961 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     7021 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     7081 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     7141 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     7201 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     7261 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     7321 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     7381 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     7441 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7501 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7561 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7621 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7681 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7741 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7801 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7861 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7921 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7981 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     8041 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     8101 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     8161 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     8221 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     8281 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     8341 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     8401 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     8461 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8521 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8581 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8641 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8701 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8761 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8821 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8881 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8941 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     9001 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     9061 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     9121 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     9181 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     9241 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     9301 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     9361 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//