LOCUS       RG228619                9368 bp    DNA     circular  SYN 
DEFINITION  NPAS3 (tGFP-tagged) - Human neuronal PAS domain protein 3 (NPAS3), transcript variant 1
ACCESSION   NM_001164749
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3847..4551 
                     /label="GFP"  
     misc_feature    4598..5183 
                     /label="PolyA signal"  
     misc_feature    7275..7920 
                     /label="ColE1"  
     misc_feature    5637..6431 
                     /label="Neo"  
     misc_feature    5268..5570 
                     /label="SV40 ori"  
     misc_feature    7935..8795 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3819..3826 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3819 
                     /label="NPAS3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCCACC AAGCCCAGCT TTCAGCAGGA TCCTTCCAGG CGAGAACGAA 
     1081 TAACTGCCCA GCATCCTCTG CCCAACCAAT CAGAATGTAG GAAAATCTAC AGATATGACG 
     1141 GAATCTACTG TGAATCTACC TACCAGAATT TACAAGCATT GAGAAAGGAG AAATCCCGAG 
     1201 ATGCTGCTCG CTCCCGCCGG GGAAAAGAAA ACTTTGAGTT CTATGAATTG GCCAAGTTGT 
     1261 TGCCTCTTCC TGCAGCCATT ACCAGCCAGC TCGACAAGGC ATCCATCATT CGACTTACAA 
     1321 TTAGCTATCT GAAAATGAGG GACTTTGCTA ACCAGGGGGA CCCTCCGTGG AACTTGCGAA 
     1381 TGGAAGGCCC TCCACCTAAC ACATCAGTAA AAGTTATAGG TGCACAGCGA AGGAGAAGCC 
     1441 CCAGTGCACT AGCCATTGAA GTATTTGAAG CACATTTGGG AAGCCACATT TTGCAGTCCC 
     1501 TGGATGGCTT TGTATTTGCA CTAAATCAGG AAGGAAAATT TTTGTACATT TCCGAAACAG 
     1561 TCTCCATCTA CCTAGGCCTC TCACAAGTGG AGCTGACAGG CAGCAGTGTC TTTGACTATG 
     1621 TCCACCCCGG AGATCACGTG GAGATGGCTG AGCAGCTGGG CATGAAGCTC CCCCCTGGGC 
     1681 GGGGTCTCCT GTCACAGGGC ACTGCTGAGG ACGGAGCCAG CTCAGCATCT TCCTCCTCTC 
     1741 AGTCGGAGAC CCCCGAGCCA GTGGAGTCAA CCAGCCCCAG TCTGCTAACC ACTGACAACA 
     1801 CTCTTGAGCG TTCCTTTTTC ATCCGAATGA AATCTACTCT GACCAAACGC GGTGTGCACA 
     1861 TCAAATCATC AGGATATAAG GTGATTCACA TAACAGGCCG GCTACGCCTG AGAGTGTCGC 
     1921 TGTCCCACGG GAGGACCGTC CCCAGCCAAA TCATGGGTCT CGTGGTTGTT GCGCATGCCT 
     1981 TGCCTCCCCC TACGATCAAT GAAGTCAGAA TTGACTGCCA TATGTTCGTC ACTCGAGTAA 
     2041 ATATGGACCT CAATATCATT TACTGTGAAA ATAGGATTAG TGATTATATG GATCTGACCC 
     2101 CTGTAGATAT CGTAGGGAAG AGATGCTACC ACTTCATCCA TGCTGAAGAC GTGGAGGGCA 
     2161 TCAGGCACAG TCACTTGGAC TTGCTGAATA AGGGTCAGTG TGTGACAAAG TACTATCGCT 
     2221 GGATGCAGAA GAACGGAGGA TATATTTGGA TACAGTCCAG TGCCACCATA GCTATTAATG 
     2281 CCAAGAATGC AAATGAAAAG AATATCATCT GGGTGAATTA CCTTCTTAGC AATCCTGAGT 
     2341 ACAAGGACAC ACCCATGGAC ATCGCACAGC TCCCCCATCT GCCGGAGAAA ACTTCCGAAT 
     2401 CCTCGGAGAC ATCCGACTCT GAGTCAGACT CTAAAGACAC CTCAGGTATT ACAGAGGACA 
     2461 ACGAGAACTC CAAGTCCGAC GAGAAGGGGA ACCAGTCCGA GAACAGCGAA GACCCGGAGC 
     2521 CCGACCGGAA GAAGTCGGGC AACGCGTGTG ACAACGACAT GAACTGCAAC GACGACGGCC 
     2581 ACAGCTCCAG TAACCCGGAC AGCCGCGACA GCGACGACAG CTTCGAGCAC TCGGACTTTG 
     2641 AGAACCCCAA GGCGGGCGAG GACGGCTTCG GTGCTCTGGG CGCGATGCAG ATCAAGGTGG 
     2701 AGCGCTACGT GGAGAGCGAG TCGGACCTGC GGCTGCAGAA CTGCGAGTCA CTCACGTCCG 
     2761 ACAGCGCCAA GGACTCGGAC AGCGCAGGCG AGGCGGGCGC GCAGGCCTCC AGCAAGCACC 
     2821 AGAAGCGCAA GAAAAGGCGG AAACGGCAAA AGGGCGGCAG CGCCAGCCGC CGGCGCCTGT 
     2881 CCAGCGCGTC GAGCCCAGGC GGCCTGGACG CGGGCCTGGT GGAGCCCCCG CGGCTGCTGT 
     2941 CCTCCCCCAA CAGTGCCTCG GTGCTCAAGA TCAAGACGGA GATCTCAGAA CCCATCAATT 
     3001 TCGACAATGA CAGCAGCATC TGGAACTACC CGCCCAACCG GGAGATCTCC AGGAACGAGT 
     3061 CCCCCTACAG CATGACCAAG CCCCCCAGCT CTGAGCACTT CCCGTCCCCG CAGGGCGGCG 
     3121 GCGGTGGGGG TGGCGGTGGC GGGGGGCTGC ACGTGGCCAT TCCCGACTCG GTCCTCACCC 
     3181 CGCCCGGCGC CGACGGCGCG GCCGCCCGCA AGACTCAGTT CGGCGCCTCG GCCACCGCGG 
     3241 CCCTGGCCCC CGTCGCCTCC GACCCGCTGT CACCCCCGCT CTCGGCGTCC CCGCGGGACA 
     3301 AGCACCCCGG GAACGGCGGC GGGGGCGGGG GCGGGGGCGG CGGCGCGGGG GGCGGCGGCC 
     3361 CCAGCGCGTC CAACTCCTTG CTGTACACTG GGGACCTGGA GGCGCTGCAG AGGTTGCAGG 
     3421 CGGGCAACGT CGTGCTCCCG CTGGTGCACA GGGTGACCGG GACCCTGGCC GCCACCAGCA 
     3481 CGGCCGCGCA GAGGGTCTAC ACCACGGGCA CCATCCGCTA CGCGCCCGCC GAGGTGACCC 
     3541 TGGCCATGCA GAGCAACCTG CTGCCCAACG CGCACGCTGT TAACTTCGTG GACGTTAACA 
     3601 GCCCCGGCTT TGGCCTCGAC CCCAAGACGC CCATGGAGAT GCTCTACCAC CACGTGCACC 
     3661 GGCTCAACAT GTCAGGACCG TTCGGCGGCG CAGTGAGCGC AGCTAGCCTG ACGCAGATGC 
     3721 CCGCCGGCAA CGTGTTCACC ACGGCCGAGG GACTCTTCTC CACGCTGCCC TTCCCCGTCT 
     3781 ACAGCAACGG CATCCACGCG GCACAGACTC TGGAGCGCAA GGAGGACAGC GGACCGACGC 
     3841 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3901 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3961 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4021 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4081 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4141 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4201 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4261 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4321 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4381 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4441 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4501 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4561 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4621 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4681 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4741 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4801 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4861 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4921 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4981 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5041 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5101 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5161 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5221 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5281 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5341 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5401 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5461 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5521 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5581 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5641 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5701 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5761 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5821 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5881 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5941 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6001 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6061 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6121 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6181 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6241 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6301 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6361 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6421 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6481 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6541 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6601 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6661 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6721 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6781 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6841 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6901 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6961 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7021 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7081 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7141 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7201 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7261 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7321 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7381 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7441 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7501 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7561 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7621 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7681 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7741 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7801 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7861 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7921 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7981 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8041 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8101 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8161 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8221 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8281 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8341 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8401 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8461 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8521 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8581 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8641 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8701 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8761 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8821 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8881 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8941 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9001 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9061 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9121 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9181 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9241 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9301 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9361 CGAATTTT
//