LOCUS       RG226280                9449 bp    DNA     circular  SYN 
DEFINITION  TBC1D2B (tGFP-tagged) - Human TBC1 domain family, member 2B (TBC1D2B), transcript variant 1
ACCESSION   NM_144572
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3930..4634 
                     /label="GFP"  
     misc_feature    4681..5266 
                     /label="PolyA signal"  
     misc_feature    7358..8003 
                     /label="ColE1"  
     misc_feature    5720..6514 
                     /label="Neo"  
     misc_feature    5351..5653 
                     /label="SV40 ori"  
     misc_feature    8018..8878 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3909..3915 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3909 
                     /label="TBC1D2B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGGGGCC GGAGCCCGGG CGGAGGAGGG CGGCGGCGGC GGCGAGGGCG 
     1081 CGGCGCAGGG GGCGGCCGCG GAGCCCGGGG CGGGTCCGGC GCGGGAGCCA GCGCGGCTGT 
     1141 GTGGCTATCT GCAGAAGCTG TCGGGCAAGG GCCCCCTGCG TGGCTACCGC AGCCGCTGGT 
     1201 TCGTGTTCGA CGCGCGCCGC TGCTACCTTT ACTATTTCAA GAGTCCGCAG GACGCGCTGC 
     1261 CCCTCGGCCA CTTGGACATC GCGGACGCCT GCTTCAGCTA CCAGGGCCCC GACGAGGCGG 
     1321 CGGAGCCGGG CACGGAGCCG CCCGCGCACT TCCAGGTGCA CAGCGCGGGA GCCGTCACGG 
     1381 TGCTCAAGGC TCCCAATCGT CAACTCATGA CTTACTGGTT ACAGGAGCTT CAGCAGAAGA 
     1441 GATGGGAATA TTGTAACAGT CTTGACATGG TCAAGTGGGA CAGCAGGACC TCTCCAACTC 
     1501 CCGGGGATTT TCCTAAGGGT CTTGTAGCCA GAGATAACAC TGATTTAATT TACCCACACC 
     1561 CAAATGCTTC TGCAGAAAAA GCCAGAAATG TCCTAGCTGT GGAGACTGTG CCTGGAGAGC 
     1621 TGGTGGGAGA ACAAGCTGCA AATCAGCCCG CCCCAGGGCA TCCAAATTCC ATTAATTTTT 
     1681 ACTCTTTGAA ACAGTGGGGC AATGAGCTCA AGAATTCGAT GTCTTCTTTC CGTCCTGGGA 
     1741 GAGGACATAA TGATAGTCGG AGGACTGTGT TTTATACCAA TGAAGAGTGG GAACTTTTAG 
     1801 ACCCAACCCC TAAGGACCTA GAGGAGTCCA TAGTACAGGA AGAAAAGAAG AAGCTGACCC 
     1861 CTGAAGGAAA CAAAGGAGTA ACTGGCTCAG GATTCCCCTT TGATTTTGGA CGTAACCCCT 
     1921 ACAAAGGAAA GCGCCCTTTG AAAGACATAA TTGGGTCGTA CAAAAATCGT CACAGCAGTG 
     1981 GTGACCCTTC AAGTGAAGGC ACATCAGGCA GTGGCAGCGT CAGCATCAGG AAGCCGGCCT 
     2041 CCGAAATGCA ACTGCAGGTC CAGAGCCAGC AGGAAGAGCT GGAACAGTTA AAGAAAGACC 
     2101 TGTCCAGTCA GAAGGAGCTT GTTCGACTGC TCCAGCAGAC AGTCCGGTCA TCCCAGTATG 
     2161 ACAAGTATTT CACAAGCAGC CGGCTCTGTG AGGGGGTCCC AAAGGACACG CTCGAGCTTC 
     2221 TGCACCAAAA GGATGATCAG ATTCTGGGCC TTACCAGCCA GCTGGAGAGG TTCAGCTTGG 
     2281 AGAAGGAGAG TCTTCAGCAG GAAGTAAGGA CGCTGAAGAG CAAAGTGGGC GAGCTCAACG 
     2341 AGCAGCTGGG AATGCTCATG GAGACCATCC AAGCCAAGGA CGAGGTCATC ATCAAGCTCA 
     2401 GCGAGGGCGA GGGCAACGGG CCTCCTCCCA CCGTGGCGCC CAGCTCCCCT TCGGTTGTGC 
     2461 CTGTTGCCAG GGACCAGCTG GAACTGGACA GGCTGAAAGA TAATCTACAG GGGTACAAAA 
     2521 CCCAAAACAA ATTTCTAAAT AAGGAGATTT TGGAACTCTC AGCTCTACGA AGAAATGCAG 
     2581 AAAGGAGAGA GAGGGATCTG ATGGCAAAGT ATTCTAGCCT GGAAGCCAAG CTCTGCCAGA 
     2641 TAGAAAGTAA ATACCTGATA TTGCTCCAAG AAATGAAGAC ACCAGTGTGC TCAGAAGACC 
     2701 AGGGGCCCAC CCGGGAGGTC ATAGCCCAGT TGCTGGAGGA TGCTCTGCAG GTTGAGAGCC 
     2761 AAGAGCAGCC GGAGCAAGCA TTTGTTAAAC CTCATCTTGT CAGTGAATAT GATATTTATG 
     2821 GGTTCAGGAC TGTACCTGAG GATGATGAGG AAGAGAAATT GGTTGCCAAG GTCCGCGCGT 
     2881 TGGATCTGAA GACTCTCTAC CTCACAGAAA ACCAGGAAGT CTCCACTGGG GTCAAGTGGG 
     2941 AAAACTATTT TGCAAGTACA GTGAACAGGG AGATGATGTG CTCTCCAGAG TTAAAAAACC 
     3001 TCATCCGTGC GGGCATTCCC CACGAGCACC GTTCCAAGGT GTGGAAGTGG TGTGTGGACC 
     3061 GTCACACCAG GAAGTTCAAG GACAACACTG AGCCTGGCCA CTTCCAGACC TTGCTGCAGA 
     3121 AGGCGCTGGA GAAACAGAAC CCAGCCTCCA AGCAGATTGA GCTGGACTTG CTGCGAACTC 
     3181 TGCCCAACAA CAAACATTAC TCCTGCCCCA CCTCAGAAGG CATACAGAAG TTACGCAATG 
     3241 TCCTCCTCGC CTTCTCCTGG CGGAATCCAG ATATCGGCTA CTGTCAAGGC CTAAACAGGT 
     3301 TGGTGGCAGT GGCCCTCCTG TACCTGGAAC AAGAAGATGC TTTCTGGTGT CTCGTTACCA 
     3361 TAGTGGAAGT TTTCATGCCT CGAGACTATT ATACAAAGAC TCTTTTAGGA TCCCAGGTGG 
     3421 ACCAGCGGGT GTTCAGAGAC CTTATGAGTG AGAAGCTGCC TCGGTTGCAT GGCCACTTTG 
     3481 AACAGTACAA AGTCGACTAC ACTCTCATCA CTTTCAACTG GTTTCTGGTG GTATTTGTGG 
     3541 ATAGTGTCGT TAGTGACATC CTCTTTAAAA TATGGGACTC TTTCCTTTAT GAAGGACCAA 
     3601 AGGTTATTTT CCGTTTTGCT CTGGCACTTT TTAAGTACAA GGAAGAGGAG ATTTTGAAAT 
     3661 TGCAAGATTC GATGTCTATA TTTAAGTATC TCCGCTACTT CACTCGCACT ATCCTTGATG 
     3721 CTAGGAAGCT GATCAGTATC TCCTTTGGGG ACCTGAACCC TTTCCCCCTA CGCCAGATCC 
     3781 GGAACCGACG CGCCTACCAC TTGGAGAAAG TCCGGCTGGA GCTGACCGAG CTGGAGGCCA 
     3841 TCCGTGAGGA CTTCCTGCGT GAGCGGGACA CCAGCCCTGA CAAGGGTGAG CTGGTCAGTG 
     3901 ACGAGGAGGA GGATACCACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3961 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     4021 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     4081 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     4141 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     4201 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     4261 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4321 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4381 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4441 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4501 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4561 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4621 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4681 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4741 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4801 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4861 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4921 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4981 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5041 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5101 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5161 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5221 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5281 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5341 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5401 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5461 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5521 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5581 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5641 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5701 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5761 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5821 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5881 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5941 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     6001 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     6061 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     6121 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     6181 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     6241 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6301 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6361 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6421 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6481 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6541 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6601 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6661 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6721 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6781 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6841 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6901 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6961 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     7021 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     7081 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     7141 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     7201 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7261 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7321 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7381 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7441 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7501 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7561 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7621 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7681 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7741 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7801 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7861 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7921 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7981 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     8041 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     8101 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     8161 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     8221 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     8281 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8341 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8401 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8461 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8521 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8581 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8641 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8701 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8761 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8821 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8881 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8941 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     9001 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     9061 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     9121 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     9181 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     9241 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9301 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9361 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9421 TGATTTAACA AAAATTTAAC GCGAATTTT
//