LOCUS       RG226257                9296 bp    DNA     circular  SYN 
DEFINITION  SLFN14 (tGFP-tagged) - Human schlafen family member 14 (SLFN14)
ACCESSION   NM_001129820
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3777..4481 
                     /label="GFP"  
     misc_feature    4528..5113 
                     /label="PolyA signal"  
     misc_feature    7205..7850 
                     /label="ColE1"  
     misc_feature    5567..6361 
                     /label="Neo"  
     misc_feature    5198..5500 
                     /label="SV40 ori"  
     misc_feature    7865..8725 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3756..3762 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3756 
                     /label="SLFN14"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGTCTC AAGACTGATA CTGAAATGCC GTATCCTGAG GTAATAGTAG 
     1081 ATGTGGGCAG AGTGATTTTT GGAGAAGAAA ACAGGAAGAA GATGACCAAC AGCTGTTTGA 
     1141 AAAGATCTGA GAATTCTAGA ATTATCCGGG CTATATGTGC ACTGTTAAAT TCTGGAGGTG 
     1201 GTGTGATCAA AGCAGAGATT GATGATAAAA CCTATAGTTA CCAATGCCAT GGGCTGGGAC 
     1261 AGGATTTGGA AACTTCTTTT CAAAAGCTCC TTCCTTCAGG TTCACAGAAA TACCTTGACT 
     1321 ACATGCAGCA GGGGCACAAT CTCCTGATTT TTGTGAAGTC ATGGAGCCCA GATGTTTTCA 
     1381 GCCTTCCACT AAGGATTTGC AGCTTGCGCT CCAATTTGTA TCGGAGAGAT GTGACTTCTG 
     1441 CTATCAACTT GAGTGCTAGC AGTGCCCTGG AGCTTCTCAG AGAGAAGGGG TTTAGAGCCC 
     1501 AAAGAGGAAG ACCAAGGGTG AAGAAGTTGC ATCCTCAGCA GGTTCTCAAT AGATGCATTC 
     1561 AGGAAGAGGA AGATATGAGG ATATTGGCCT CAGAATTTTT TAAAAAGGAC AAACTCATGT 
     1621 ATAAGGAGAA ACTCAACTTT ACTGAGTCAA CACATGTTGA ATTTAAAAGG TTCACCACCA 
     1681 AAAAAGTCAT ACCTCGGATT AAGGAAATGC TGCCTCATTA TGTTTCTGCA TTTGCCAACA 
     1741 CTCAAGGGGG ATATGTCCTC ATTGGGGTGG ATGATAAGAG CAAAGAAGTG GTTGGATGTA 
     1801 AGTGGGAAAA AGTGAATCCT GACTTACTAA AAAAAGAAAT CGAAAACTGC ATAGAAAAAT 
     1861 TGCCTACATT CCACTTCTGC TGTGAGAAGC CAAAGGTAAA TTTCACTACA AAAATCCTGA 
     1921 ATGTGTACCA AAAAGATGTC CTGGATGGTT ATGTCTGTGT GATTCAAGTG GAGCCCTTCT 
     1981 GTTGCGTGGT GTTTGCAGAG GCCCCAGATT CCTGGATCAT GAAAGACAAT TCTGTCACAC 
     2041 GGCTGACAGC TGAGCAGTGG GTGGTCATGA TGCTGGATAC TCAGTCAGCT CCTCCCAGTT 
     2101 TGGTCACAGA CTACAACTCT TGCCTGATTT CATCAGCTTC ATCTGCACGA AAAAGTCCCG 
     2161 GATATCCCAT AAAAGTCCAC AAATTTAAGG AGGCTCTGCA ACGACATTTG TTTCCAGTGA 
     2221 CACAGGAAGA GGTACAATTT AAACCAGAAT CCCTCTGTAA GAAGCTGTTC TCAGATCATA 
     2281 AAGAACTGGA GGGTTTAATG AAGACCCTGA TACATCCTTG TTCTCAGGGG ATTGTGATAT 
     2341 TTTCTAGAAG CTGGGCTGGT GATGTTGGCT TCAGGAAAGA ACAGAATGTC CTGTGTGATG 
     2401 CTCTCCTGAT AGCAGTTAAC AGCCCCGTGG TACTCTATAC AATCTTAATA GACCCCAATT 
     2461 GGCCTGGAGG ACTTGAATAT GCCCGAAACA CAGCTCATCA GTTAAAGCAG AAACTGCAAA 
     2521 CTGTTGGTGG TTACACAGGG AAAGTGTGCA TCATTCCAAG GCTGATACAC CTGAGCAGCA 
     2581 CACAGAGTAG ACCTGGTGAG ATCCCCCTGC GTTACCCCAG GTCCTACAGG CTTGCTGATG 
     2641 AGGAGGAAAT GGAAGACTTG CTGCAGGCTC TGGTGGTGGT CTCCCTGTCC TCCAGATCTC 
     2701 TTCTGAGTGA CCAGATGGGC TGTGAATTTT TCAACTTGCT CATAATGGAG CAGAGCCAGT 
     2761 TGCTTTCTGA GAGTCTTCAG AAGACACGTG AATTATTCAT CTACTGCTTT CCAGGAGTCA 
     2821 GGAAGACAGC CCTAGCCATA AAGATCATGG AGAAAATTAA GGACTTGTTT CACTGCAAAC 
     2881 CAAAAGAGAT CCTCTATGTT TGTGAAAGCG ACTCCCTAAA GGATTTTGTG ACCCAACAAA 
     2941 CCACCTGCCA AGCTGTGACC AGGAAAACTT TCATGCAAGG GGAGTTTCTA AAGATTAAAC 
     3001 ACATAGTGAT GGATGAGACT GAGAATTTCT GCAGCAAATA TGGCAATTGG TACATGAAGG 
     3061 CTAAGAACAT CACCCATCCA AAGGCGAAGG GGACTGGAAG TGAAAACCTT CACCATGGGA 
     3121 TTCTCTGGCT TTTTCTTGAC CCTTTTCAAA TCCATCACGC AGATGTCAAT GGCCTTCCCC 
     3181 CTCCATCTGC TCAGTTTCCT CGAAAAACAA TCACCAGTGG GATCCACTGT GCTCTGGAAA 
     3241 TAGCGAAGGT TATGAAAGAA GAAATGAAGA GGATCAAAGA AAATCCTCCC TCCAACATGT 
     3301 CTCCAGACAC ATTAGCATTG TTCAGCGAGA CTGCCTATGA GGAAGCAACG TGTGCCCAGG 
     3361 CTCTGCCTGG GGTGTGTGAG ACAAAGACTA ATCTGACAAC AGAACAAATA GCTAACTATG 
     3421 TGGCAAGAAA ATGTCACAGC CTGTTCCAGT GTGGCTATCT GCCCAAAGAT ATAGCAATTC 
     3481 TGTGCAGGAG AGGGGAGGAC AGAGGACGCT ATAGGCTTGC ACTACTCAAA GCAATGGAAT 
     3541 TAATTGAGAC CCACAGACCA TCAGAGGTTG TGTTTAGCCC GGCCACCGGT GTTTGGGGAA 
     3601 GTCACATTGT TTTAGACAGT ATTCAGCAAT TTTCAGGCCT GGAGAGGACT GTCGTGTTTG 
     3661 GGCTTAGTCC AGAATGTGAC CAGTCAGAGG AATTTCATAA GCTCTGCTTT GCTTCAAGAG 
     3721 CCATTAAACA CCTCTACCTG CTTTATGAAA AGAGGGCAGC CTACACGCGT ACGCGGCCGC 
     3781 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3841 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3901 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3961 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     4021 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     4081 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     4141 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4201 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4261 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4321 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4381 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4441 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5221 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5281 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5341 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5401 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5461 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5521 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5581 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5641 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5701 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5761 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5821 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5881 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5941 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     6001 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     6061 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     6121 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6181 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6241 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6301 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6361 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6421 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6481 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6541 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6601 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6661 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6721 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6781 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6841 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6901 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6961 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     7021 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     7081 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     7141 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7201 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7261 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7321 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7381 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7441 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7501 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7561 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7621 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7681 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7741 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7801 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7861 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7921 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7981 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     8041 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     8101 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     8161 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8221 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8281 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8341 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8401 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8461 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8521 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8581 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8641 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8701 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8761 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8821 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8881 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8941 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     9001 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     9061 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     9121 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9181 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9241 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//