LOCUS       RG226245                9257 bp    DNA     circular  SYN 
DEFINITION  PRRT4 (tGFP-tagged) - Human proline-rich transmembrane protein 4 (PRRT4), transcript variant 2
ACCESSION   NM_001114726
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3738..4442 
                     /label="GFP"  
     misc_feature    4489..5074 
                     /label="PolyA signal"  
     misc_feature    7166..7811 
                     /label="ColE1"  
     misc_feature    5528..6322 
                     /label="Neo"  
     misc_feature    5159..5461 
                     /label="SV40 ori"  
     misc_feature    7826..8686 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3717..3723 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3717 
                     /label="PRRT4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGGCAT GGCTGTCTAG GGCTGGGACT GTTCTGCTGC GTCCTGTTTG 
     1081 CTGCTACTGT GGGCCCCCAG CCCACCCCCT CCATCCCAGG TGCCCCTGCC ACCACTTTGA 
     1141 CCCCCGTACC TCAAAGTGAG GCCTCTATGC TGTCTCTCAA CCTGGGACTT AACTTCAAAT 
     1201 TCCATCTTCG GGGACCTGCT GCTGTCTGGG GGAGCCCAGT CACAGAGACC CAGCCACTCT 
     1261 CTCTTGGGCC AGGCCAGGAG CCAGGGGAAG AGGTGGCCAG TGGGCTGAGG ACTGACCCCC 
     1321 TTTGGGAATT GCTGGTGGGC TCCTCAGGGA ACTCTCTCAC TGAGTGGGGC TCCACCGAAG 
     1381 GTGGCTCAAA GCCCCGGGCC TCCTCCCTGC TTCCGGAGTC CACATCCCGG CGCTCTGGGC 
     1441 CCAGCGATGG GCCCACTGCC CCCTATCAGC CCAGGAGGAG CACTGTGACC TGGGACACTG 
     1501 CTCTGATGGT GACAGCACTT CCATCCAGTG CTCCCAGGCC CCACCAGAGC GAGCTGGAGC 
     1561 TGAAGTTTGA CATGGCACTG AGAGCAGGTG CAGCCCCCAC GCTTGGGCAT CGAACGCTGC 
     1621 CCCTGCTGCC CAGCCTGCGG GCCAGCCTGG CAGAGATTGC TGGGCGCCTG GGACCCTTTG 
     1681 GATTCTTTGG CACTACTCTG TCCCCACTCC GGAACTTCTC CGGCCTGAGC CCCCCAGGTG 
     1741 AAACTACATC CACAAGCTCT GCCTCTGGAG TTTCGGGTTC TCTGGGGTTC CTTGGTACCA 
     1801 CTCTGTCCCT GCCCCCATAC TCCCTGGAGA GGAAGCTCTC CAGCCCAAGT CCTCTGGACC 
     1861 CAGCTGCTTC CCTAAGTTTT GCCTCGATTG CAACAACATC ATTAGACCCC ACAGTCCCCA 
     1921 TCTCTGGCCC AGATGACCTC TCTCCTCCCG CCAGCCTCGG GAACCCTTCG GGGCAGCCAG 
     1981 AGTGTGGGCC AGGGTCCTGC AGCGTGGGAG AATTGCCTGA ACGCGAGGGG CAGCCTCCCG 
     2041 AGGCGCCGAG GCCCCTCTTT TTCCTGACCC TGGAGGCCGA CTGGGCAGAG GCCAGGGCTC 
     2101 GCTGGGGGCT GGCCTGGGAG GCCCACGTGT ACGGGGTAGG CGCGCTCTTC GGCCTGGTTG 
     2161 CCTTGCTGGC GCTGCTGGCT CTGGCCCTCT TGCCCTGGCG GTGCCCGCCC GGCGCCCCCT 
     2221 GCCTGGCGCT GCTGGACCTG CTGCTGCTGT CGGCCGGGAC CACGCGGGCC TTCCCGCTCT 
     2281 TCTACGACGC CTATGGGCAC AGGGATCGAC TGCCCGCGCT CGCCTGGCTG CTGCTGCAGG 
     2341 ACCTTCCGCT GCCCTGCTTG GCTGCCGGCC TGGGGCTGGC CTGCCTGCTG CTGGCCCGGC 
     2401 CGCGCCCGCC GCGGTGCCCC ACCGGCCTGG CTGCGCTGCT GCTCCTGGGG CTGGGGCTGG 
     2461 CGGCCGCCGC CGCCCTCGGG AGCGCCGCGC ATCGCCCGCT GCGGCCCCTG CGGCTCGCCT 
     2521 CGCGCGGGCT GCACGCCTTT CTCGCCGCTT TCCTTTCCGG GCTGCTGCTG GCGCTCTCCT 
     2581 GCTGGGGCGG GCGGCGGCGG CGGGCCGGGG CGCCCCTGGG AGGGTCGGGC TTCAAGGGCG 
     2641 CCACACCCCT GCCGCAGGGG CGCAGCCCCT TCGCCCCTCG GGAGTCCTGG CGGCGCGCGG 
     2701 CGCGCACGGC CCCGGTGGCG GGCACCTTCG GGCTGCTGAG CGGAGCCCTG CAGGGCTATG 
     2761 AGGTGCTGCA CGCCCTGGGC TACGGCGGCC AATCCGGCCT GGAAGGGCCC TGGCCCTGGT 
     2821 GGGCCTTCCA GCTAGGCCTG CGCCTGGGCG AGGTGGGCGT CGCGCTCCCG TTGGCGCTGC 
     2881 TGGGCCTCTA CCCGGCGCTC TGCAGTCCTC GCGTGCCGCC GCGCTGCTGG GCCAAGCTCT 
     2941 TCCGCTTGTC CCCGGGCCAC GCGGCTCCGC TGCTGCCGGG AGGCTGGGTC ACTGGGCCCC 
     3001 CAGACAAGGA GCCCCTGGGG AGCGCCATCG CTCGCGGGGA CGCGGAGCTG CTGCAGCTGT 
     3061 GCGCGCTGGC AGGGCCAGGC CCAGACCTCC TACTCCAGGG CGGAGGTTGC CGGGGCTTCG 
     3121 AAGGCGCGGC GGCCAACCCG GCCCCGTCCC CGGCTTCCTC TCCCTGCAGC GATTACACCG 
     3181 TGGACTTCCG CCCGCCCTCC CCAATCAACC TGCGACGCAG CATCGAGGAG GCCCTCTGCA 
     3241 GCGAGGCCCT GCTTGCGCCT GGCCTCTTCC AGGGCCCTGC CTTCGAGGAC GCTCTGCCTG 
     3301 GGCTCGGACT CTACCGCACC GCCTCGCTGG GGACCGGGGG CAGGGCCAGT GAGAGATCAG 
     3361 GGGAGGCCTC TGGCCCCGCT GCGCCCCCGG AGCTCCCCTC CCCTGGGGCT TGGCCCGCAG 
     3421 GCAGCAGCGT CTCATCTGGC TCGTTCTGCG GACTCTCGCG GGACAGCTCG TCCATGCTGC 
     3481 TGTGTTCCAG CCCCGACAGG CCCCCGCGCT GCCCTCTGGT CTGCGTCCTC AGTCCCCCGC 
     3541 GGCCCTCAGG AAGCAGCCCC AGCCTCCCGG CCTCAGGATC CTACCAGGCC CTGTCCCCAC 
     3601 CCTCTCGCGA CTCCCCAGAG CCTGCTTCTG AGCTGCAGGC CGAGGAGGCC TTGCTGCAGG 
     3661 AGCAGTTCCT GGACGCCTGC CGACAGATCG ACGAGCTGAG CGTGGGCAGC GACACCATAG 
     3721 ACCTGACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3781 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3841 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3901 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     3961 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4021 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4081 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4141 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4201 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4261 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4321 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4381 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4441 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4501 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4561 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4621 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4681 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4741 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4801 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4861 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4921 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4981 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5041 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5101 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5161 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5221 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5281 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5341 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5401 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5461 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5521 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5581 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5641 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5701 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5761 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5821 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     5881 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     5941 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6001 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6061 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6121 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6181 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6241 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6301 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6361 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6421 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6481 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6541 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6601 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6661 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6721 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6781 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6841 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6901 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     6961 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7021 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7081 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7141 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7201 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7261 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7321 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7381 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7441 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7501 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7561 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7621 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7681 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7741 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7801 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7861 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7921 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     7981 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8041 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8101 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8161 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8221 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8281 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8341 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8401 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8461 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8521 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8581 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8641 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8701 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8761 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8821 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     8881 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     8941 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9001 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9061 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9121 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9181 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9241 AATTTAACGC GAATTTT
//