LOCUS       RG224460                9164 bp    DNA     circular  SYN 
DEFINITION  IGFN1 (tGFP-tagged) - Human immunoglobulin-like and fibronectin type III domain containing 1 (IGFN1)
ACCESSION   NM_178275
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3645..4349 
                     /label="GFP"  
     misc_feature    4396..4981 
                     /label="PolyA signal"  
     misc_feature    7073..7718 
                     /label="ColE1"  
     misc_feature    5435..6229 
                     /label="Neo"  
     misc_feature    5066..5368 
                     /label="SV40 ori"  
     misc_feature    7733..8593 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3624..3630 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3624 
                     /label="IGFN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGTGGCAG CCTATGGGAG AGAACTGGGG GTGCCTGGAG GAGATGCTGA 
     1081 ATGAAGATCA GAGCCGGGAG CCCCCTGGTC ACCTTGGTAG CAGGAGAAGT GGCAAAGACG 
     1141 GCAGGTTGGA CATCTATGGA GAGAGGAGAG ATGCTACCCG GAGTTCCACA TCCAGATACA 
     1201 AGCCTGGCAC TGGCAGTTTC TCCAAGGATG CCCAAGGCCC CATGGGCCAC TTCTCCCAGG 
     1261 GCCTGGCTGA CATGGAAGTG CAGCCGGGGG AGGCCGCCAC ACTCTCCTGT ACCCTCACCA 
     1321 GTGACCTGGG ACCTGGCACC TGGTTTAAGG ATGGCGTCAA GCTCACCACC CAGGATGGAG 
     1381 TCATCTTTAA GCAAGACGGT CTCGTGCACA GCCTCTTCAT CACGCATGTG CAGGGGACCC 
     1441 AAGCTGGGAG GTACACCTTT GTAGCTGGTG ACCAGCAGAG CGAGGCCACC CTGACCGTCC 
     1501 AGGATTCCCC TACCATTGCT CCAGATGTGA CAGAGAAACT GAGAGAGCCA CTGGTGGTCA 
     1561 AGGCTGGGAA GCCGGTGATA GTGAAGATCC CCTTCCAGAG CCACCTCCCC ATTCAGGCTG 
     1621 CCTGGAGGAA GGACGGGGCT GAGGTGGTGG GCAGCAGTGA CAGGGAGGCC CAGGTGGACC 
     1681 TGGGGGATGG CTACACGCGG CTGTGCCTCC CCAGCGCAGG CAGGAAGGAC TGTGGCCAGT 
     1741 ACAGCGTGAC ACTGAGGAGT GAGGGAGGCT CTGTGCAGGC CGAGCTCACT CTGCAAGTCA 
     1801 TAGACAAGCC TGATCCCCCA CAAGGCCCCA TGGAGGTTCA GGATTGCCAT AGGGCTGGCG 
     1861 TCTGCCTCCG CTGGCGGCCC CCAAGGGACA ATGGGGGCCG GACTGTAGAG TGCTACGTGG 
     1921 TGGAGAGACG GCAGGCTGGC AGGAGCACTT GGCTGAAGGT GGGCGAGGCC CCCGCTGACA 
     1981 GCACCACCTT CACGGATGCC CATGTGGAGC CAGGCAGGAA GTATACCTTC CGAGTGCGGG 
     2041 CTGTGACCTC AGAGGGGGCT GGCGAGGCCC TGGAGTCTGA GGAGATATTG GTGGCTCCTG 
     2101 AGGCTCTCCC CAAGGCCCCT TCCGCGCCAG CCATCCTGTC GGCCTCCAGC CAGGGCATCA 
     2161 CACTGACATG GACAGCACCT CGGGGCCCCG GCAGCGCCCA CATCCTGGGC TACCTGATCG 
     2221 AGAGGCGTAA GAAGGGGAGC AACACCTGGA CGGCAGTGAA CGACCAGCCG GTGCCTGAGA 
     2281 GGAGGTGGAC GGTGGCGGAC GTGCGGCAGG GCTGTCAGTA TGAGTTCCGG GTCACAGCTG 
     2341 TGGCTCCCTC AGGTCCCGGA GAGCCTGGAC CTCCATCGGA TGCCGTCTTT GCTCGGGACC 
     2401 CCATGAGACC CCCTGGGCTG GTGAGGAATC TCCAAGTCAC AGACAGATCG AACACCAGCA 
     2461 TCACTCTGAG CTGGGCTGGG CCAGACACCC AGGAAGGGGA TGAAGCCCAG GGGTATGTGG 
     2521 TGGAGCTGTG CAGCTCAGAC AGTCTCCAGT GGCTCCCGTG CCATGTAGGC ACCGTGCCAG 
     2581 TCACCACCTA CACGGCCAAG GGGCTTCGGC CTGGAGAGGG CTACTTCGTG CGGGTGACAG 
     2641 CAGTTAATGA AGGAGGCCAG AGCCAGCCCA GTGCCCTGGA CACATTAGTG CAAGCCATGC 
     2701 CTGTTACTGT CTGTCCCAAG TTCCTCGTGG ACTCCAGCAC CAAGGACTTG CTGACAGTCA 
     2761 AGGTCGGGGA CACAGTTCGT GTGCCCGTCT CCTTTGAAGC CATGCCCATG CCTGAGGTGA 
     2821 CCTGGCTGAA GGATGGCTTG CCCTTGCCCA AAAGAAGTGT GACTGTCACT AAGGATGGCC 
     2881 TCACCCAGCT TCTGATCCCT GTGGCTGGAC TCTCAGACAG TGGTCTCTAC ACTGTGGTGC 
     2941 TGAGGACCCT GCAGGGGAAG GAGGTTGCCC ACAGCTTCCG TATCAGGGTG GCAGCATGCC 
     3001 CGCAGGCCCC TGGGCCCATC CACCTGCAGG AGAACGTGCC TGGGACGGTG ACGGCCGAGT 
     3061 GGGAACCCTC TCCTGACGAG GCCCAGGATG TCCCGCTGCA CTACGCGGTG TTCACACGCT 
     3121 CCTCAGCGCA CGGTCCCTGG CACGAGGCAG CCGACCGCAT CTACACCAAC CGCTTCACCC 
     3181 TCCTGGGCAT CCTCCCCGGC CACGAATACC ACTTCAGGGT GGTGGCCAAG AATGAGCTGG 
     3241 GGGCCAGCAA ACCCTCGGAC ACCAGCCAGC CCTGGTGCAT CCCCCGGCAG CGCGACAGGT 
     3301 TCACAGTGAA GGCTCCGTGC TACCGGGAGC CCGACCTGAG CCAGAAGCCC CGGTTCCTGG 
     3361 TGGGCCTGCG GTCCCACCTG CTGCCCCAGG GCTGCGAGTG CTGCATGAGC TGTGCCGTGC 
     3421 AGGGCTCGCC CCGGCCCCAC GTCACCTGGT TCAAGAATGA CCGCAGCCTG GAAGGAAACC 
     3481 CCGCGGTGTA CAGCACTGAC CTGCTGGGCG TGTGCTCCCT CACCATCCCC AGCGTATCCC 
     3541 CGAAGGACAG CGGGGAGTAC AAGGCTGTGG CTGAGAACAC GCTGGGCCAG GCAGTCAGCA 
     3601 CTGCCACCCT CATTGTCATA GAACCCAGCA CCACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3661 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3721 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3781 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3841 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     3901 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     3961 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4021 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4081 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4141 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4201 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4261 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4321 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4381 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4441 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4501 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4561 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4621 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4681 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4741 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4801 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4861 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4921 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4981 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5041 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5101 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5161 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5221 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5281 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5341 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5401 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5461 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5521 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5581 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5641 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5701 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5761 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5821 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     5881 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     5941 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6001 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6061 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6121 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6181 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6241 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6301 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6361 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6421 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6481 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6541 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6601 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6661 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6721 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6781 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6841 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     6901 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     6961 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7021 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7081 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7141 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7201 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7261 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7321 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7381 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7441 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7501 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7561 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7621 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7681 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7741 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7801 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7861 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     7921 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     7981 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8041 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8101 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8161 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8221 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8281 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8341 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8401 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8461 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8521 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8581 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8641 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8701 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8761 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8821 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     8881 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     8941 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9001 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9061 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9121 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//