LOCUS       RG224384                9398 bp    DNA     circular  SYN 
DEFINITION  ITIH2 (tGFP-tagged) - Human inter-alpha (globulin) inhibitor H2 (ITIH2)
ACCESSION   NM_002216
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3879..4583 
                     /label="GFP"  
     misc_feature    4630..5215 
                     /label="PolyA signal"  
     misc_feature    7307..7952 
                     /label="ColE1"  
     misc_feature    5669..6463 
                     /label="Neo"  
     misc_feature    5300..5602 
                     /label="SV40 ori"  
     misc_feature    7967..8827 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3858..3864 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3858 
                     /label="ITIH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAAGACTC ACGTGCTTTT TCATCTGCTT CTTTCTTTCT GAAGTATCAG 
     1081 GCTTCGAAAT CCCCATAAAT GGACTTTCTG AATTTGTAGA CTATGAAGAT CTTGTGGAAC 
     1141 TGGCCCCAGG CAAATTTCAA TTGGTGGCAG AGAACCGGAG ATATCAGAGA AGCCTTCCAG 
     1201 GAGAATCGGA AGAAATGATG GAAGAGGTTG ATCAAGTAAC TCTTTATAGC TATAAAGTCC 
     1261 AGTCTACTAT TACTTCTCGG ATGGCCACCA CCATGATCCA GAGCAAAGTG GTGAACAATT 
     1321 CCCCGCAGCC TCAGAATGTC GTGTTTGATG TTCAGATCCC CAAAGGAGCA TTCATTTCCA 
     1381 ACTTCTCCAT GACTGTGGAC GGCAAGACAT TTAGGAGCTC TATTAAGGAG AAAACTGTGG 
     1441 GCCGAGCTCT TTATGCACAG GCCAGAGCAA AAGGCAAGAC GGCTGGCTTG GTGAGGAGCA 
     1501 GCGCTCTTGA TATGGAAAAC TTCAGAACGG AAGTAAATGT CCTCCCAGGA GCAAAGGTGC 
     1561 AGTTCGAACT TCACTACCAG GAGGTGAAGT GGAGGAAGCT GGGCTCCTAT GAGCACAGGA 
     1621 TCTATCTGCA ACCTGGACGG CTGGCCAAAC ACTTAGAGGT AGATGTGTGG GTTATCGAAC 
     1681 CACAGGGACT GAGATTTCTT CATGTTCCCG ACACATTTGA AGGCCATTTC GATGGTGTTC 
     1741 CGGTCATTTC TAAAGGACAA CAGAAGGCGC ACGTCTCCTT CAAGCCCACG GTAGCACAGC 
     1801 AGAGAATATG CCCTAACTGC CGGGAGACTG CGGTAGATGG GGAACTGGTG GTGCTGTATG 
     1861 ACGTGAAAAG AGAAGAGAAG GCTGGTGAAC TGGAGGTGTT TAATGGATAT TTTGTCCACT 
     1921 TCTTTGCTCC TGACAACCTG GACCCAATTC CCAAAAACAT CCTCTTTGTC ATCGATGTGA 
     1981 GTGGCTCCAT GTGGGGAGTT AAAATGAAAC AAACTGTGGA AGCAATGAAG ACCATATTGG 
     2041 ATGACCTCAG AGCAGAAGAC CATTTCTCTG TGATTGATTT CAACCAGAAC ATTCGAACTT 
     2101 GGAGAAATGA TTTAATTTCA GCTACAAAAA CACAGGTTGC AGATGCCAAG AGGTATATTG 
     2161 AGAAAATCCA GCCCAGTGGA GGCACAAACA TCAACGAAGC ACTCCTACGG GCAATCTTCA 
     2221 TTTTGAATGA AGCCAATAAC TTGGGACTGT TAGACCCCAA CTCCGTCTCG CTGATCATTT 
     2281 TGGTTTCTGA TGGAGATCCA ACAGTGGGCG AACTAAAACT GTCAAAAATT CAGAAAAACG 
     2341 TTAAGGAGAA CATCCAAGAC AATATCTCCT TGTTCAGTTT GGGCATGGGA TTTGATGTGG 
     2401 ACTATGATTT TTTGAAGAGA CTGTCCAATG AAAACCATGG AATTGCACAA AGGATTTATG 
     2461 GAAACCAGGA CACGTCTTCC CAGCTTAAGA AATTCTACAA CCAGGTCTCC ACTCCATTGC 
     2521 TCCGGAATGT TCAGTTCAAC TATCCCCATA CATCAGTCAC GGACGTCACT CAAAACAATT 
     2581 TCCATAACTA CTTTGGAGGC TCAGAGATTG TGGTGGCAGG AAAATTTGAC CCTGCTAAAT 
     2641 TGGATCAAAT AGAGAGCGTT ATCACGGCGA CTTCGGCTAA CACGCAGTTA GTCTTGGAGA 
     2701 CCCTGGCCCA GATGGACGAC TTGCAGGATT TTCTATCGAA AGACAAGCAT GCAGATCCCG 
     2761 ATTTCACCAG GAAACTGTGG GCCTATCTAA CCATCAACCA ACTGCTAGCT GAACGAAGCC 
     2821 TGGCTCCTAC AGCTGCCGCC AAGAGAAGAA TTACAAGATC GATCCTGCAG ATGTCTCTAG 
     2881 ACCACCACAT TGTGACTCCG CTGACCTCGC TGGTGATCGA GAACGAGGCT GGGGATGAGC 
     2941 GCATGCTGGC GGATGCCCCA CCGCAGGATC CCTCCTGCTG CTCAGGGGCC CTGTATTACG 
     3001 GCAGCAAAGT GGTTCCAGAT TCCACCCCGT CTTGGGCCAA TCCTTCACCA ACGCCCGTGA 
     3061 TCTCCATGCT GGCACAAGGA TCTCAGGTGC TAGAGTCCAC GCCACCCCCA CATGTGATGA 
     3121 GAGTTGAAAA TGACCCACAT TTCATCATTT ATCTACCAAA AAGCCAAAAG AACATTTGTT 
     3181 TCAATATTGA CTCAGAACCT GGAAAAATCC TCAACCTGGT TTCTGACCCA GAATCAGGAA 
     3241 TTGTAGTCAA CGGTCAGCTT GTTGGTGCCA AGAAGCCCAA CAATGGAAAA CTAAGCACCT 
     3301 ATTTTGGAAA ACTGGGATTT TATTTCCAAA GTGAAGACAT AAAAATAGAA ATCAGCACTG 
     3361 AGACCATCAC CCTGAGCCAT GGTTCTAGCA CATTCTCCTT GTCCTGGTCC GACACGGCTC 
     3421 AAGTCACGAA TCAGAGGGTG CAGATCTCAG TGAAGAAAGA AAAAGTGGTA ACTATCACCC 
     3481 TGGATAAAGA GATGTCCTTT TCTGTTTTAC TTCATCGTGT TTGGAAGAAG CATCCCGTCA 
     3541 ATGTTGACTT TCTGGGAATC TACATACCCC CTACAAACAA GTTCTCACCT AAAGCCCACG 
     3601 GACTAATAGG CCAGTTCATG CAGGAACCAA AGATACACAT CTTCAATGAG AGACCAGGAA 
     3661 AGGACCCTGA GAAGCCAGAG GCCAGCATGG AAGTGAAGGG GCAGAAGCTG ATCATCACCA 
     3721 GGGGCTTACA GAAAGACTAC AGAACGGATC TAGTGTTTGG AACGGACGTT ACCTGCTGGT 
     3781 TTGTGCACAA CAGTGGAAAA GGATTCATTG ACGGGCATTA CAAGGATTAC TTCGTGCCTC 
     3841 AGCTCTACAG CTTTCTCAAA CGGCCTACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3901 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3961 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     4021 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     4081 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     4141 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4201 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4261 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4321 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4381 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4441 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4501 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4561 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4621 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4681 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4741 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4801 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4861 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4921 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4981 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5041 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5101 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5161 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5221 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5281 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5341 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5401 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5461 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5521 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5581 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5641 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5701 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5761 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5821 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5881 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5941 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     6001 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     6061 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     6121 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6181 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6241 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6301 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6361 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6421 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6481 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6541 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6601 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6661 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6721 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6781 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6841 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6901 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6961 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     7021 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     7081 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     7141 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7201 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7261 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7321 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7381 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7441 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7501 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7561 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7621 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7681 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7741 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7801 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7861 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7921 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7981 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     8041 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     8101 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     8161 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8221 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8281 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8341 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8401 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8461 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8521 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8581 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8641 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8701 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8761 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8821 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8881 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8941 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     9001 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     9061 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     9121 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9181 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9241 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9301 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9361 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//