LOCUS       RG224312                9356 bp    DNA     circular  SYN 
DEFINITION  PCDHGA8 (tGFP-tagged) - Human protocadherin gamma subfamily A, 8 (PCDHGA8), transcript variant 1
ACCESSION   NM_032088
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3837..4541 
                     /label="GFP"  
     misc_feature    4588..5173 
                     /label="PolyA signal"  
     misc_feature    7265..7910 
                     /label="ColE1"  
     misc_feature    5627..6421 
                     /label="Neo"  
     misc_feature    5258..5560 
                     /label="SV40 ori"  
     misc_feature    7925..8785 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3816..3822 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3816 
                     /label="PCDHGA8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCTCCA CAGAGTCGCC CCAGACGCGG CGAGCTGATC CTGCTGTGCG 
     1081 CGCTGCTGGG AACGCTGTGG GAAATCGGGA GGGGACAGAT TCGCTACTCT GTGCCAGAAG 
     1141 AGACGGACAA AGGCTCCTTC GTGGGTAATA TCTCCAAGGA CCTGGGGCTG GACCCCCGGA 
     1201 AGCTGGCGAA GCACGGAGTC CGTATCGTCT CCAGAGGTAG GACGCAGCTC TTTGCTCTGA 
     1261 ACCCGCGCAG CGGCAGCTTG ATCACCGCGG GCAGGATAGA TCGGGAGGAG CTCTGCGCTC 
     1321 AGAGCCCGCG GTGTCTGATA AATATTAACA CCCTGGTTGA GGATAAAGGA AAACTCTTTG 
     1381 GGGTAGAAAT AGAAATAATT GATATTAACG ATAATAACCC AAAATTCCAG GTCGAAGATC 
     1441 TAGAAGTAAA AATTAACGAA ATCGCGGTTC CTGGAGCACG TTATCCACTC CCAGAAGCTG 
     1501 TTGACCCGGA TGTGGGCGTG AACTCCCTCC AGAGCTACCA GCTCAGCCCC AATCACCACT 
     1561 TCTCCCTGGA CGTGCAGACT GGAGACAATG GAGCCATAAA CCCAGAGCTG GTGCTGGAGC 
     1621 GCGCCCTGGA CAGGGAGGAA GAGGCTGCTC ACCACCTGGT CCTCACGGCC TCGGATGGCG 
     1681 GCAAGCCGCC TCGCTCTAGC ACAGTGCGCA TCCACGTGAC AGTGTTGGAT ACAAATGACA 
     1741 ATGCCCCGGT TTTTCCTCAC CCGATTTACC GAGTGAAAGT CCTTGAGAAC ATGCCCCCAG 
     1801 GCACGCGGCT GCTTACTGTA ACAGCCAGCG ACCCGGATGA GGGAATCAAC GGAAAAGTGG 
     1861 CATACAAATT CCGGAAAATT AATGAAAAAC AAACTCCGTT ATTCCAGCTT AATGAAAATA 
     1921 CTGGGGAAAT ATCAATAGCA AAAAGTCTAG ATTATGAAGA ATGTTCATTT TATGAAATGG 
     1981 AAATACAAGC CGAAGATGTG GGGGCACTTC TGGGGAGGAC CAAATTGCTC ATTTCGGTGG 
     2041 AAGATGTAAA TGACAATAGA CCAGAAGTGA TCATTACGTC TTTGTTTAGC CCAGTGTTAG 
     2101 AAAATTCTCT TCCCGGGACA GTAATTGCCT TCTTGAGTGT GCATGACCAA GACTCTGGAA 
     2161 AGAATGGTCA AGTTGTCTGT TACACACGTG ATAATTTACC TTTTAAATTA GAAAAGTCAA 
     2221 TAGGTAATTA TTATAGATTA GTGACAAGGA AATATTTGGA CCGAGAAAAT GTCTCTATCT 
     2281 ACAATATCAC AGTGATGGCC TCAGATCTAG GAACACCACC TCTGTCCACT GAAACTCAAA 
     2341 TCGCTCTGCA CGTGGCAGAC ATTAACGACA ACCCTCCTAC TTTCCCTCAT GCCTCCTACT 
     2401 CAGCGTATAT CCTAGAGAAC AACCTGAGAG GAGCCTCCAT CTTTTCCTTG ACTGCACACG 
     2461 ACCCCGACAG CCAGGAGAAT GCCCAGGTCA CTTACTCTGT GACCGAGGAC ACGCTGCAGG 
     2521 GGGCGCCCCT GTCCTCGTAT ATCTCCATCA ACTCTGACAC CGGTGTCCTG TATGCGCTGC 
     2581 AATCTTTCGA CTATGAGCAG ATCCGAGACC TGCAGCTACT GGTAACAGCC AGCGACAGCG 
     2641 GGGACCCGCC CCTCAGCAGC AACATGTCAC TGAGCCTGTT CGTGCTGGAC CAGAATGACA 
     2701 ACGCGCCCGA GATCCTGTAC CCCGCCCTCC CCACAGACGG TTCCACTGGC GTGGAGCTGG 
     2761 CGCCCCGCTC CGCAGAGCGT GGCTACCTGG TGACCAAGGT GGTGGCGGTG GACAGAGACT 
     2821 CGGGCCAGAA CGCCTGGCTG TCCTACCGCC TGCTCAAGGC CAGCGAGCCG GGACTCTTCT 
     2881 CGGTGGGTCT GCACACGGGC GAGGTGCGCA CGGCGCGAGC CCTGCTGGAC AGAGATGCGC 
     2941 TCAAGCAGAG CCTCGTGGTG GCCGTCCAGG ACCATGGCCA GCCCCCTCTC TCCGCCACTG 
     3001 TCACGCTCAC CGTAGCCGTG GCTGACAGCA TCCCCGAAGT CCTGACCGAG TTGGGCAGTC 
     3061 TGAAGCCTTC GGTCGACCCG AACGATTCGA GCCTTACACT CTATCTCGTG GTGGCAGTGG 
     3121 CTGCCATCTC CTGTGTCTTC CTCGCCTTTG TCGCTGTGCT TCTGGGGCTC AGGCTGAGGC 
     3181 GCTGGCACAA GTCACGCCTG CTCCAGGATT CCGGTGGCAG ATTGGTAGGC GTGCCTGCCT 
     3241 CACATTTTGT GGGTGTTGAG GAGGTACAGG CTTTCCTGCA GACCTATTCC CAGGAAGTCT 
     3301 CCCTCACCGC CGACTCGCGG AAGAGTCACC TGATCTTTCC CCAGCCCAAC TACGCAGACA 
     3361 TGCTCATCAG TCAGGAGGGC TGTGAGAAAA ATGATTCTTT GTTAACATCC GTAGATTTTC 
     3421 ATGAATATAA GAATGAAGCT GATCATGGTC AGCAAGCCCC GCCCAACACG GACTGGCGTT 
     3481 TCTCTCAGGC CCAGAGACCC GGCACCAGCG GCTCCCAAAA TGGCGATGAC ACCGGCACCT 
     3541 GGCCCAACAA CCAGTTTGAC ACAGAGATGC TGCAAGCCAT GATCTTGGCG TCCGCCAGTG 
     3601 AAGCTGCTGA TGGGAGCTCC ACCCTGGGAG GGGGTGCCGG CACCATGGGA TTGAGCGCCC 
     3661 GCTACGGACC CCAGTTCACC CTGCAGCACG TGCCCGACTA CCGCCAGAAT GTCTACATCC 
     3721 CAGGCAGCAA TGCCACACTG ACCAACGCAG CTGGCAAGCG GGATGGCAAG GCCCCAGCAG 
     3781 GTGGCAATGG CAACAAGAAG AAGTCGGGCA AGAAGGAGAA GAAGACGCGT ACGCGGCCGC 
     3841 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3901 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3961 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     4021 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     4081 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     4141 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     4201 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4261 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4321 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4381 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4441 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4501 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4561 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4621 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4681 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4741 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4801 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4861 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4921 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4981 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5041 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5101 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5161 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5221 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5281 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5341 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5401 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5461 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5521 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5581 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5641 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5701 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5761 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5821 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5881 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5941 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     6001 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     6061 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     6121 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     6181 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6241 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6301 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6361 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6421 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6481 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6541 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6601 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6661 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6721 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6781 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6841 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6901 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6961 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     7021 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     7081 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     7141 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     7201 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7261 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7321 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7381 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7441 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7501 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7561 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7621 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7681 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7741 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7801 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7861 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7921 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7981 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     8041 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     8101 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     8161 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     8221 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8281 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8341 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8401 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8461 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8521 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8581 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8641 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8701 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8761 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8821 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8881 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8941 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     9001 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     9061 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     9121 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     9181 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9241 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9301 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//