LOCUS       RG223929                9584 bp    DNA     circular  SYN 
DEFINITION  DHX36 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 36 (DHX36), transcript variant 1
ACCESSION   NM_020865
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4065..4769 
                     /label="GFP"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    7493..8138 
                     /label="ColE1"  
     misc_feature    5855..6649 
                     /label="Neo"  
     misc_feature    5486..5788 
                     /label="SV40 ori"  
     misc_feature    8153..9013 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4044..4050 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4044 
                     /label="DHX36"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTATGAC TACCATCAGA ACTGGGGCCG TGATGGGGGT CCCCGCAGCT 
     1081 CCGGTGGGGG CTATGGAGGG GGGCCAGCAG GGGGTCATGG AGGTAACCGA GGCTCCGGAG 
     1141 GAGGCGGCGG CGGCGGAGGG GGTGGTCGAG GCGGCAGGGG CCGGCATCCC GGGCACCTGA 
     1201 AAGGCCGCGA AATCGGCATG TGGTACGCGA AAAAACAGGG GCAGAAGAAC AAGGAAGCGG 
     1261 AGAGGCAAGA GAGAGCTGTA GTACACATGG ATGAACGACG AGAAGAACAA ATTGTACAGT 
     1321 TACTGAATTC TGTTCAAGCG AAGAATGATA AAGAGTCAGA AGCACAGATA TCCTGGTTTG 
     1381 CTCCTGAGGA TCATGGATAC GGTACTGAAG TTTCTACTAA GAACACACCA TGCTCAGAGA 
     1441 ACAAACTTGA CATCCAGGAA AAGAAGTTGA TAAATCAAGA AAAAAAAATG TTTAGAATCA 
     1501 GGAACAGATC ATATATTGAC CGAGATTCTG AGTATCTCTT GCAAGAAAAT GAACCAGATG 
     1561 GAACTTTAGA CCAAAAATTA TTGGAAGATT TACAAAAGAA AAAAAATGAC CTTCGGTATA 
     1621 TTGAAATGCA GCATTTCAGA GAAAAGCTGC CTTCGTATGG AATGCAAAAG GAATTGGTAA 
     1681 ATTTAATTGA TAACCATCAG GTAACAGTAA TAAGTGGTGA AACTGGTTGT GGCAAAACCA 
     1741 CTCAAGTTAC TCAGTTCATT TTGGATAACT ACATTGAAAG AGGAAAAGGA TCTGCTTGCA 
     1801 GAATAGTTTG TACTCAGCCA AGAAGAATTA GTGCCATTTC AGTTGCGGAA AGAGTAGCTG 
     1861 CAGAAAGGGC AGAATCTTGT GGCAGTGGTA ATAGTACTGG ATATCAAATT CGTCTCCAGA 
     1921 GTCGGTTGCC AAGGAAACAG GGTTCTATCT TATACTGTAC AACAGGAATC ATCCTTCAGT 
     1981 GGCTCCAGTC AGACCCGTAT TTGTCCAGTG TTAGTCATAT CGTACTTGAT GAAATCCATG 
     2041 AAAGAAATCT GCAGTCAGAT GTTTTAATGA CTGTTGTTAA AGACCTTCTC AATTTTCGAT 
     2101 CTGACTTGAA AGTAATATTG ATGAGTGCAA CATTGAATGC AGAAAAGTTT TCAGAATATT 
     2161 TTGGTAACTG TCCAATGATA CATATACCTG GTTTTACCTT TCCGGTTGTG GAATATCTTT 
     2221 TGGAAGATGT AATTGAAAAA ATAAGGTATG TTCCAGAACA AAAAGAACAC AGATGCCAGT 
     2281 TTAAGAGGGG TTTCATGCAA GGGCATGTAA ATAGACAAGA AAAAGAAGAA AAAGAAGCAA 
     2341 TATATAAAGA ACGTTGGCCA GATTATGTAA GGGAACTGCG AAGAAGGTAT TCTGCAAGTA 
     2401 CTGTAGATGT TATAGAAATG ATGGAGGATG ATAAAGTTGA TCTGAATTTG ATTGTTGCCC 
     2461 TCATCCGATA CATTGTTTTG GAAGAAGAGG ATGGTGCGAT ACTGGTCTTT CTGCCAGGCT 
     2521 GGGACAATAT CAGCACTTTA CATGATCTCT TGATGTCACA AGTAATGTTT AAATCAGATA 
     2581 AATTTTTAAT TATACCTTTA CATTCACTGA TGCCTACAGT TAACCAGACA CAGGTGTTTA 
     2641 AAAGAACCCC TCCTGGTGTT CGGAAAATAG TAATTGCTAC CAACATTGCG GAGACTAGCA 
     2701 TTACCATAGA TGATGTCGTT TATGTGATAG ATGGAGGAAA AATAAAAGAG ACGCATTTTG 
     2761 ATACTCAGAA CAATATCAGT ACAATGTCCG CTGAGTGGGT TAGTAAAGCT AATGCCAAAC 
     2821 AGAGAAAAGG TCGAGCTGGA AGAGTTCAAC CTGGTCATTG CTATCATCTG TATAATGGTC 
     2881 TTAGAGCAAG TCTTCTAGAT GACTATCAAC TGCCAGAAAT TTTGAGAACT CCTTTGGAAG 
     2941 AACTTTGTTT ACAAATAAAG ATTTTAAGGC TAGGTGGAAT TGCTTATTTT CTGAGTAGAT 
     3001 TAATGGACCC ACCATCAAAT GAGGCAGTGT TACTCTCCAT AAGACACCTG ATGGAGCTGA 
     3061 ACGCTTTGGA TAAACAAGAA GAATTGACAC CTCTTGGAGT CCACTTGGCA CGATTACCCG 
     3121 TTGAGCCACA TATTGGAAAA ATGATTCTTT TTGGAGCACT GTTCTGCTGC TTAGACCCAG 
     3181 TACTCACTAT TGCTGCTAGT CTCAGTTTCA AAGATCCATT TGTCATTCCA CTGGGAAAAG 
     3241 AAAAGATTGC AGATGCAAGA AGAAAGGAAT TGGCAAAGGA TACTAGAAGT GATCACTTAA 
     3301 CAGTTGTGAA TGCGTTTGAG GGCTGGGAAG AGGCTAGGCG ACGTGGTTTC AGATACGAAA 
     3361 AGGACTATTG CTGGGAATAT TTTCTGTCTT CAAACACACT GCAGATGCTG CATAACATGA 
     3421 AAGGACAGTT TGCTGAGCAT CTTCTTGGAG CTGGATTTGT AAGCAGTAGA AATCCTAAAG 
     3481 ATCCAGAATC TAATATAAAT TCAGATAATG AGAAGATAAT TAAAGCTGTC ATCTGTGCTG 
     3541 GTTTATATCC CAAAGTTGCT AAAATTCGAC TAAATTTGGG TAAAAAAAGA AAAATGGTAA 
     3601 AAGTTTACAC AAAAACCGAT GGCCTGGTTG CTGTTCATCC TAAATCTGTT AATGTGGAGC 
     3661 AAACAGACTT TCACTACAAC TGGCTTATCT ATCACCTAAA GATGAGAACA AGCAGTATAT 
     3721 ACTTGTATGA CTGCACAGAG GTTTCCCCAT ACTGTCTCTT GTTTTTTGGA GGTGACATTT 
     3781 CCATCCAGAA GGATAACGAT CAGGAAACTA TTGCTGTAGA TGAGTGGATT GTATTTCAGT 
     3841 CTCCAGCAAG AATTGCCCAT CTTGTTAAGG AATTAAGAAA GGAACTAGAT ATTCTTCTGC 
     3901 AAGAGAAGAT TGAAAGTCCT CATCCTGTAG ACTGGAATGA CACTAAATCC AGAGACTGTG 
     3961 CAGTACTGTC AGCTATTATA GACTTGATCA AAACACAGGA AAAGGCAACT CCCAGGAACT 
     4021 TTCCGCCACG ATTCCAGGAT GGATATTACA GCACGCGTAC GCGGCCGCTC GAGATGGAGA 
     4081 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     4141 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     4201 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4261 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4321 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4381 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4441 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4501 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4561 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4621 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4681 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4741 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4801 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4861 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4921 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4981 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5041 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5101 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5161 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5221 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5281 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5341 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5401 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5461 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5521 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5581 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5641 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5701 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5761 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5821 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5881 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5941 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     6001 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     6061 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     6121 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     6181 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6241 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6301 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6361 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6421 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6481 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6541 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6601 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6661 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6721 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6781 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6841 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6901 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6961 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     7021 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     7081 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     7141 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     7201 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7261 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7321 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7381 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7441 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7501 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7561 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7621 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7681 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7741 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7801 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7861 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7921 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7981 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     8041 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     8101 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     8161 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     8221 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8281 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8341 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8401 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8461 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8521 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8581 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8641 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8701 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8761 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8821 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8881 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8941 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     9001 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     9061 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     9121 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     9181 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9241 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9301 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9361 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9421 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9481 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9541 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//