LOCUS       RG223048                9167 bp    DNA     circular  SYN 
DEFINITION  GRIK2 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 2
ACCESSION   NM_175768
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3648..4352 
                     /label="GFP"  
     misc_feature    4399..4984 
                     /label="PolyA signal"  
     misc_feature    7076..7721 
                     /label="ColE1"  
     misc_feature    5438..6232 
                     /label="Neo"  
     misc_feature    5069..5371 
                     /label="SV40 ori"  
     misc_feature    7736..8596 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3627..3633 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3627 
                     /label="GRIK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGATTATT TTCCCGATTC TAAGTAATCC AGTCTTCAGG CGCACCGTTA 
     1081 AACTCCTGCT CTGTTTACTG TGGATTGGAT ATTCTCAAGG AACCACACAT GTATTAAGAT 
     1141 TTGGTGGTAT TTTTGAATAT GTGGAATCTG GCCCAATGGG AGCTGAGGAA CTTGCATTCA 
     1201 GATTTGCTGT GAACACAATT AACAGAAACA GAACATTGCT ACCCAATACT ACCCTTACCT 
     1261 ATGATACCCA GAAGATAAAC CTTTATGATA GTTTTGAAGC ATCCAAGAAA GCCTGTGATC 
     1321 AGCTGTCTCT TGGGGTGGCT GCCATCTTCG GGCCTTCACA CAGCTCATCA GCAAACGCAG 
     1381 TGCAGTCCAT CTGCAATGCT CTGGGAGTTC CCCACATACA GACCCGCTGG AAGCACCAGG 
     1441 TGTCAGACAA CAAAGATTCC TTCTATGTCA GTCTCTACCC AGACTTCTCT TCACTCAGCC 
     1501 GTGCCATTTT AGACCTGGTG CAGTTTTTCA AGTGGAAAAC CGTCACGGTT GTGTATGATG 
     1561 ACAGCACTGG TCTCATTCGT TTGCAAGAGC TCATCAAAGC TCCATCAAGG TATAATCTTC 
     1621 GACTCAAAAT TCGTCAGTTA CCTGCTGATA CAAAGGATGC AAAACCCTTA CTAAAAGAAA 
     1681 TGAAAAGAGG CAAGGAGTTT CATGTAATCT TTGATTGTAG CCATGAAATG GCAGCAGGCA 
     1741 TTTTAAAACA GGCATTAGCT ATGGGAATGA TGACAGAATA CTATCATTAT ATCTTTACCA 
     1801 CTCTGGACCT CTTTGCTCTT GATGTTGAGC CCTACCGATA CAGTGGTGTT AACATGACAG 
     1861 GGTTCAGAAT ATTAAATACA GAAAATACCC AAGTCTCCTC CATCATTGAA AAGTGGTCGA 
     1921 TGGAACGATT GCAGGCACCT CCGAAACCCG ATTCAGGTTT GCTGGATGGA TTTATGACGA 
     1981 CTGATGCTGC TCTAATGTAT GATGCTGTGC ATGTGGTGTC TGTGGCCGTT CAACAGTTTC 
     2041 CCCAGATGAC AGTCAGTTCC TTGCAGTGTA ATCGACATAA ACCCTGGCGC TTCGGGACCC 
     2101 GCTTTATGAG TCTAATTAAA GAGGCACATT GGGAAGGCCT CACAGGCAGA ATAACTTTCA 
     2161 ACAAAACCAA TGGCTTGAGA ACAGATTTTG ATTTGGATGT GATCAGTCTG AAGGAAGAAG 
     2221 GTCTAGAAAA GATTGGAACG TGGGATCCAG CCAGTGGCCT GAATATGACA GAAAGTCAAA 
     2281 AGGGAAAGCC AGCGAACATC ACAGATTCCT TATCCAATCG TTCTTTGATT GTTACCACCA 
     2341 TTTTGGAAGA GCCTTATGTC CTTTTTAAGA AGTCTGACAA ACCTCTCTAT GGTAATGATC 
     2401 GATTTGAAGG CTATTGCATT GATCTCCTCA GAGAGTTATC TACAATCCTT GGCTTTACAT 
     2461 ATGAAATTAG ACTTGTGGAA GATGGGAAAT ATGGAGCCCA GGATGATGCC AATGGACAAT 
     2521 GGAATGGAAT GGTTCGTGAA CTAATTGATC ATAAAGCTGA CCTTGCAGTT GCTCCACTGG 
     2581 CTATTACCTA TGTTCGAGAG AAGGTCATCG ACTTTTCCAA GCCCTTTATG ACACTTGGAA 
     2641 TAAGTATTTT GTACCGCAAG CCCAATGGTA CAAACCCAGG CGTCTTCTCC TTCCTGAATC 
     2701 CTCTCTCCCC TGATATCTGG ATGTATATTC TGCTGGCTTA CTTGGGTGTC AGTTGTGTGC 
     2761 TCTTTGTCAT AGCCAGGTTT AGTCCTTATG AGTGGTATAA TCCACACCCT TGCAACCCTG 
     2821 ACTCAGACGT GGTGGAAAAC AATTTTACCT TGCTAAATAG TTTCTGGTTT GGAGTTGGAG 
     2881 CTCTCATGCA GCAAGGTTCT GAGCTCATGC CCAAAGCACT GTCCACCAGG ATAGTGGGAG 
     2941 GCATTTGGTG GTTTTTCACA CTTATCATCA TTTCTTCGTA TACTGCTAAC TTAGCCGCCT 
     3001 TTCTGACAGT GGAACGCATG GAATCCCCTA TTGACTCTGC TGATGATTTA GCTAAACAAA 
     3061 CCAAGATAGA ATATGGAGCA GTAGAGGATG GTGCAACCAT GACTTTTTTC AAGAAATCAA 
     3121 AAATCTCCAC GTATGACAAA ATGTGGGCCT TTATGAGTAG CAGAAGGCAG TCAGTGCTGG 
     3181 TCAAAAGTAA TGAAGAAGGA ATCCAGCGAG TCCTCACCTC TGATTATGCT TTCCTAATGG 
     3241 AGTCAACAAC CATCGAGTTT GTTACCCAGC GGAACTGTAA CCTGACACAG ATTGGCGGCC 
     3301 TTATAGACTC TAAAGGTTAT GGCGTTGGCA CTCCCATGGG TTCTCCATAT CGAGACAAAA 
     3361 TTACCATAGC AATTCTTCAG CTGCAAGAGG AAGGCAAACT GCATATGATG AAGGAGAAAT 
     3421 GGTGGAGGGG CAATGGTTGC CCAGAAGAGG AGAGCAAAGA GGCCAGTGCC CTGGGGGTTC 
     3481 AGAATATTGG TGGCATCTTC ATTGTTCTGG CAGCCGGCTT GGTGCTTTCA GTTTTTGTGG 
     3541 CAGTGGGAGA ATTTTTATAC AAATCCAAAA AAAACGCTCA ATTGGAAAAG GAATCTTCTA 
     3601 TTTGGTTAGT GCCACCATAC CATCCAGACA CTGTTACGCG TACGCGGCCG CTCGAGATGG 
     3661 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3721 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3781 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3841 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3901 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     3961 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     4021 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4081 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4141 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4201 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4261 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4321 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4381 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4441 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4501 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4561 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4621 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4681 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4741 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4801 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4861 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4921 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4981 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     5041 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5101 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5161 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5221 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5281 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5341 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5401 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5461 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5521 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5581 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5641 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5701 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5761 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5821 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5881 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     5941 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     6001 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6061 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6121 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6181 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6241 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6301 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6361 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6421 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6481 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6541 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6601 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6661 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6721 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6781 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6841 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6901 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     6961 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     7021 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7081 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7141 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7201 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7261 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7321 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7381 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7441 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7501 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7561 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7621 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7681 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7741 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7801 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7861 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7921 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     7981 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     8041 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8101 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8161 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8221 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8281 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8341 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8401 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8461 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8521 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8581 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8641 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8701 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8761 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8821 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8881 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     8941 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     9001 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9061 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9121 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//