LOCUS       RG223042                8987 bp    DNA     circular  SYN 
DEFINITION  PGBD1 (tGFP-tagged) - Human piggyBac transposable element derived 1 (PGBD1), transcript variant 2
ACCESSION   NM_032507
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3468..4172 
                     /label="GFP"  
     misc_feature    4219..4804 
                     /label="PolyA signal"  
     misc_feature    6896..7541 
                     /label="ColE1"  
     misc_feature    5258..6052 
                     /label="Neo"  
     misc_feature    4889..5191 
                     /label="SV40 ori"  
     misc_feature    7556..8416 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3447..3453 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3447 
                     /label="PGBD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGAAGCT TTGCCAGGCC CTGCTCCTGA AAATGAAGAT GGCCTTGTGA 
     1081 AAGTGAAGGA GGAAGATCCC ACCTGGGAGC AGGTGTGCAA CTCACAGGAG GGCAGCTCCC 
     1141 ACACTCAGGA GATTTGCCGC CTGCGCTTTC GGCACTTCTG CTACCAGGAG GCTCACGGAC 
     1201 CCCAGGAAGC TCTGGCCCAA CTCCGAGAAC TTTGTCATCA ATGGCTGAGA CCGGAGATGC 
     1261 ACACCAAGGA ACAGATAATG GAACTGCTGG TGCTGGAGCA GTTCCTGACC ATCCTGCCCA 
     1321 AGGAGCTCCA GCCCTGTGTG AAGACATATC CTCTGGAGAG TGGAGAGGAG GCAGTGACAG 
     1381 TGCTGGAGAA TCTAGAGACA GGAAGTGGAG ACACAGGACA ACAGGCCTCT GTCTATATTC 
     1441 AGGGACAGGA CATGCACCCA ATGGTGGCAG AATATCAAGG AGTCTCTTTG GAGTGTCAGA 
     1501 GCCTCCAGCT CCTGCCTGGG ATAACCACCC TGAAGTGTGA ACCTCCACAG CGTCCTCAAG 
     1561 GGAACCCCCA AGAAGTGAGT GGGCCTGTTC CCCACGGATC AGCTCATCTC CAGGAAAAAA 
     1621 ACCCCAGAGA CAAGGCTGTA GTGCCTGTGT TTAACCCAGT CAGGTCCCAG ACATTGGTGA 
     1681 AGACTGAGGA AGAAACAGCC CAGGCCGTTG CTGCAGAGAA GTGGTCACAT CTGAGTCTGA 
     1741 CTCGGAGGAA CCTCTGTGGG AACTCAGCTC AGGAGACAGT TATGAGCCTC AGTCCGATGA 
     1801 CTGAAGAAAT TGTAACTAAA GATAGATTGT TTAAAGCAAA GCAAGAAACT TCTGAAGAAA 
     1861 TGGAACAAAG TGGAGAAGCC TCAGGAAAGC CCAACAGAGA GTGTGCACCC CAGATTCCTT 
     1921 GTAGTACTCC TATTGCTACT GAAAGGACAG TTGCACATTT GAACACTCTG AAGGACCGTC 
     1981 ACCCAGGTGA TTTGTGGGCC CGCATGCACA TCTCATCCCT GGAATATGCT GCAGGAGACA 
     2041 TTACCCGAAA AGGGAGAAAA AAAGACAAAG CTCGAGTGAG TGAACTGCTC CAAGGCCTCT 
     2101 CATTCTCTGG TGACTCAGAT GTGGAAAAAG ATAATGAGCC TGAGATCCAG CCTGCTCAAA 
     2161 AGAAGTTAAA GGTATCATGT TTCCCAGAAA AGAGTTGGAC CAAAAGAGAC ATTAAACCCA 
     2221 ATTTTCCAAG CTGGTCAGCA CTGGATTCTG GACTTTTGAA TCTCAAGAGC GAAAAGTTGA 
     2281 ACCCAGTAGA GCTTTTTGAA TTATTTTTTG ATGATGAAAC ATTCAACTTA ATTGTCAATG 
     2341 AAACCAATAA TTATGCTTCT CAGAAAAATG TCAGCTTGGA AGTCACAGTT CAGGAAATGA 
     2401 GGTGTGTGTT TGGTGTCTTA CTTTTGAGTG GATTTATGAG GCATCCTAGA AGGGAAATGT 
     2461 ATTGGGAAGT CTCTGACACC GATCAGAACC TGGTTAGAGA TGCAATCAGA AGGGACAGAT 
     2521 TTGAATTGAT TTTCTCAAAC CTGCACTTTG CAGATAATGG CCACCTAGAT CAAAAAGATA 
     2581 AGTTTACAAA GTTGAGACCT CTCATAAAAC AAATGAATAA AAATTTCCTC TTGTATGCTC 
     2641 CCCTGGAAGA ATACTATTGC TTTGATAAGT CAATGTGTGA ATGCTTTGAT AGTGACCAAT 
     2701 TCCTGAATGG AAAGCCTATT AGAATTGGCT ATAAAATTTG GTGTGGTACA ACCACACAGG 
     2761 GTTATCTGGT TTGGTTTGAA CCCTATCAAG AAGAATCAAC TATGAAGGTA GATGAGGATC 
     2821 CTGATCTTGG GTTAGGTGGA AATCTAGTGA TGAACTTCGC TGATGTTCTT TTAGAGAGAG 
     2881 GTCAGTATCC CTATCACCTG TGTTTTGATA GCTTCTTTAC AAGTGTCAAA TTGTTGTCAG 
     2941 CCTTGAAAAA GAAGGGGGTG AGGGCAACAG GAACAATTCG TGAGAACAGG ACCGAAAAAT 
     3001 GTCCCCTTAT GAATGTAGAA CATATGAAAA AAATGAAGAG AGGGTATTTT GATTTCCGAA 
     3061 TAGAAGAAAA CAATGAGATA ATTTTGTGTC GTTGGTATGG GGATGGCATT ATCAGTCTGT 
     3121 GCTCCAATGC TGTGGGCATA GAACCAGTCA ATGAGGTAAG CTGTTGTGAT GCTGATAACG 
     3181 AAGAAATCCC TCAGATAAGT CAACCATCCA TAGTAAAAGT GTATGATGAA TGCAAGGAAG 
     3241 GTGTAGCTAA AATGGATCAA ATTATTTCGA AATACAGGGT GAGGATAAGA AGCAAGAAAT 
     3301 GGTACTCAAT TTTGGTGAGC TACATGATTG ATGTAGCCAT GAACAATGCA TGGCAACTAC 
     3361 ACAGAGCCTG TAACCCAGGT GCTTCTCTAG ACCCCTTGGA TTTTCGGAGA TTTGTTGCAC 
     3421 ATTTCTACTT GGAACACAAT GCTCATCTGT CAGATACGCG TACGCGGCCG CTCGAGATGG 
     3481 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3541 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3601 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3661 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3721 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     3781 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     3841 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     3901 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     3961 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4021 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4081 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4141 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4201 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4261 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4321 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4381 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4441 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4501 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4561 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4621 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4681 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4741 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4801 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     4861 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     4921 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     4981 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5041 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5101 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5161 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5221 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5281 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5341 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5401 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5461 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5521 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5581 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5641 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5701 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     5761 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     5821 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     5881 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     5941 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6001 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6061 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6121 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6181 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6241 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6301 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6361 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6421 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6481 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6541 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6601 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6661 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6721 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     6781 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     6841 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     6901 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     6961 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7021 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7081 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7141 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7201 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7261 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7321 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7381 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7441 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7501 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7561 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7621 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7681 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7741 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     7801 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     7861 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     7921 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     7981 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8041 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8101 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8161 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8221 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8281 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8341 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8401 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8461 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8521 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8581 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8641 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8701 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     8761 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     8821 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     8881 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     8941 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//