LOCUS       RG222491                9287 bp    DNA     circular  SYN 
DEFINITION  TMC2 (tGFP-tagged) - Human transmembrane channel-like 2 (TMC2)
ACCESSION   NM_080751
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3766..4470 
                     /label="GFP"  
     misc_feature    4517..5102 
                     /label="PolyA signal"  
     misc_feature    7194..7839 
                     /label="ColE1"  
     misc_feature    5556..6350 
                     /label="Neo"  
     misc_feature    5187..5489 
                     /label="SV40 ori"  
     misc_feature    7854..8714 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3738..3745 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3738 
                     /label="TMC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCACCAG GTAAAGGGCC TGAAAGAGGA AGCACGAGGC GGAGTGAAAG 
     1081 GGCGGGTGAA GAGCGGCTCT CCACACACAG GTGACAGGCT GGGAAGGAGA TCCTCAAGCA 
     1141 AGCGGGCTCT CAAAGCCGAG GGGACCCCAG GCAGGCGCGG AGCTCAGCGA AGCCAGAAGG 
     1201 AGCGCGCCGG GGGCAGCCCA AGCCCGGGGT CTCCCCGGAG GAAGCAAACA GGGCGCAGGA 
     1261 GACACAGAGA AGAGCTGGGG GAGCAGGAGC GGGGCGAGGC AGAGAGGACC TGCGAGGGCA 
     1321 GGAGAAAGCG CGACGAGAGG GCCTCCTTCC AGGAGCGGAC AGCAGCCCCA AAGAGGGAAA 
     1381 AGGAGATTCC GAGGAGGGAG GAGAAGTCGA AGCGGCAGAA GAAACCCAGG TCATCCTCCT 
     1441 TGGCCTCCAG TGCCTCTGGT GGGGAGTCCC TGTCCGAGGA GGAACTGGCC CAGATCCTGG 
     1501 AGCAGGTGGA AGAAAAAAAG AAGCTCATTG CCACCATGCG GAGCAAGCCC TGGCCCATGG 
     1561 CGAAGAAGCT GACAGAGCTC AGGGAGGCCC AGGAATTTGT GGAGAAGTAT GAAGGTGCCT 
     1621 TGGGAAAGGG GAAAGGCAAG CAACTATATG CCTACAAGAT GCTGATGGCC AAGAAATGGG 
     1681 TCAAATTTAA GAGAGACTTT GATAATTTCA AGACTCAATG TATCCCCTGG GAAATGAAGA 
     1741 TCAAGGACAT TGAAAGTCAC TTTGGTTCTT CAGTGGCATC GTATTTCATC TTTCTCCGAT 
     1801 GGATGTATGG AGTTAACCTT GTCCTTTTTG GCTTAATATT TGGTCTAGTC ATAATCCCAG 
     1861 AGGTACTGAT GGGCATGCCC TATGGGAGTA TTCCCAGAAA GACAGTGCCT CGGGCTGAGG 
     1921 AAGAAAAGGC CATGGATTTT TCTGTCCTTT GGGATTTTGA GGGCTATATC AAGTACTCTG 
     1981 CACTCTTCTA TGGCTACTAC AACAACCAGA GGACCATCGG GTGGCTGAGG TACCGGCTGC 
     2041 CTATGGCTTA CTTTATGGTG GGGGTCAGCG TGTTCGGCTA CAGCCTGATT ATTGTCATTC 
     2101 GATCGATGGC CAGCAATACC CAAGGAAGCA CAGGCGAAGG GGAGAGTGAC AACTTCACAT 
     2161 TCAGCTTCAA GATGTTCACC AGCTGGGACT ACCTGATCGG GAATTCAGAG ACAGCTGATA 
     2221 ACAAATATGC ATCCATCACC ACCAGCTTCA AGGAATCAAT AGTGGATGAA CAAGAGAGTA 
     2281 ACAAAGAAGA AAATATCCAT CTGACAAGAT TTCTTCGTGT CCTGGCCAAC TTTCTCATCA 
     2341 TCTGCTGTTT GTGTGGAAGT GGGTACCTCA TTTACTTTGT GGTTAAGCGA TCTCAGCAAT 
     2401 TCTCCAAAAT GCAGAATGTC AGCTGGTATG AAAGGAATGA GGTAGAGATC GTGATGTCCC 
     2461 TGCTTGGAAT GTTTTGTCCC CCTCTGTTTG AAACCATCGC TGCCCTGGAG AATTACCACC 
     2521 CACGCACTGG ACTGAAGTGG CAGCTGGGAC GCATCTTTGC ACTCTTCCTG GGGAACCTCT 
     2581 ACACATTTCT CTTGGCCCTG ATGGATGACG TCCACCTCAA GCTTGCTAAT GAAGAGACAA 
     2641 TAAAGAACAT CACTCACTGG ACTCTGTTTA ACTATTACAA CTCTTCTGGT TGGAACGAGA 
     2701 GTGTCCCCCG ACCACCCCTG CACCCTGCAG ATGTGCCCCG GGGTTCTTGC TGGGAGACAG 
     2761 CTGTGGGCAT TGAATTCATG AGGCTGACGG TGTCTGACAT GCTGGTAACG TACATCACCA 
     2821 TCCTGCTGGG GGACTTCCTA CGGGCTTGTT TTGTGCGGTT CATGAACTAC TGCTGGTGCT 
     2881 GGGACTTGGA GGCTGGATTT CCTTCATATG CTGAGTTTGA TATTAGTGGA AATGTGCTGG 
     2941 GTTTGATCTT CAACCAAGGA ATGATCTGGA TGGGCTCCTT CTATGCTCCA GGCCTGGTGG 
     3001 GCATTAATGT GCTGCGCCTG CTGACCTCCA TGTACTTCCA GTGCTGGGCG GTGATGAGCA 
     3061 GCAACGTACC CCATGAACGC GTGTTCAAAG CCTCCCGATC CAACAACTTC TACATGGGCC 
     3121 TCCTGCTGCT GGTGCTCTTC CTCAGCCTCC TGCCGGTGGC CTACACCATC ATGTCCCTCC 
     3181 CACCCTCCTT TGACTGCGGG CCGTTCAGTG GGAAAAACAG AATGTACGAT GTCCTCCAAG 
     3241 AGACCATTGA AAACGATTTC CCAACCTTCC TGGGCAAGAT CTTTGCTTTC CTCGCCAATC 
     3301 CAGGCCTGAT CATCCCAGCC ATCCTGCTGA TGTTCTTGGC CATTTACTAC CTGAACTCAG 
     3361 TTTCCAAAAG CCTTTCCCGA GCTAATGCCC AGCTGAGGAA GAAAATCCAA GTGCTCCGTG 
     3421 AAGTTGAGAA GAGTCACAAA TCTGTAAAAG GCAAAGCCAC AGCCAGAGAT TCAGAGGACA 
     3481 CACCTAAAAG CAGCTCCAAA AATGCCACCC AGCTCCAACT CACCAAGGAA GAGACCACTC 
     3541 CTCCCTCTGC CAGCCAAAGC CAGGCCATGG ACAAGAAGGC GCAGGGCCCT GGGACCTCCA 
     3601 ATTCTGCCAG CAGGACCACA CTGCCTGCCT CTGGACACCT TCCTATATCT CGGCCCCCTG 
     3661 GAATCGGACC AGATTCTGGC CACGCCCCAT CTCAGACTCA TCCGTGGAGG TCAGCCTCTG 
     3721 GAAAGAGTGC TCAGAGACCT CCCCACAGCG GACCGACGCG TACGCGGCCG CTCGAGATGG 
     3781 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3841 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3901 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3961 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     4021 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     4081 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     4141 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4201 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4261 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4321 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4381 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4441 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4501 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4561 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4621 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4681 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4741 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4801 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4861 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4921 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4981 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5041 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5101 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     5161 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5221 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5281 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5341 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5401 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5461 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5521 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5581 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5641 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5701 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5761 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5821 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5881 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5941 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     6001 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     6061 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     6121 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6181 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6241 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6301 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6361 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6421 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6481 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6541 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6601 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6661 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6721 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6781 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6841 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6901 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6961 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     7021 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     7081 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     7141 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7201 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7261 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7321 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7381 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7441 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7501 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7561 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7621 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7681 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7741 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7801 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7861 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7921 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7981 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     8041 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     8101 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     8161 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8221 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8281 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8341 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8401 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8461 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8521 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8581 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8641 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8701 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8761 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8821 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8881 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8941 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     9001 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     9061 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     9121 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9181 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9241 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//