LOCUS       RG222369                9047 bp    DNA     circular  SYN 
DEFINITION  GRIK2 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1
ACCESSION   NM_021956
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3528..4232 
                     /label="GFP"  
     misc_feature    4279..4864 
                     /label="PolyA signal"  
     misc_feature    6956..7601 
                     /label="ColE1"  
     misc_feature    5318..6112 
                     /label="Neo"  
     misc_feature    4949..5251 
                     /label="SV40 ori"  
     misc_feature    7616..8476 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3507..3513 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3507 
                     /label="GRIK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATAGTTTTG AAGCATCCAA GAAAGACCTT TATCTCCTCT TCAGCCTGTG 
     1081 ATCAGCTGTC TCTTGGGGTG GCTGCCATCT TCGGGCCTTC ACACAGCTCA TCAGCAAACG 
     1141 CAGTGCAGTC CATCTGCAAT GCTCTGGGAG TTCCCCACAT ACAGACCCGC TGGAAGCACC 
     1201 AGGTGTCAGA CAACAAAGAT TCCTTCTATG TCAGTCTCTA CCCAGACTTC TCTTCACTCA 
     1261 GCCGTGCCAT TTTAGACCTG GTGCAGTTTT TCAAGTGGAA AACCGTCACG GTTGTGTATG 
     1321 ATGACAGCAC TGGTCTCATT CGTTTGCAAG AGCTCATCAA AGCTCCATCA AGGTATAATC 
     1381 TTCGACTCAA AATTCGTCAG TTACCTGCTG ATACAAAGGA TGCAAAACCC TTACTAAAAG 
     1441 AAATGAAAAG AGGCAAGGAG TTTCATGTAA TCTTTGATTG TAGCCATGAA ATGGCAGCAG 
     1501 GCATTTTAAA ACAGGCATTA GCTATGGGAA TGATGACAGA ATACTATCAT TATATCTTTA 
     1561 CCACTCTGGA CCTCTTTGCT CTTGATGTTG AGCCCTACCG ATACAGTGGT GTTAACATGA 
     1621 CAGGGTTCAG AATATTAAAT ACAGAAAATA CCCAAGTCTC CTCCATCATT GAAAAGTGGT 
     1681 CGATGGAACG ATTGCAGGCA CCTCCGAAAC CCGATTCAGG TTTGCTGGAT GGATTTATGA 
     1741 CGACTGATGC TGCTCTAATG TATGATGCTG TGCATGTGGT GTCTGTGGCC GTTCAACAGT 
     1801 TTCCCCAGAT GACAGTCAGT TCCTTGCAGT GTAATCGACA TAAACCCTGG CGCTTCGGGA 
     1861 CCCGCTTTAT GAGTCTAATT AAAGAGGCAC ATTGGGAAGG CCTCACAGGC AGAATAACTT 
     1921 TCAACAAAAC CAATGGCTTG AGAACAGATT TTGATTTGGA TGTGATCAGT CTGAAGGAAG 
     1981 AAGGTCTAGA AAAGATTGGA ACGTGGGATC CAGCCAGTGG CCTGAATATG ACAGAAAGTC 
     2041 AAAAGGGAAA GCCAGCGAAC ATCACAGATT CGTTATCCAA TCGTTCTTTG ATTGTTACCA 
     2101 CCATTTTGGA AGAGCCTTAT GTCCTTTTTA AGAAGTCTGA CAAACCTCTC TATGGTAATG 
     2161 ATCGATTTGA AGGCTATTGC ATTGATCTCC TCAGAGAGTT ATCTACAATC CTTGGCTTTA 
     2221 CATATGAAAT TAGACTTGTG GAAGATGGGA AATATGGAGC CCAGGATGAT GCCAATGGAC 
     2281 AATGGAATGG AATGGTTCGT GAACTAATTG ATCATAAAGC TGACCTTGCA GTTGCTCCAC 
     2341 TGGCTATTAC CTATGTTCGA GAGAAGGTCA TCGACTTTTC CAAGCCCTTT ATGACACTTG 
     2401 GAATAAGTAT TTTGTACCGC AAGCCCAATG GTACAAACCC AGGCGTCTTC TCCTTCCTGA 
     2461 ATCCTCTCTC CCCTGATATC TGGATGTATA TTCTGCTGGC TTGCTTGGGT GTCAGTTGTG 
     2521 TGCTCTTTGT CATAGCCAGG TTTAGTCCTT ATGAGTGGTA TAATCCACAC CCTTGCAACC 
     2581 CTGACTCAGA CGTGGTGGAA AACAATTTTA CCTTGCTAAA TAGTTTCTGG TTTGGAGTTG 
     2641 GAGCTCTCAT GCGGCAAGGT TCTGAGCTCA TGCCCAAAGC ACTGTCCACC AGGATAGTGG 
     2701 GAGGCATTTG GTGGTTTTTC ACACTTATCA TCATTTCTTC GTATACTGCT AACTTAGCCG 
     2761 CCTTTCTGAC AGTGGAACGC ATGGAATCCC CTATTGACTC TGCTGATGAT TTAGCTAAAC 
     2821 AAACCAAGAT AGAATATGGA GCAGTAGAGG ATGGTGCAAC CATGACTTTT TTCAAGAAAT 
     2881 CAAAAATCTC CACGTATGAC AAAATGTGGG CCTTTATGAG TAGCAGAAGG CAGTCAGTGC 
     2941 TGGTCAAAAG TAATGAAGAA GGAATCCAGC GAGTCCTCAC CTCTGATTAT GCTTTCCTAA 
     3001 TGGAGTCAAC AACCATCGAG TTTGTTACCC AGCGGAACTG TAACCTGACA CAGATTGGCG 
     3061 GCCTTATAGA CTCTAAAGGT TATGGCGTTG GCACTCCCAT GGGTTCTCCA TATCGAGACA 
     3121 AAATTACCAT AGCAATTCTT CAGCTGCAAG AGGAAGGCAA ACTGCATATG ATGAAGGAGA 
     3181 AATGGTGGAG GGGCAATGGT TGCCCAGAAG AGGAGAGCAA AGAGGCCAGT GCCCTGGGGG 
     3241 TTCAGAATAT TGGTGGCATC TTCATTGTTC TGGCAGCCGG CTTGGTGCTT TCAGTTTTTG 
     3301 TGGCAGTGGG AGAATTTTTA TACAAATCCA AAAAAAACGC TCAATTGGAA AAGAGGTCCT 
     3361 TCTGTAGTGC CATGGTAGAA GAATTGAGGA TGTCCCTGAA GTGCCAGCGT CGGTTAAAAC 
     3421 ATAAGCCACA GGCCCCAGTT ATTGTGAAAA CAGAAGAAGT TATCAACATG CACACATTTA 
     3481 ACGACAGAAG GTTGCCAGGT AAAGAAACCA TGGCAACGCG TACGCGGCCG CTCGAGATGG 
     3541 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3601 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3661 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3721 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3781 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     3841 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     3901 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     3961 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4021 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4081 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4141 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4201 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4261 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4321 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4381 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4441 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4501 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4561 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4621 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4681 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4741 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4801 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4861 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     4921 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     4981 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5041 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5101 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5161 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5221 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5281 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5341 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5401 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5461 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5521 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5581 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5641 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5701 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5761 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     5821 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     5881 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     5941 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6001 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6061 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6121 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6181 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6241 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6301 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6361 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6421 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6481 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6541 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6601 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6661 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6721 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6781 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     6841 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     6901 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     6961 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7021 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7081 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7141 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7201 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7261 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7321 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7381 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7441 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7501 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7561 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7621 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7681 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7741 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7801 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     7861 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     7921 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     7981 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8041 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8101 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8161 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8221 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8281 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8341 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8401 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8461 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8521 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8581 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8641 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8701 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8761 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     8821 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     8881 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     8941 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9001 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//