LOCUS       RG222084                8984 bp    DNA     circular  SYN 
DEFINITION  PCDHGB7 (tGFP-tagged) - Human protocadherin gamma subfamily B, 7 (PCDHGB7), transcript variant 2
ACCESSION   NM_032101
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3465..4169 
                     /label="GFP"  
     misc_feature    4216..4801 
                     /label="PolyA signal"  
     misc_feature    6893..7538 
                     /label="ColE1"  
     misc_feature    5255..6049 
                     /label="Neo"  
     misc_feature    4886..5188 
                     /label="SV40 ori"  
     misc_feature    7553..8413 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3444..3450 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3444 
                     /label="PCDHGB7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGGGAGC TGCGCGCAGA GGCGCCGGGC CGGCCCGCGG CAGGTACTAT 
     1081 TTCCTTTGCT GCTGCCTTTG TTCTACCCCA CGCTGTGTGA GCCGATCCGC TACTCGATTC 
     1141 CGGAGGAGCT GGCCAAGGGC TCGGTGGTGG GGAACCTCGC TAAGGATCTA GGGCTTAGTG 
     1201 TCCTGGATGT GTCGGCTCGC GAGCTGCGAG TGAGCGCGGA GAAGCTGCAC TTCAGCGTAG 
     1261 ACGCGCAGAG CGGGGACTTA CTTGTGAAGG ACCGAATAGA CCGTGAGCAA ATATGCAAAG 
     1321 AGAGAAGAAG ATGTGAGTTG CAATTGGAAG CTGTGGTGGA AAATCCTTTA AATATTTTTC 
     1381 ATGTCATTGT GGTGATTGAG GATGTTAATG ACCACGCCCC TCAATTCCGG AAAGATGAAA 
     1441 TAAACTTAGA AATCAGTGAA TCCGTCAGCC TGGGGATGGG AACAATTCTT GAGTCTGCAG 
     1501 AAGATCCTGA TATTAGTATG AATTCGCTGA GCAAATACCA ACTAAGTCCT AACGAGTATT 
     1561 TCTCATTGGT GGAGAAAGAC AATCCTGATG GTGGCAAATA TCCAGAATTA GTATTGCAGA 
     1621 AGACTCTGGA CCGAGAAACG CAGAGCGCTC ACCACTTGGT ACTGACCGCC TTAGATGGTG 
     1681 GGGACCCTCC CCGAAGCGGT ACTGCTCAGA TAAGAATCCT GGTAATAGAT GCCAATGACA 
     1741 ACCCCCCAGT GTTCAGCCAG GACGTGTACA GGGTTAGCCT TCGGGAAGAC GTGCCTCCAG 
     1801 GCACCTCCAT CCTGAGAGTG AAGGCCACTG ACCAGGACGA GGGCATCAAC TCAGAGATCA 
     1861 CTTATTCCTT CTTTGGTGTG GCTGACAAAG CTCAGCACGT GTTCTCTCTG GATTACACTA 
     1921 CAGGAAACAT TCTAACTCAG CAGCCTTTGG ATTTTGAAGA AGTAGAAAGA TATACGATAA 
     1981 ACATAGAAGC AAAAGACCGA GGATCTCTCT CAACACGGTG TAAAGTAATT GTAGAAGTTG 
     2041 TAGACGAAAA CGACAACAGC CCAGAAATAA TCATCACGTC ACTCTCTGAT CAGATTATGG 
     2101 AGGATTCCCC TCCAGGAGTG GTTGTTGCCC TCTTCAAAAC ACGGGACCAA GACTCAGGGG 
     2161 AAAATGGGGA AGTCAGGTGT AGCTTAAGTA GAGGTGTTCC ATTTAAGATT CATTCTTCTT 
     2221 CTAATAATTA CTACAAGCTA GTAACAGATG AGGCCCTGGA TCGGGAGCAG ACCCCAGAGT 
     2281 ACAACGTCAC CATCGCAGCC ACAGACAGGG GCAAGCCTCC GTTATCCTCC AGCAAAACCA 
     2341 TAACCCTGCA CATTACTGAC GTCAATGACA ACGCGCCGGT TTTCGGACAG TCAGCCTACC 
     2401 TGGTCCACGT GCCAGAAAAC AACCAGCCGG GTGCCTCCAT AGCGCAAGTC AGTGCCTCTG 
     2461 ACCCAGACTT CGGGCTCAAC GGCCGTGTCT CCTACTCTCT CATTGCCAGC GACCTGGAGT 
     2521 CACGAACGCT GTCGTCCTAC GTGTCCGTGA GCGCGCAGAG CGGGGTGGTG TTCGCGCAGC 
     2581 GCGCCTTCGA CCACGAGCAG CTGCGCACCT TCGAGCTCAC GCTGCAGGCC CGCGACCAGG 
     2641 GCTCGCCCGC GCTCAGCGCC AATGTGAGCC TGCGCGTGTT GGTGGGCGAC CGTAACGACA 
     2701 ACGCACCGCG GGTGCTGTAC CCTGCGCTGG GTCCCGACGG CTCCGCGCTC TTCGACACAG 
     2761 TGCCGCGGGC CGCGCAGCCA GGCTACCTGG TGACCAAGGT GGTGGCCGTG GACGCGGACT 
     2821 CGGGGCACAA TGCCTGGCTG TCCTACCACG TGGTGCAGGC CAGTGAGCCC GGGCTCTTCA 
     2881 GCCTGGGGCT GCGAACAGGC GAGGTGCGCA TGGTGCGTGC TTTGGGTGAC AAGGACTCGG 
     2941 TCCGCCAGCG CCTGCTAGTC GCTGTAAGAG ATGGAGGACA GCCACCCCTT TCAGCCACTG 
     3001 CCACGCTGCA CCTGGTGTTC GCAGATAGCT TGCAAGAGGT ACTGCCGGAT TTCAGCGACC 
     3061 ATCCCACACC CTCTGACTCC CAGGCTGAGA TGCAGTTTTA CCTGGTGGTG GCCTTGGCCT 
     3121 TGATTTCTGT GCTCTTTCTC CTCGCGGTGA TTCTAGCTAT TGCTCTACGC CTGCGACAGT 
     3181 CTTTCAGCCC TACTGCAGGA GACTGCTTTG AGTCAGTTCT CTGCTCCAAG TCCGGACCTG 
     3241 TGGGTCCCCC CAACTACAGT GAGGGAACGT TGCCCTATGC CTATAATTTT TGTGTGCCTG 
     3301 GGGATCAAAT GAATCCAGAA TTTAATTTTT TCACATCTGT TGATCATTGT CCAGCCACAC 
     3361 AAGATAACCT CAACAAAGAT AGCATGCTAC TGGCTAGCAT TTTAACTCCC AGCGTTGAAG 
     3421 CAGATAAGAA GATTCTTAAA CAGGTAAGTA TTACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3481 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3541 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3601 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3661 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     3721 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     3781 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     3841 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     3901 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     3961 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4021 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4081 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4141 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4201 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4261 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4321 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4381 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4441 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4501 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4561 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4621 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4681 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4741 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4801 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     4861 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     4921 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4981 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5041 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5101 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5161 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5221 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5281 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5341 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5401 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5461 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5521 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5581 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5641 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     5701 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     5761 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     5821 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     5881 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     5941 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6001 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6061 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6121 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6181 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6241 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6301 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6361 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6421 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6481 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6541 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6601 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6661 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     6721 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     6781 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     6841 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     6901 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     6961 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7021 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7081 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7141 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7201 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7261 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7321 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7381 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7441 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7501 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7561 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7621 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7681 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     7741 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     7801 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     7861 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     7921 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     7981 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8041 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8101 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8161 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8221 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8281 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8341 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8401 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8461 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8521 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8581 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8641 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     8701 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     8761 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     8821 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     8881 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     8941 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//