LOCUS       RG221956                9272 bp    DNA     circular  SYN 
DEFINITION  ERMP1 (tGFP-tagged) - Human endoplasmic reticulum metallopeptidase 1 (ERMP1)
ACCESSION   NM_024896
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3753..4457 
                     /label="GFP"  
     misc_feature    4504..5089 
                     /label="PolyA signal"  
     misc_feature    7181..7826 
                     /label="ColE1"  
     misc_feature    5543..6337 
                     /label="Neo"  
     misc_feature    5174..5476 
                     /label="SV40 ori"  
     misc_feature    7841..8701 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3732..3738 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3732 
                     /label="ERMP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTGGGGT TCTGAGTCGG CTGCTGTGAG GCGGCACCGC GTCGGAGTAG 
     1081 AGCGTCGAGA GGGAGCGGCG GCCGCGCCAC CGCCGGAGAG GGAGGCCCGA GCGCAGGAGC 
     1141 CTCTGGTGGA TGGGTGCAGC GGCGGCGGGA GGACGCGGAA GAGGAGCCCC GGGGGTAGCG 
     1201 GCGGCGCGAG CAGGGGCGCG GGGACCGGGC TGTCTGAGGT GCGCGCCGCG CTGGGGCTCG 
     1261 CGCTCTACCT GATCGCGCTG CGGACGCTGG TGCAGCTCTC GCTGCAGCAG CTCGTGCTAC 
     1321 GCGGGGCCGC TGGACACCGC GGGGAGTTCG ACGCGCTCCA AGCCAGGGAT TATCTTGAAC 
     1381 ACATAACCTC CATTGGCCCC AGGACTACAG GAAGTCCAGA AAATGAAATT CTGACCGTGC 
     1441 ACTACCTTTT GGAACAGATT AAACTGATTG AAGTGCAAAG CAACAGCCTT CATAAGATTT 
     1501 CAGTAGATGT ACAACGGCCC ACAGGCTCTT TTAGCATTGA TTTCTTGGGA GGTTTTACAA 
     1561 GCTATTATGA CAACATCACC AATGTTGTGG TAAAGCTGGA ACCCAGAGAT GGAGCCCAGC 
     1621 ATGCTGTCTT GGCTAATTGT CATTTTGACT CAGTAGCAAA CTCACCAGGT GCCAGTGATG 
     1681 ATGCAGTTAG CTGCTCAGTG ATGCTGGAAG TCCTTCGCGT CTTGTCAACA TCTTCAGAAG 
     1741 CCTTGCATCA TGCTGTCATA TTTCTCTTTA ATGGTGCTGA GGAAAATGTC TTGCAAGCCA 
     1801 GTCATGGTTT CATTACTCAG CACCCCTGGG CTAGCTTGAT TCGTGCATTC ATTAACCTAG 
     1861 AGGCAGCAGG TGTAGGAGGG AAAGAACTTG TATTCCAAAC AGGTCCTGAA AATCCTTGGT 
     1921 TGGTTCAAGC TTATGTTTCA GCAGCTAAAC ACCCTTTTGC TTCTGTGGTG GCTCAGGAGG 
     1981 TTTTTCAGAG TGGAATCATT CCTTCAGATA CTGACTTTCG TATCTACAGG GATTTTGGGA 
     2041 ACATTCCAGG AATAGACTTA GCTTTTATTG AGAATGGATA CATTTATCAC ACCAAGTATG 
     2101 ACACAGCGGA CAGAATTCTA ACAGATTCCA TTCAGAGAGC AGGTGACAAC ATTTTAGCAG 
     2161 TTCTTAAGCA TCTAGCTACA TCTGATATGC TGGCTGCTGC TTCTAAGTAT CGACATGGAA 
     2221 ACATGGTCTT CTTTGATGTG CTGGGCCTGT TTGTCATTGC CTACCCCTCT CGTATTGGCT 
     2281 CAATCATAAA CTACATGGTG GTAATGGGTG TTGTTTTGTA CCTGGGCAAA AAATTTTTGC 
     2341 AGCCCAAACA TAAGACTGGT AACTACAAGA AGGACTTCTT GTGTGGACTT GGCATCACTT 
     2401 TGATCAGCTG GTTCACTAGC CTTGTTACCG TTCTCATTAT AGCAGTGTTC ATCTCTCTTA 
     2461 TTGGACAGTC TCTCTCATGG TATAACCACT TCTATGTCTC CGTTTGTCTG TATGGAACTG 
     2521 CAACTGTAGC CAAAATAATA CTTATACATA CTCTTGCGAA AAGATTTTAT TACATGAATG 
     2581 CCAGTGCCCA GTATCTGGGA GAAGTATTTT TTGACATTTC GCTGTTTGTC CATTGCTGTT 
     2641 TTCTTGTTAC CCTCACTTAC CAAGGACTTT GCTCGGCGTT TATTAGTGCT GTCTGGGTAG 
     2701 CATTCCCATT GCTCACAAAG CTCTGTGTGC ATAAGGACTT CAAGCAGCAT GGTGCCCAAG 
     2761 GAAAATTTAT TGCTTTTTAC CTTTTGGGGA TGTTTATTCC TTATCTTTAT GCATTGTACC 
     2821 TCATCTGGGC AGTATTTGAG ATGTTTACCC CTATCCTCGG GAGAAGTGGT TCTGAAATCC 
     2881 CACCTGATGT TGTGCTGGCA TCCATTTTGG CTGGCTGTAC AATGATTCTC TCGTCCTATT 
     2941 TTATTAACTT CATCTACCTT GCCAAGAGCA CAAAAAAAAC CATGCTAACT TTAACTTTGG 
     3001 TATGTGCAAT TACATTCCTC CTTGTTTGCA GTGGAACATT TTTTCCATAT AGCTCCAATC 
     3061 CTGCTAATCC GAAGCCAAAG AGAGTGTTTC TTCAGCATAT GACTAGAACA TTCCATGACT 
     3121 TGGAAGGAAA TGCAGTTAAA CGGGACTCTG GAATATGGAT CAATGGGTTT GATTATACTG 
     3181 GAATTTCTCA CATAACCCCT CACATTCCTG AGATCAATGA TAGTATCCGA GCTCACTGTG 
     3241 AGGAGAATGC ACCTCTTTGT GGTTTTCCTT GGTATCTTCC AGTGCACTTT CTGATCAGGA 
     3301 AAAACTGGTA TCTTCCTGCC CCAGAAGTTT CTCCAAGAAA TCCTCCTCAT TTCCGACTCA 
     3361 TATCCAAAGA ACAGACACCT TGGGATTCTA TAAAATTGAC TTTTGAAGCA ACAGGACCAA 
     3421 GCCATATGTC CTTCTATGTT CGAGCCCACA AAGGGTCAAC ACTTTCTCAG TGGTCTCTTG 
     3481 GCAATGGCAC CCCAGTCACA AGTAAAGGAG GAGACTACTT TGTCTTTTAC TCCCATGGAC 
     3541 TCCAGGCCTC TGCATGGCAG TTCTGGATAG AAGTGCAGGT TTCAGAAGAA CATCCTGAAG 
     3601 GAATGGTCAC CGTGGCCATT GCTGCCCACT ATCTGTCTGG GGAAGACAAG AGATCCCCTC 
     3661 AACTGGATGC TCTGAAGGAA AAGTTCCCAG ATTGGACATT TCCCTCTGCC TGGGTGTGCA 
     3721 CCTACGATCT CTTTGTATTT ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3781 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3841 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3901 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3961 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     4021 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4081 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4141 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4201 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4261 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4321 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4381 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4441 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4501 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4561 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4621 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4681 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4741 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4801 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4861 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4921 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4981 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5041 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5101 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5161 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5221 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5281 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5341 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5401 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5461 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5521 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5581 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5641 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5701 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5761 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5821 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5881 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5941 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     6001 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6061 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6121 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6181 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6241 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6301 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6361 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6421 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6481 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6541 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6601 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6661 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6721 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6781 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6841 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6901 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6961 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     7021 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7081 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7141 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7201 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7261 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7321 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7381 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7441 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7501 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7561 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7621 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7681 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7741 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7801 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7861 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7921 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7981 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     8041 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8101 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8161 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8221 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8281 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8341 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8401 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8461 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8521 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8581 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8641 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8701 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8761 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8821 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8881 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8941 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     9001 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9061 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9121 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9181 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9241 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//