LOCUS       RG221638                9206 bp    DNA     circular  SYN 
DEFINITION  NDST1 (tGFP-tagged) - Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1)
ACCESSION   NM_001543
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3687..4391 
                     /label="GFP"  
     misc_feature    4438..5023 
                     /label="PolyA signal"  
     misc_feature    7115..7760 
                     /label="ColE1"  
     misc_feature    5477..6271 
                     /label="Neo"  
     misc_feature    5108..5410 
                     /label="SV40 ori"  
     misc_feature    7775..8635 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3666..3672 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3666 
                     /label="NDST1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGCCCTG GCATGCCTCC GGAGGCTGTG TCGGCACGTG TCCCCGCAGG 
     1081 CTGTCCTTTT CCTGCTGTTC ATCTTCTGCC TGTTCAGCGT TTTCATCTCG GCCTACTACC 
     1141 TATATGGCTG GAAGCGAGGC CTGGAGCCCT CGGCGGATGC CCCCGAGCCT GACTGCGGGG 
     1201 ACCCGCCGCC TGTGGCCCCC AGTCGCCTGC TGCCACTCAA GCCTGTGCAG GCAGCCACCC 
     1261 CTTCCCGCAC AGACCCGTTG GTGCTGGTCT TTGTGGAGAG CCTCTACTCG CAACTGGGCC 
     1321 AGGAGGTGGT GGCCATCCTG GAGTCCAGCC GCTTCAAATA CCGCACAGAG ATTGCGCCGG 
     1381 GCAAGGGTGA CATGCCCACG CTCACTGACA AGGGCCGTGG CCGCTTCGCC CTCATCATCT 
     1441 ATGAGAACAT CCTCAAGTAT GTCAACCTGG ACGCCTGGAA CCGGGAGCTG CTGGACAAGT 
     1501 ACTGTGTGGC CTACGGCGTG GGCATCATTG GCTTCTTCAA GGCCAATGAG AACAGCCTGC 
     1561 TGAGTGCGCA GCTCAAGGGC TTCCCCCTGT TCCTGCACTC AAACCTGGGC CTGAAGGACT 
     1621 GCAGCATCAA CCCCAAGTCC CCGCTGCTCT ACGTGACGCG ACCTAGCGAG GTGGAGAAAG 
     1681 GTGTGCTCCC CGGCGAGGAC TGGACGGTTT TCCAGTCAAA TCACTCCACC TATGAGCCAG 
     1741 TGCTGCTGGC CAAGACGCGC TCGTCTGAGT CCATCCCACA CCTGGGCGCA GACGCCGGCC 
     1801 TGCATGCTGC ACTGCACGCC ACTGTGGTCC AGGACCTGGG CCTGCACGAC GGCATCCAGC 
     1861 GCGTGCTGTT TGGCAACAAC CTGAACTTCT GGCTGCACAA GCTTGTCTTC GTGGATGCCG 
     1921 TGGCCTTCCT CACGGGGAAG CGCCTCTCCC TGCCATTGGA CCGCTACATC CTGGTGGACA 
     1981 TTGATGACAT CTTCGTGGGC AAGGAGGGCA CACGCATGAA GGTGGAGGAC GTGAAGGCCC 
     2041 TGTTTGACAC ACAGAACGAA CTACGCGCAC ACATCCCAAA CTTCACCTTC AACCTGGGCT 
     2101 ACTCAGGGAA ATTCTTCCAC ACAGGTACCA ATGCTGAGGA CGCTGGGGAT GATCTGCTGC 
     2161 TGTCGTATGT GAAGGAGTTC TGGTGGTTCC CCCACATGTG GAGCCACATG CAGCCCCACC 
     2221 TTTTCCACAA CCAGTCCGTG TTGGCCGAGC AGATGGCCTT GAACAAGAAG TTCGCTGTCG 
     2281 AGCATGGCAT TCCCACAGAC ATGGGGTATG CAGTGGCGCC CCACCACTCG GGCGTGTACC 
     2341 CCGTGCACGT GCAGCTGTAC GAGGCTTGGA AGCAGGTGTG GAGCATCCGC GTGACCAGCA 
     2401 CGGAGGAGTA CCCCCACCTG AAGCCAGCCC GCTACCGCCG TGGCTTCATC CACAATGGCA 
     2461 TCATGGTTCT CCCACGGCAG ACCTGCGGCC TCTTCACACA CACCATCTTC TACAACGAGT 
     2521 ACCCTGGCGG CTCCAGTGAG CTGGACAAGA TCATCAACGG GGGCGAGCTC TTCCTCACCG 
     2581 TGCTCCTCAA TCCTATCAGC ATCTTCATGA CGCACCTGTC CAACTATGGG AATGACCGCC 
     2641 TGGGCCTGTA CACCTTCAAG CACCTGGTGC GCTTCCTGCA CTCCTGGACG AACCTCCGGC 
     2701 TGCAGACACT GCCCCCTGTG CAGTTGGCGC AGAAGTACTT CCAGATCTTC TCCGAGGAGA 
     2761 AGGACCCGCT CTGGCAGGAC CCCTGCGAGG ACAAACGTCA CAAAGACATC TGGTCCAAGG 
     2821 AGAAGACGTG TGACCGCTTC CCAAAGCTCC TCATCATCGG CCCCCAGAAA ACAGGCACCA 
     2881 CTGCCCTCTA CCTGTTCCTG GGCATGCACC CTGACCTAAG CAGCAACTAC CCCAGCTCTG 
     2941 AGACCTTTGA GGAGATCCAG TTTTTTAATG GCCACAACTA TCACAAAGGC ATCGACTGGT 
     3001 ACATGGAGTT CTTCCCCATC CCTTCCAACA CCACCTCCGA CTTCTACTTT GAGAAAAGCG 
     3061 CCAACTACTT TGATTCAGAA GTGGCGCCCC GGCGGGCAGC AGCCCTCTTG CCCAAAGCCA 
     3121 AGGTCCTGAC CATCCTCATC AACCCCGCGG ACCGGGCCTA TTCCTGGTAC CAGCACCAGC 
     3181 GAGCCCATGA CGACCCAGTG GCCCTAAAGT ACACCTTCCA TGAGGTGATT ACCGCCGGCT 
     3241 CTGACGCATC CTCGAAGCTG CGTGCCCTCC AGAACCGCTG CCTGGTCCCT GGCTGGTACG 
     3301 CCACCCACAT CGAGCGCTGG CTCAGTGCCT ATCACGCCAA CCAGATTCTG GTCTTGGATG 
     3361 GCAAACTGCT TCGCACAGAA CCTGCCAAAG TGATGGACAT GGTGCAGAAG TTCCTTGGGG 
     3421 TGACCAACAC CATTGACTAC CACAAAACCT TGGCGTTTGA TCCAAAGAAA GGATTTTGGT 
     3481 GCCAACTGCT TGAAGGAGGA AAAACCAAGT GTCTGGGCAA AAGCAAGGGC CGGAAATATC 
     3541 CCGAGATGGA CTTGGATTCC CGAGCCTTCC TGAAGGACTA TTACCGGGAC CACAACATCG 
     3601 AGCTCTCCAA GCTGCTGTAT AAGATGGGCC AGACACTTCC CACTTGGCTA CGAGAGGACC 
     3661 TCCAGAACAC CAGGACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3721 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3781 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3841 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     3901 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     3961 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     4021 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     4081 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4141 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4201 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4261 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4321 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4381 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4441 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4501 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4561 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4621 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4681 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4741 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4801 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4861 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4921 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4981 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5041 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     5101 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5161 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5221 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5281 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5341 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5401 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5461 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5521 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5581 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5641 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5701 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5761 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5821 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     5881 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     5941 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     6001 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     6061 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6121 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6181 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6241 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6301 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6361 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6421 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6481 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6541 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6601 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6661 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6721 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6781 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6841 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     6901 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     6961 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     7021 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7081 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7141 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7201 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7261 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7321 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7381 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7441 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7501 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7561 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7621 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7681 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7741 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7801 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7861 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     7921 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     7981 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     8041 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     8101 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8161 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8221 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8281 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8341 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8401 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8461 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8521 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8581 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8641 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8701 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8761 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8821 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     8881 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     8941 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     9001 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     9061 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9121 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9181 TTTAACAAAA ATTTAACGCG AATTTT
//