LOCUS       RG221606                8933 bp    DNA     circular  SYN 
DEFINITION  ITGB3 (tGFP-tagged) - Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3)
ACCESSION   NM_000212
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3412..4116 
                     /label="GFP"  
     misc_feature    4163..4748 
                     /label="PolyA signal"  
     misc_feature    6840..7485 
                     /label="ColE1"  
     misc_feature    5202..5996 
                     /label="Neo"  
     misc_feature    4833..5135 
                     /label="SV40 ori"  
     misc_feature    7500..8360 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3384..3391 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3384 
                     /label="ITGB3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGAGCGCGG CCGCGGCCCC GGCCGCTCTG GGCGACTGTG CTGGCGCTGG 
     1081 GGGCGCTGGC GGGCGTTGGC GTAGGAGGGC CCAACATCTG TACCACGCGA GGTGTGAGCT 
     1141 CCTGCCAGCA GTGCCTGGCT GTGAGCCCCA TGTGTGCCTG GTGCTCTGAT GAGGCCCTGC 
     1201 CTCTGGGCTC ACCTCGCTGT GACCTGAAGG AGAATCTGCT GAAGGATAAC TGTGCCCCAG 
     1261 AATCCATCGA GTTCCCAGTG AGTGAGGCCC GAGTACTAGA GGACAGGCCC CTCAGCGACA 
     1321 AGGGCTCTGG AGACAGCTCC CAGGTCACTC AAGTCAGTCC CCAGAGGATT GCACTCCGGC 
     1381 TCCGGCCAGA TGATTCGAAG AATTTCTCCA TCCAAGTGCG GCAGGTGGAG GATTACCCTG 
     1441 TGGACATCTA CTACTTGATG GACCTGTCTT ACTCCATGAA GGATGATCTG TGGAGCATCC 
     1501 AGAACCTGGG TACCAAGCTG GCCACCCAGA TGCGAAAGCT CACCAGTAAC CTGCGGATTG 
     1561 GCTTCGGGGC ATTTGTGGAC AAGCCTGTGT CACCATACAT GTATATCTCC CCACCAGAGG 
     1621 CCCTCGAAAA CCCCTGCTAT GATATGAAGA CCACCTGCTT GCCCATGTTT GGCTACAAAC 
     1681 ACGTGCTGAC GCTAACTGAC CAGGTGACCC GCTTCAATGA GGAAGTGAAG AAGCAGAGTG 
     1741 TGTCACGGAA CCGAGATGCC CCAGAGGGTG GCTTTGATGC CATCATGCAG GCTACAGTCT 
     1801 GTGATGAAAA GATTGGCTGG AGGAATGATG CATCCCACTT GCTGGTGTTT ACCACTGATG 
     1861 CCAAGACTCA TATAGCATTG GACGGAAGGC TGGCAGGCAT TGTCCAGCCT AATGACGGGC 
     1921 AGTGTCATGT TGGTAGTGAC AATCATTACT CTGCCTCCAC TACCATGGAT TATCCCTCTT 
     1981 TGGGGCTGAT GACTGAGAAG CTATCCCAGA AAAACATCAA TTTGATCTTT GCAGTGACTG 
     2041 AAAATGTAGT CAATCTCTAT CAGAACTATA GTGAGCTCAT CCCAGGGACC ACAGTTGGGG 
     2101 TTCTGTCCAT GGATTCCAGC AATGTCCTCC AGCTCATTGT TGATGCTTAT GGGAAAATCC 
     2161 GTTCTAAAGT AGAGCTGGAA GTGCGTGACC TCCCTGAAGA GTTGTCTCTA TCCTTCAATG 
     2221 CCACCTGCCT CAACAATGAG GTCATCCCTG GCCTCAAGTC TTGTATGGGA CTCAAGATTG 
     2281 GAGACACGGT GAGCTTCAGC ATTGAGGCCA AGGTGCGAGG CTGTCCCCAG GAGAAGGAGA 
     2341 AGTCCTTTAC CATAAAGCCC GTGGGCTTCA AGGACAGCCT GATCGTCCAG GTCACCTTTG 
     2401 ATTGTGACTG TGCCTGCCAG GCCCAAGCTG AACCTAATAG CCATCGCTGC AACAATGGCA 
     2461 ATGGGACCTT TGAGTGTGGG GTATGCCGTT GTGGGCCTGG CTGGCTGGGA TCCCAGTGTG 
     2521 AGTGCTCAGA GGAGGACTAT CGCCCTTCCC AGCAGGACGA ATGCAGCCCC CGGGAGGGTC 
     2581 AGCCCGTCTG CAGCCAGCGG GGCGAGTGCC TCTGTGGTCA ATGTGTCTGC CACAGCAGTG 
     2641 ACTTTGGCAA GATCACGGGC AAGTACTGCG AGTGTGACGA CTTCTCCTGT GTCCGCTACA 
     2701 AGGGGGAGAT GTGCTCAGGC CATGGCCAGT GCAGCTGTGG GGACTGCCTG TGTGACTCCG 
     2761 ACTGGACCGG CTACTACTGC AACTGTACCA CGCGTACTGA CACCTGCATG TCCAGCAATG 
     2821 GGCTGCTGTG CAGCGGCCGC GGCAAGTGTG AATGTGGCAG CTGTGTCTGT ATCCAGCCGG 
     2881 GCTCCTATGG GGACACCTGT GAGAAGTGCC CCACCTGCCC AGATGCCTGC ACCTTTAAGA 
     2941 AAGAATGTGT GGAGTGTAAG AAGTTTGACC GGGGAGCCCT ACATGACGAA AATACCTGCA 
     3001 ACCGTTACTG CCGTGACGAG ATTGAGTCAG TGAAAGAGCT TAAGGACACT GGCAAGGATG 
     3061 CAGTGAATTG TACCTATAAG AATGAGGATG ACTGTGTCGT CAGATTCCAG TACTATGAAG 
     3121 ATTCTAGTGG AAAGTCCATC CTGTATGTGG TAGAAGAGCC AGAGTGTCCC AAGGGCCCTG 
     3181 ACATCCTGGT GGTCCTGCTC TCAGTGATGG GGGCCATTCT GCTCATTGGC CTTGCCGCCC 
     3241 TGCTCATCTG GAAACTCCTC ATCACCATCC ACGACCGAAA AGAATTCGCT AAATTTGAGG 
     3301 AAGAACGCGC CAGAGCAAAA TGGGACACAG CCAACAACCC ACTGTATAAA GAGGCCACGT 
     3361 CTACCTTCAC CAATATCACG TACCGGGGCA CTAGCGGACC GACGCGTACG CGGCCGCTCG 
     3421 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3481 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     3541 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     3601 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     3661 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     3721 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     3781 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     3841 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     3901 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     3961 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4021 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4081 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4141 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4201 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4261 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4321 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4381 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4441 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4501 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4561 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4621 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4681 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4741 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4801 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     4861 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     4921 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     4981 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5041 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5101 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5161 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5221 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5281 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5341 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5401 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5461 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     5521 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     5581 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     5641 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     5701 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     5761 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     5821 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     5881 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     5941 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6001 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6061 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6121 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6181 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6241 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6301 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6361 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6421 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6481 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     6541 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     6601 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     6661 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     6721 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     6781 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     6841 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     6901 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     6961 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7021 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7081 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7141 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7201 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7261 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7321 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7381 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7441 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7501 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     7561 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     7621 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     7681 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     7741 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     7801 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     7861 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     7921 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     7981 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8041 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8101 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8161 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8221 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8281 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8341 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8401 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8461 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     8521 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     8581 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     8641 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     8701 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     8761 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     8821 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     8881 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//