LOCUS       RG221507                9380 bp    DNA     circular  SYN 
DEFINITION  PPP1R10 (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 10 (PPP1R10)
ACCESSION   NM_002714
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3861..4565 
                     /label="GFP"  
     misc_feature    4612..5197 
                     /label="PolyA signal"  
     misc_feature    7289..7934 
                     /label="ColE1"  
     misc_feature    5651..6445 
                     /label="Neo"  
     misc_feature    5282..5584 
                     /label="SV40 ori"  
     misc_feature    7949..8809 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3840..3846 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3840 
                     /label="PPP1R10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTTCGGGT CCCATAGACC CCAAAGAACT TCTCAAGGGC CTGGACAGCT 
     1081 TCCTTAACCG AGATGGGGAA GTCAAAAGTG TGGATGGGAT TTCCAAGATC TTCAGTTTGA 
     1141 TGAAGGAAGC ACGAAAGATG GTGAGTCGAT GCACTTACTT GAACATTCTC CTGCAGACCC 
     1201 GTTCACCAGA AATATTGGTC AAATTTATTG ACGTTGGCGG CTACAAACTT CTTAACAATT 
     1261 GGCTGACGTA TTCAAAGACA ACCAACAACA TTCCCCTCCT CCAGCAAATT CTACTGACCC 
     1321 TGCAGCATCT ACCGCTCACT GTAGACCATC TCAAGCAGAA CAACACAGCT AAACTGGTGA 
     1381 AGCAGCTGAG CAAGTCAAGT GAGGATGAAG AGCTCCGGAA ATTGGCCTCA GTCCTTGTCA 
     1441 GCGACTGGAT GGCTGTCATC CGCTCTCAGA GCAGTACCCA GCCTGCTGAG AAAGATAAGA 
     1501 AGAAACGTAA AGATGAAGGA AAAAGTCGAA CTACCCTTCC TGAGCGACCT TTGACAGAGG 
     1561 TGAAGGCTGA GACCCGGGCT GAGGAGGCCC CAGAGAAGAA GAGGGAGAAG CCCAAGTCTC 
     1621 TTCGCACCAC AGCACCCAGT CATGCCAAGT TCCGTTCCAC TGGACTAGAG CTGGAGACAC 
     1681 CATCCTTGGT GCCTGTGAAG AAGAATGCCA GCACAGTGGT GGTTTCTGAC AAGTACAACC 
     1741 TTAAACCCAT CCCCCTCAAA CGTCAGAGCA ACGTAGCTGC TCCAGGAGAT GCCACTCCCC 
     1801 CTGCAGAGAA GAAATACAAG CCACTCAACA CAACACCTAA TGCCACCAAA GAGATCAAAG 
     1861 TGAAGATCAT CCCGCCACAG CCTATGGAGG GCCTGGGCTT TCTGGATGCT CTTAATTCAG 
     1921 CCCCTGTTCC AGGCATCAAA ATTAAGAAGA AAAAAAAAGT ACTGTCACCT ACGGCTGCCA 
     1981 AGCCAAGCCC CTTTGAAGGG AAAACGAGCA CAGAACCAAG CACAGCCAAA CCTTCTTCCC 
     2041 CAGAACCAGC ACCACCTTCT GAGGCAATGG ACGCAGACCG TCCAGGCACC CCGGTTCCCC 
     2101 CTGTTGAAGT CCCGGAGCTC ATGGATACAG CCTCTTTGGA GCCAGGAGCT CTGGATGCCA 
     2161 AGCCAGTGGA GAGTCCTGGA GATCCTAACC AACTGACCCG GAAAGGCAGG AAGAGGAAAA 
     2221 GTGTGACATG GCCTGAGGAA GGCAAACTGA GAGAATATTT CTATTTTGAA TTGGATGAAA 
     2281 CTGAACGAGT AAATGTGAAT AAGATCAAGG ACTTTGGTGA GGCGGCTAAG CGAGAGATAC 
     2341 TGTCAGACCG ACATGCATTT GAGACAGCGC GGCGTCTGAG CCATGATAAC ATGGAGGAGA 
     2401 AGGTGCCCTG GGTGTGCCCC CGGCCCCTGG TTCTGCCCTC ACCTCTTGTC ACCCCTGGAA 
     2461 GCAATAGTCA GGAGCGATAT ATCCAGGCTG AGCGGGAGAA GGGAATCCTT CAGGAGCTCT 
     2521 TCCTGAACAA GGAGAGTCCT CATGAGCCTG ATCCTGAGCC CTACGAGCCC ATACCCCCTA 
     2581 AACTCATCCC CCTAGATGAG GAGTGTTCCA TGGATGAGAC TCCGTATGTT GAGACTCTGG 
     2641 AACCTGGGGG GTCAGGTGGC TCACCTGATG GGGCAGGAGG CTCCAAGTTG CCTCCAGTTC 
     2701 TGGCCAATCT TATGGGAAGC ATGGGTGCTG GAAAGGGCCC CCAAGGCCCT GGAGGAGGAG 
     2761 GCATTAATGT CCAAGAGATC CTCACCTCCA TCATGGGTAG CCCAAACAGT CATCCTTCAG 
     2821 AGGAACTACT GAAACAACCA GACTATTCGG ACAAGATCAA GCAGATGCTG GTGCCACATG 
     2881 GACTCCTAGG CCCTGGCCCA ATAGCCAATG GTTTCCCACC AGGGGGTCCT GGGGGCCCCA 
     2941 AGGGCATGCA GCACTTTCCC CCTGGACCTG GGGGACCTAT GCCAGGTCCC CATGGAGGCC 
     3001 CTGGTGGGCC AGTGGGTCCA CGTCTTCTGG GTCCTCCACC CCCTCCCCGG GGAGGTGATC 
     3061 CCTTCTGGGA TGGCCCGGGC GACCCTATGC GGGGTGGCCC AATGCGGGGG GGTCCAGGAC 
     3121 CAGGTCCTGG ACCATACCAT AGAGGCCGAG GTGGCCGAGG AGGAAACGAA CCTCCTCCTC 
     3181 CTCCTCCTCC ATTCCGAGGC GCCAGAGGAG GTCGCTCTGG AGGAGGACCC CCAAATGGAC 
     3241 GAGGGGGCCC TGGTGGGGGC ATGGTTGGAG GTGGTGGGCA TCGTCCTCAC GAAGGCCCTG 
     3301 GTGGGGGCAT GGGCAACAGC AGTGGACATC GTCCCCACGA AGGCCCTGGC GGTGGCATGG 
     3361 GAAGTGGGCA TCGCCCCCAT GAAGGCCCTG GTGGTAGCAT GGGTGGGGGT GGAGGACATC 
     3421 GTCCCCACGA AGGCCCTGGC GGTGGCATCA GTGGTGGCAG TGGCCATCGT CCCCATGAAG 
     3481 GCCCTGGCGG AGGAATGGGT GCCGGTGGTG GACATCGCCC CCACGAAGGC CCTGGCGGAA 
     3541 GCATGGGTGG AAGTGGTGGA CATCGTCCCC ATGAAGGCCC TGGACACGGG GGGCCCCATG 
     3601 GCCACCGGCC TCATGATGTC CCTGGTCACC GAGGCCATGA CCATCGAGGG CCGCCACCTC 
     3661 ATGAGCACCG TGGCCATGAT GGTCCTGGCC ACGGGGGAGG GGGCCACCGA GGGCACGATG 
     3721 GAGGCCACAG CCATGGAGGA GACATGTCAA ACCGCCCTGT CTGCCGACAT TTCATGATGA 
     3781 AGGGCAACTG CCGCTATGAG AACAACTGTG CCTTCTACCA CCCGGGTGTC AATGGGCCCC 
     3841 CCCTGCCCAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3901 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3961 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     4021 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     4081 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     4141 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     4201 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     4261 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4321 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4381 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4441 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4501 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4561 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     5281 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5341 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5401 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5461 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5521 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5581 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5641 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5701 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5761 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5821 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5881 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5941 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     6001 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     6061 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     6121 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     6181 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     6241 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6301 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6361 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6421 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6481 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6541 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6601 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6661 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6721 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6781 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6841 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6901 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6961 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     7021 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     7081 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     7141 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     7201 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     7261 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7321 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7381 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7441 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7501 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7561 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7621 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7681 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7741 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7801 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7861 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7921 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7981 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     8041 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     8101 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     8161 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     8221 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     8281 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8341 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8401 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8461 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8521 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8581 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8641 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8701 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8761 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8821 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8881 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8941 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     9001 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     9061 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     9121 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     9181 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     9241 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9301 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9361 AAAAATTTAA CGCGAATTTT 
//