LOCUS       RG221384                9419 bp    DNA     circular  SYN 
DEFINITION  SAFB2 (tGFP-tagged) - Human scaffold attachment factor B2 (SAFB2)
ACCESSION   NM_014649
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3900..4604 
                     /label="GFP"  
     misc_feature    4651..5236 
                     /label="PolyA signal"  
     misc_feature    7328..7973 
                     /label="ColE1"  
     misc_feature    5690..6484 
                     /label="Neo"  
     misc_feature    5321..5623 
                     /label="SV40 ori"  
     misc_feature    7988..8848 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3879..3885 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3879 
                     /label="SAFB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGACT CTGCCCGGGT CGGGCGACTC GGGCCCTGGC ACGGCTTCTC 
     1081 TCGGCCCGGG CGTTGCGGAG ACTGGGACGA GGCGGCTCAG CGAGCTGCGG GTGATCGATC 
     1141 TGCGGGCGGA GCTGAAGAAG CGGAACCTGG ACACGGGCGG CAACAAGAGC GTCCTGATGG 
     1201 AGCGGCTCAA GAAGGCGGTT AAAGAAGAGG GGCAAGATCC TGATGAAATT GGCATCGAGT 
     1261 TAGAAGCCAC CAGCAAGAAG TCAGCCAAGA GATGTGTTAA AGGACTGAAG ATGGAGGAGG 
     1321 AAGGCACAGA AGATAATGGC CTGGAAGACG ATTCCAGAGA CGGGCAGGAG GACATGGAAG 
     1381 CAAGTCTGGA GAACCTGCAG AATATGGGCA TGATGGACAT GAGTGTGCTA GACGAAACTG 
     1441 AAGTGGCGAA TAGCAGTGCT CCAGATTTTG GGGAGGATGG CACGGACGGC CTTCTCGATT 
     1501 CCTTTTGTGA TAGTAAAGAA TACGTGGCTG CACAGCTGAG ACAGCTCCCG GCTCAGCCCC 
     1561 CAGAGCATGC TGTGGATGGG GAAGGATTTA AGAACACTTT GGAAACTTCA TCGTTGAACT 
     1621 TCAAAGTAAC TCCGGACATT GAAGAATCCC TTTTGGAGCC AGAAAATGAG AAAATACTCG 
     1681 ACATTTTGGG GGAAACTTGT AAATCTGAGC CAGTAAAAGA AGAAAGTTCC GAGCTGGAGC 
     1741 AGCCATTTGC ACAGGACACA AGTAGCGTGG GGCCAGACAG AAAGCTTGCG GAGGAAGAGG 
     1801 ACCTATTTGA CAGCGCCCAT CCGGAAGAGG GTGATTTAGA TTTGGCCAGC GAGTCAACAG 
     1861 CACACGCTCA GTCGAGCAAG GCAGACAGCC TGTTAGCGGT AGTGAAAAGG GAGCCCGCGG 
     1921 AGCAGCCAGG CGATGGCGAG AGGACGGACT GTGAGCCTGT AGGGCTAGAG CCGGCAGTTG 
     1981 AGCAGAGTAG TGCGGCCTCC GAGCTCGCGG AGGCCTCTAG CGAGGAGCTC GCAGAAGCAC 
     2041 CCACGGAAGC CCCAAGCCCA GAAGCCAGAG ATAGCAAAGA AGACGGGAGG AAGTTTGATT 
     2101 TTGACGCTTG TAATGAAGTC CCTCCGGCTC CTAAAGAGTC CTCAACCAGT GAGGGCGCTG 
     2161 ATCAGAAAAT GAGCTCTTTT AAGGAAGAAA AAGATATAAA GCCAATCATT AAAGATGAAA 
     2221 AAGGTCGGGT CGGCAGCGGT TCTGGTCGGA ACCTGTGGGT CAGCGGGCTG TCCTCCACAA 
     2281 CACGCGCTAC GGATCTCAAG AACCTTTTCA GCAAGTATGG GAAGGTTGTC GGGGCCAAAG 
     2341 TGGTAACGAA CGCCCGCAGC CCGGGGGCTC GATGCTATGG ATTCGTCACC ATGTCGACAT 
     2401 CTGACGAGGC GACCAAGTGC ATCAGCCATC TCCACAGAAC TGAGCTGCAT GGACGAATGA 
     2461 TCTCCGTAGA GAAGGCCAAA AATGAGCCTG CTGGGAAAAA GCTTTCCGAC AGAAAAGAGT 
     2521 GCGAAGTGAA GAAGGAAAAA TTATCGAGTG TCGACAGACA TCATTCTGTG GAGATCAAAA 
     2581 TTGAAAAAAC TGTAATTAAG AAGGAAGAGA AGATTGAGAA GAAGGAGGAA AAAAAGCCTG 
     2641 AAGACATTAA GAAGGAAGAA AAAGACCAGG ATGAGCTGAA ACCCGGACCT ACAAATCGGT 
     2701 CTAGAGTCAC CAAATCAGGA AGCAGAGGAA TGGAGCGGAC GGTCGTGATG GATAAATCGA 
     2761 AAGGAGAGCC CGTCATTAGC GTGAAAACCA CAAGCAGGTC CAAAGAGAGA AGCTCCAAGA 
     2821 GTCAGGATCG CAAGTCAGAA AGCAAAGAAA AGAGAGACAT CTTGTCGTTT GATAAAATCA 
     2881 AAGAACAAAG GGAGAGAGAG CGCCAGAGGC AGCGGGAACG GGAGATCCGC GAAACGGAGA 
     2941 GGCGGCGGGA GCGCGAGCAG CGGGAGCGGG AGCAACGCCT CGAGGCCTTC CATGAGCGGA 
     3001 AGGAGAAGGC CCGGCTACAG CGGGAACGCC TGCAGCTCGA GTGCCAGCGC CAGCGGCTGG 
     3061 AGCGGGAGCG CATGGAGCGG GAGCGGCTGG AGCGCGAGCG CATGCGCGTG GAGCGTGAGC 
     3121 GCAGGAAGGA GCAGGAGCGC ATCCACCGCG AGCGCGAGGA GCTGCGGCGC CAGCAGGAGC 
     3181 AGCTGCGTTA CGAGCAGGAG CGGCGGCCCG GGCGGAGGCC CTACGACCTG GACCGACGAG 
     3241 ATGATGCCTA TTGGCCAGAA GGAAAGCGTG TGGCAATGGA GGACCGATAT CGTGCAGACT 
     3301 TTCCCCGGCC AGACCACCGC TTTCACGACT TCGATCATCG AGACCGGGGC CAGTACCAGG 
     3361 ACCACGCCAT CGACAGGCGG GAGGGTTCGA GGCCAATGAT GGGAGACCAC CGGGATGGGC 
     3421 AGCACTATGG AGATGACCGC CATGGCCACG GAGGACCCCC AGAGCGCCAC GGCCGGGACT 
     3481 CCCGTGATGG CTGGGGGGGC TACGGCTCCG ACAAGAGGCT GAGTGAAGGC CGGGGGCTGC 
     3541 CCCCTCCCCC CAGGGGTGGC CGTGACTGGG GAGAGCACAA CCAGCGGCTA GAGGAGCACC 
     3601 AGGCACGCGC CTGGCAGGGT GCCATGGACG CAGGCGCGGC TAGCCGGGAG CACGCCAGGT 
     3661 GGCAAGGTGG CGAGAGGGGC CTGTCTGGGC CCTCGGGGCC GGGGCACATG GCAAGCCGCG 
     3721 GTGGAGTGGC GGGGCGAGGC GGCTTTGCAC AAGGTGGACA TTCCCAGGGC CACGTGGTGC 
     3781 CAGGTGGCGG ACTGGAAGGT GGCGGAGTGG CCAGCCAGGA CCGGGGCAGC AGAGTCCCTC 
     3841 ACCCACACCC TCATCCCCCC CCGTACCCCC ACTTCACCCG CCGCTACACG CGTACGCGGC 
     3901 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3961 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     4021 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     4081 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     4141 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     4201 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     4261 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4321 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4381 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4441 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4501 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4561 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4621 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4681 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4741 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4801 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4861 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4921 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4981 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5041 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5101 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5161 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5221 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5281 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5341 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5401 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5461 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5521 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5581 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5641 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5701 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5761 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5821 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5881 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5941 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     6001 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     6061 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     6121 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     6181 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     6241 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6301 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6361 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6421 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6481 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6541 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6601 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6661 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6721 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6781 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6841 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6901 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6961 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     7021 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     7081 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     7141 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     7201 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     7261 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7321 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7381 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7441 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7501 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7561 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7621 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7681 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7741 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7801 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7861 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7921 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7981 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     8041 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     8101 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     8161 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     8221 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     8281 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8341 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8401 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8461 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8521 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8581 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8641 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8701 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8761 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8821 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8881 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8941 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     9001 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     9061 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     9121 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     9181 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     9241 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9301 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9361 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//