LOCUS       RG221139                8708 bp    DNA     circular  SYN 
DEFINITION  ADAM2 (tGFP-tagged) - Human ADAM metallopeptidase domain 2 (ADAM2)
ACCESSION   NM_001464
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3189..3893 
                     /label="GFP"  
     misc_feature    3940..4525 
                     /label="PolyA signal"  
     misc_feature    6617..7262 
                     /label="ColE1"  
     misc_feature    4979..5773 
                     /label="Neo"  
     misc_feature    4610..4912 
                     /label="SV40 ori"  
     misc_feature    7277..8137 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3168..3174 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3168 
                     /label="ADAM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCGCGTC TTGTTTCTGC TCAGCGGGCT CGGCGGGCTG CGGATGGACA 
     1081 GTAATTTTGA TAGTTTACCT GTGCAAATTA CAGTTCCGGA GAAAATACGG TCAATAATAA 
     1141 AGGAAGGAAT TGAATCGCAG GCATCCTACA AAATTGTAAT TGAAGGGAAA CCATATACTG 
     1201 TGAATTTAAT GCAAAAAAAC TTTTTACCCC ATAATTTTAG AGTTTACAGT TATAGTGGCA 
     1261 CAGGAATTAT GAAACCACTT GACCAAGATT TTCAGAATTT CTGCCACTAC CAAGGGTATA 
     1321 TTGAAGGTTA TCCAAAATCT GTGGTGATGG TTAGCACATG TACTGGACTC AGGGGCGTAC 
     1381 TACAGTTTGA AAATGTTAGT TATGGAATAG AACCCCTGGA GTCTTCAGTT GGCTTTGAAC 
     1441 ATGTAATTTA CCAAGTAAAA CATAAGAAAG CAGATGTTTC CTTATATAAT GAGAAGGATA 
     1501 TTGAATCAAG AGATCTGTCC TTTAAATTAC AAAGCGTAGA GTATAATCAT ATGGGGTCTG 
     1561 ATACAACTGT TGTCGCTCAA AAAGTTTTCC AGTTGATTGG ATTGACGAAT GCTATTTTTG 
     1621 TTTCATTTAA TATTACAATT ATTCTGTCTT CATTGGAGCT TTGGATAGAT GAAAATAAAA 
     1681 TTGCAACCAC TGGAGAAGCT AATGAGTTAT TACACACATT TTTAAGATGG AAAACATCTT 
     1741 ATCTTGTTTT ACGTCCTCAT GATGTGGCAT TTTTACTTGT TTACAGAGAA AAGTCAAATT 
     1801 ATGTTGGTGC AACCTTTCAA GGGAAGATGT GTGATGCAAA CTATGCAGGA GGTGTTGTTC 
     1861 TGCACCCCAG AACCATAAGT CTGGAATCAC TTGCAGTTAT TTTAGCTCAA TTATTGAGCC 
     1921 TTAGTATGGG GATCACTTAT GATGACATTA ACAAATGCCA GTGCTCAGGA GCTGTCTGCA 
     1981 TTATGAATCC AGAAGCAATT CATTTCAGTG GTGTGAAGAT CTTTAGTAAC TGCAGCTTCG 
     2041 AAGACTTTGC ACATTTTATT TCAAAGCAGA AGTCCCAGTG TCTTCACAAT CAGCCTCGCT 
     2101 TAGATCCTTT TTTCAAACAG CAAGCAGTGT GTGGTAATGC AAAGCTGGAA GCAGGAGAGG 
     2161 AGTGTGACTG TGGGACTGAA CAGGATTGTG CCCTTATTGG AGAAACATGC TGTGATATTG 
     2221 CCACATGTAG ATTTAAAGCC GGTTCAAACT GTGCTGAAGG ACCATGCTGC GAAAACTGTC 
     2281 TATTTATGTC AAAAGAAAGA ATGTGTAGGC CTTCCTTTGA AGAATGCGAC CTCCCTGAAT 
     2341 ATTGCAATGG ATCATCTGCA TCATGCCCAG AAAACCACTA TGTTCAGACT GGGCATCCGT 
     2401 GTGGACTGAA TCAATGGATC TGTATAGATG GAGTTTGTAT GAGTGGGGAT AAACAATGTA 
     2461 CAGACACATT TGGCAAAGAA GTAGAGTTTG GCCCTTCAGA ATGTTATTCT CACCTTAATT 
     2521 CAAAGACTGA TGTATCTGGA AACTGTGGTA TAAGTGATTC AGGATACACA CAGTGTGAAG 
     2581 CTGACAATCT GCAGTGCGGA AAATTAATAT GTAAATATGT AGGTAAATTT TTATTACAAA 
     2641 TTCCAAGAGC CACTATTATT TATGCCAACA TAAGTGGACA TCTCTGCATT GCTGTGGAAT 
     2701 TTGCCAGTGA TCATGCAGAC AGCCAAAAGA TGTGGATAAA AGATGGAACT TCTTGTGGTT 
     2761 CAAATAAGGT TTGCAGGAAT CAAAGATGTG TGAGTTCTTC ATACTTGGGT TATGATTGTA 
     2821 CTACTGACAA ATGCAATGAT AGAGGTGTAT GCAATAACAA AAAGCACTGT CACTGTAGTG 
     2881 CTTCATATTT ACCTCCAGAT TGCTCAGTTC AATCAGATCT ATGGCCTGGT GGGAGTATTG 
     2941 ACAGTGGCAA TTTTCCACCT GTAGCTATAC CAGCCAGACT CCCTGAAAGG CGCTACATTG 
     3001 AGAACATTTA CCATTCCAAA CCAATGAGAT GGCCATTTTT CTTATTCATT CCTTTCTTTA 
     3061 TTATTTTCTG TGTACTGATT GCTATAATGG TGAAAGTTAA TTTCCAAAGG AAAAAATGGA 
     3121 GAACTGAGGA CTATTCAAGC GATGAGCAAC CTGAAAGTGA GAGTGAACCT AAAGGGACGC 
     3181 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3241 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3301 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3361 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     3421 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     3481 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     3541 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     3601 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     3661 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     3721 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     3781 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     3841 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     3901 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3961 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4021 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4081 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4141 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4201 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4261 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4321 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4381 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4441 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4501 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4561 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     4621 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     4681 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     4741 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     4801 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     4861 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     4921 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     4981 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5041 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5101 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5161 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5221 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5281 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     5341 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     5401 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     5461 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     5521 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     5581 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     5641 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     5701 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     5761 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     5821 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     5881 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     5941 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6001 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6061 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6121 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6181 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6241 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6301 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6361 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     6421 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     6481 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     6541 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     6601 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     6661 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     6721 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     6781 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     6841 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     6901 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     6961 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7021 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7081 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7141 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7201 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7261 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7321 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7381 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     7441 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     7501 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     7561 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     7621 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     7681 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     7741 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     7801 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     7861 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     7921 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     7981 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8041 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8101 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8161 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8221 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8281 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8341 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8401 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     8461 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     8521 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     8581 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     8641 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     8701 CGAATTTT
//