LOCUS RG220700 9617 bp DNA circular SYN DEFINITION IDE (GFP-tagged) - Human insulin-degrading enzyme (IDE), transcript variant 1 ACCESSION NM_004969 VERSION NM_004969.1 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4098..4802 /label="GFP" misc_feature 4849..5434 /label="PolyA signal" misc_feature 7526..8171 /label="ColE1" misc_feature 5888..6682 /label="Neo" misc_feature 5519..5821 /label="SV40 ori" misc_feature 8186..9046 /label="Amp" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4077..4083 /label="Restriction Enzyme" misc_feature 1020..4077 /label="IDE" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GCGGTACCGG CTAGCGTGGC TTCTGCACCC CGCACTGCCC AGCACCTTCC 1081 GCTCAGTCCT CGGCGCCCGC CTGCCGCCTC CGGAGCGCCT GTGTGGTTTC CAAAAAAAGA 1141 CTTACAGCAA AATGAATAAT CCAGCCATCA AGAGAATAGG AAATCACATT ACCAAGTCTC 1201 CTGAAGACAA GCGAGAATAT CGAGGGCTAG AGCTGGCCAA TGGTATCAAA GTACTTCTTA 1261 TCAGTGATCC CACCACGGAT AAGTCATCAG CAGCACTTGA TGTGCACATA GGTTCATTGT 1321 CGGATCCTCC AAATATTGCT GGCTTAAGTC ATTTTTGTGA ACATATGCTT TTTTTGGGAA 1381 CAAAGAAATA CCCTAAAGAA AATGAATACA GCCAGTTTCT CAGTGAGCAT GCAGGAAGTT 1441 CAAATGCCTT TACTAGTGGA GAGCATACCA ATTACTATTT TGATGTTTCT CATGAACACC 1501 TAGAAGGTGC CCTAGACAGG TTTGCACAGT TTTTTCTGTG CCCCTTGTTC GATGAAAGTT 1561 GCAAAGACAG AGAGGTGAAT GCAGTTGATT CAGAACATGA GAAGAATGTG ATGAATGATG 1621 CCTGGAGACT CTTTCAATTG GAAAAAGCTA CAGGGAATCC TAAACACCCC TTCAGTAAAT 1681 TTGGGACAGG TAACAAATAT ACTCTGGAGA CTAGACCAAA CCAAGAAGGC ATTGATGTAA 1741 GACAAGAGCT ACTGAAATTC CATTCTGCTT ACTATTCATC CAACTTAATG GCTGTTTGTG 1801 TTTTAGGTCG AGAATCTTTA GATGACTTGA CTAATCTGGT GGTAAAGTTA TTTTCTGAAG 1861 TAGAGAACAA AAATGTTCCA TTGCCAGAAT TTCCTGAACA CCCTTTCCAA GAAGAACATC 1921 TTAAACAACT TTACAAAATA GTACCCATTA AAGATATTAG GAATCTCTAT GTGACATTTC 1981 CCATACCTGA CCTTCAGAAA TACTACAAAT CAAATCCTGG TCATTATCTT GGTCATCTCA 2041 TTGGGCATGA AGGTCCTGGA AGTCTGTTAT CAGAACTTAA GTCAAAGGGC TGGGTTAATA 2101 CTCTTGTTGG TGGGCAGAAG GAAGGAGCCC GAGGTTTTAT GTTTTTTATC ATTAATGTGG 2161 ACTTGACCGA GGAAGGATTA TTACATGTTG AAGATATAAT TTTGCACATG TTTCAATACA 2221 TTCAGAAGTT ACGTGCAGAA GGACCTCAAG AATGGGTTTT CCAAGAGTGC AAGGACTTGA 2281 ATGCTGTTGC TTTTAGGTTT AAAGACAAAG AGAGGCCACG GGGCTATACA TCTAAGATTG 2341 CAGGAATATT GCATTATTAT CCCCTAGAAG AGGTGCTCAC AGCGGAATAT TTACTGGAAG 2401 AATTTAGACC TGACTTAATA GAGATGGTTC TCGATAAACT CAGACCAGAA AATGTCCGGG 2461 TTGCCATAGT TTCTAAATCT TTTGAAGGAA AAACTGATCG CACAGAAGAG TGGTATGGAA 2521 CCCAGTACAA ACAAGAAGCT ATACCGGATG AAGTCATCAA GAAATGGCAA AATGCTGACC 2581 TGAATGGGAA ATTTAAACTT CCTACAAAGA ATGAATTTAT TCCTACGAAT TTTGAGATTT 2641 TACCGTTAGA AAAAGAGGCG ACACCATACC CTGCTCTTAT TAAGGATACA GCTATGAGCA 2701 AACTTTGGTT CAAACAAGAT GATAAGTTTT TTTTGCCGAA GGCTTGTCTC AACTTTGAAT 2761 TTTTCAGCCC ATTTGCTTAT GTGGACCCCT TGCACTGTAA CATGGCCTAT TTGTACCTTG 2821 AGCTCCTCAA AGACTCACTC AACGAGTATG CATATGCAGC AGAGCTAGCA GGCTTGAGCT 2881 ATGATCTCCA AAATACCATC TATGGGATGT ATCTTTCAGT GAAAGGTTAC AATGACAAGC 2941 AGCCAATTTT ACTAAAGAAG ATTATTGAGA AAATGGCTAC CTTTGAGATT GATGAAAAAA 3001 GATTTGAAAT TATCAAAGAA GCATATATGC GATCTCTTAA CAATTTCCGG GCTGAACAGC 3061 CTCACCAGCA TGCCATGTAC TACCTCCGCT TGCTGATGAC TGAAGTGGCC TGGACTAAAG 3121 ATGAGTTAAA AGAAGCTCTG GATGATGTAA CCCTTCCTCG CCTTAAGGCC TTCATACCTC 3181 AGCTCCTGTC ACGGCTGCAC ATTGAAGCCC TTCTCCATGG AAACATAACA AAGCAGGCTG 3241 CATTAGGAAT TATGCAGATG GTTGAAGACA CCCTCATTGA ACATGCTCAT ACCAAACCTC 3301 TCCTTCCAAG TCAGCTGGTT CGGTATAGAG AAGTTCAGCT CCCTGACAGA GGATGGTTTG 3361 TTTATCAGCA GAGAAATGAA GTTCACAATA ACTGTGGCAT CGAGATATAC TACCAAACAG 3421 ACATGCAAAG CACCTCAGAG AATATGTTTC TGGAGCTCTT CTGTCAGATT ATCTCGGAAC 3481 CTTGCTTCAA CACCCTGCGC ACCAAGGAGC AGTTGGGCTA TATCGTCTTC AGCGGGCCAC 3541 GTCGAGCTAA TGGCATACAG GGCTTGAGAT TCATCATCCA GTCAGAAAAG CCACCTCACT 3601 ACCTAGAAAG CAGAGTGGAA GCTTTCTTAA TTACCATGGA AAAGTCCATA GAGGACATGA 3661 CAGAAGAGGC CTTCCAAAAA CACATTCAGG CATTAGCAAT TCGTCGACTA GACAAACCAA 3721 AGAAGCTATC TGCTGAGTGT GCTAAATACT GGGGAGAAAT CATCTCCCAG CAATATAATT 3781 TTGACAGAGA TAACACTGAG GTTGCATATT TAAAGACACT TACCAAGGAA GATATCATCA 3841 AATTCTACAA GGAAATGTTG GCAGTAGATG CTCCAAGGAG ACATAAGGTA TCCGTCCATG 3901 TTCTTGCCAG GGAAATGGAT TCTTGTCCTG TTGTTGGAGA GTTCCCATGT CAAAATGACA 3961 TAAATTTGTC ACAAGCACCA GCCTTGCCAC AACCTGAAGT GATTCAGAAC ATGACCGAAT 4021 TCAAGCGTGG TCTGCCACTG TTTCCCCTTG TGAAACCACA TATTAACTTC ATGGCTGCAA 4081 AACTCACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 4141 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 4201 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 4261 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 4321 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 4381 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 4441 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 4501 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 4561 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 4621 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 4681 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 4741 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 4801 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 4861 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 4921 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 4981 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 5041 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 5101 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 5161 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 5221 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 5281 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 5341 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 5401 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 5461 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 5521 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 5581 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 5641 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 5701 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 5761 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 5821 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 5881 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 5941 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 6001 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 6061 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 6121 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 6181 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 6241 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 6301 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 6361 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 6421 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 6481 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 6541 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 6601 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 6661 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 6721 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 6781 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 6841 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 6901 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 6961 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 7021 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 7081 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 7141 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 7201 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 7261 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 7321 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 7381 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 7441 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 7501 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 7561 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 7621 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 7681 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 7741 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 7801 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 7861 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 7921 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 7981 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 8041 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 8101 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 8161 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 8221 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 8281 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 8341 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 8401 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 8461 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 8521 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 8581 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 8641 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 8701 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 8761 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 8821 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 8881 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 8941 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 9001 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 9061 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 9121 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 9181 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 9241 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 9301 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 9361 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 9421 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 9481 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 9541 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 9601 AATTTAACGC GAATTTT //