LOCUS       RG220635                9080 bp    DNA     circular  SYN 
DEFINITION  HSPA4 (tGFP-tagged) - Human heat shock 70kDa protein 4 (HSPA4)
ACCESSION   NM_002154
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3561..4265 
                     /label="GFP"  
     misc_feature    4312..4897 
                     /label="PolyA signal"  
     misc_feature    6989..7634 
                     /label="ColE1"  
     misc_feature    5351..6145 
                     /label="Neo"  
     misc_feature    4982..5284 
                     /label="SV40 ori"  
     misc_feature    7649..8509 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3540..3546 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3540 
                     /label="HSPA4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGTGGTG GGCATAGACC TGGGCTTCCA GAGCTGCTAC GTCGCTGTGG 
     1081 CCCGCGCCGG CGGCATCGAG ACTATCGCTA ATGAGTATAG CGACCGCTGC ACGCCGGCTT 
     1141 GCATTTCTTT TGGTCCTAAG AATCGTTCAA TTGGAGCAGC AGCTAAAAGC CAGGTAATTT 
     1201 CTAATGCAAA GAACACAGTC CAAGGATTTA AAAGATTCCA TGGCCGAGCA TTCTCTGATC 
     1261 CATTTGTGGA GGCAGAAAAA TCTAACCTTG CATATGATAT TGTGCAGTTG CCTACAGGAT 
     1321 TAACAGGTAT AAAGGTGACA TATATGGAGG AAGAGCGAAA TTTTACCACT GAGCAAGTGA 
     1381 CTGCCATGCT TTTGTCCAAA CTGAAGGAGA CAGCCGAAAG TGTTCTTAAG AAGCCTGTAG 
     1441 TTGACTGTGT TGTTTCGGTT CCTTGTTTCT ATACTGATGC AGAAAGACGA TCAGTGATGG 
     1501 ATACAACACA GATTGCTGGT CTTAATTGCT TGCGATTAAT GAATGAAACC ACTGCAGTTG 
     1561 CTCTTGCATA TGGAATCTAT AAGCAGGATC TTCCTGCCTT AGAAGAGAAA CCAAGAAATG 
     1621 TAGTTTTTGT AGACATGGGC CACTCTGCTT ATCAAGTTTC TGTATGTGCA TTTAATAGAG 
     1681 GAAAACTGAA AGTTCTGGCC ACTGCATTTG ACACGACATT GGGAGGTAGA AAATTTGATG 
     1741 AAGTGTTAGT AAATCACTTC TGTGAAGAAT TTGGGAAGAA ATACAAGCTA GACATTAAGT 
     1801 CCAAAATCCG TGCATTATTA CGACTCTCTC AGGAGTGTGA GAAACTCAAG AAATTGATGA 
     1861 GTGCAAATGC TTCAGATCTC CCTTTGAGCA TTGAATGTTT TATGAATGAT GTTGATGTAT 
     1921 CTGGAACTAT GAATAGAGGC AAATTTCTGG AGATGTGCAA TGATCTCTTA GCTAGAGTGG 
     1981 AGCCACCACT TCGTAGTGTT TTGGAACAAA CCAAGTTAAA GAAAGAAGAT ATTTATGCAG 
     2041 TGGAGATAGT TGGTGGTGCT ACACGAATCC CTGCGGTAAA AGAGAAGATC AGCAAATTTT 
     2101 TCGGTAAAGA ACTTAGTACA ACATTAAATG CTGATGAAGC TGTCACTCGA GGCTGTGCAT 
     2161 TGCAGTGTGC CATCTTATCG CCTGCTTTCA AAGTCAGAGA ATTTTCTATC ACTGATGTAG 
     2221 TACCATATCC AATATCTCTG AGATGGAATT CTCCAGCTGA AGAAGGGTCA AGTGACTGTG 
     2281 AAGTCTTTTC CAAAAATCAT GCTGCTCCTT TCTCTAAAGT TCTTACATTT TATAGAAAGG 
     2341 AACCTTTCAC TCTTGAGGCC TACTACAGCT CTCCTCAGGA TTTGCCCTAT CCAGATCCTG 
     2401 CTATAGCTCA GTTTTCAGTT CAGAAAGTCA CTCCTCAGTC TGATGGCTCC AGTTCAAAAG 
     2461 TGAAAGTCAA AGTTCGAGTA AATGTCCATG GCATTTTCAG TGTGTCCAGT GCATCTTTAG 
     2521 TGGAGGTTCA CAAGTCTGAG GAAAATGAGG AGCCAATGGA AACAGATCAG AATGCAAAGG 
     2581 AGGAAGAGAA GATGCAAGTG GACCAGGAGG AACCACATGT TGAAGAGCAA CAGCAGCAGA 
     2641 CACCAGCAGA AAATAAGGCA GAGTCTGAAG AAATGGAGAC CTCTCAAGCT GGATCCAAGG 
     2701 ATAAAAAGAT GGACCAACCA CCCCAAGCCA AGAAGGCAAA AGTGAAGACC AGTACTGTGG 
     2761 ACCTGCCAAT CGAGAATCAG CTATTATGGC AGATAGACAG AGAGATGCTC AACTTGTACA 
     2821 TTGAAAATGA GGGTAAGATG ATCATGCAGG ATAAACTGGA GAAGGAGCGG AATGATGCTA 
     2881 AGAACGCAGT GGAGGAATAT GTGTATGAAA TGAGAGACAA GCTTAGTGGT GAATATGAGA 
     2941 AGTTTGTGAG TGAAGATGAT CGTAACAGTT TTACTTTGAA ACTGGAAGAT ACTGAAAATT 
     3001 GGTTGTATGA GGATGGAGAA GACCAGCCAA AGCAAGTTTA TGTTGATAAG TTGGCTGAAT 
     3061 TAAAAAATCT AGGTCAACCT ATTAAGATAC GTTTCCAGGA ATCTGAAGAA CGACCAAAAT 
     3121 TATTTGAAGA ACTAGGGAAA CAGATCCAAC AGTATATGAA AATAATCAGC TCTTTCAAAA 
     3181 ACAAGGAGGA CCAGTATGAT CATTTGGATG CTGCTGACAT GACAAAGGTA GAAAAAAGCA 
     3241 CAAATGAAGC AATGGAGTGG ATGAATAACA AGCTAAATCT GCAGAACAAG CAGAGTTTGA 
     3301 CCATGGATCC AGTTGTCAAG TCAAAAGAGA TTGAAGCTAA AATTAAGGAG CTGACAAGTA 
     3361 CTTGTAGCCC TATAATTTCA AAGCCCAAAC CCAAAGTGGA ACCTCCAAAA GAGGAACAAA 
     3421 AAAATGCAGA GCAGAATGGA CCAGTGGATG GACAAGGAGA CAACCCAGGC CCCCAGGCTG 
     3481 CTGAGCAGGG TACAGACACA GCTGTGCCTT CGGATTCAGA CAAGAAGCTT CCTGAAATGG 
     3541 ACATTGATAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3601 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3661 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     3721 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     3781 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     3841 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     3901 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     3961 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4021 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4081 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4141 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4201 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4261 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4321 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4381 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4441 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4501 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4561 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4621 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4681 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4741 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4801 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4861 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4921 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     4981 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5041 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5101 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5161 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5221 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5281 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5341 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5401 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5461 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5521 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5581 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5641 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     5701 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     5761 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     5821 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     5881 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     5941 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6001 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6061 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6121 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6181 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6241 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6301 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6361 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6421 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6481 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6541 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6601 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6661 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     6721 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     6781 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     6841 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     6901 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     6961 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7021 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7081 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7141 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7201 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7261 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7321 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7381 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7441 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7501 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7561 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7621 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7681 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     7741 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     7801 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     7861 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     7921 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     7981 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8041 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8101 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8161 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8221 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8281 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8341 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8401 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8461 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8521 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8581 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8641 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     8701 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     8761 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     8821 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     8881 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     8941 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9001 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9061 AAAAATTTAA CGCGAATTTT 
//