LOCUS       RG220621                8348 bp    DNA     circular  SYN 
DEFINITION  EEFSEC (tGFP-tagged) - Human eukaryotic elongation factor, selenocysteine-tRNA-specific (EEFSEC)
ACCESSION   NM_021937
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2829..3533 
                     /label="GFP"  
     misc_feature    3580..4165 
                     /label="PolyA signal"  
     misc_feature    6257..6902 
                     /label="ColE1"  
     misc_feature    4619..5413 
                     /label="Neo"  
     misc_feature    4250..4552 
                     /label="SV40 ori"  
     misc_feature    6917..7777 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2808..2814 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2808 
                     /label="EEFSEC"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGGGCGG CGGGTGAACG TGAACGTGGG CGTGCTGGGC CACATCGACA 
     1081 GCGGCAAGAC GGCGCTGGCG CGGGCGCTAA GCACCACAGC CTCCACCGCC GCCTTTGACA 
     1141 AGCAGCCGCA GAGCCGCGAG CGCGGCATCA CGCTCGATCT GGGCTTCTCG TGCTTCTCGG 
     1201 TGCCGCTGCC CGCGCGCCTG CGGTCGTCTT TGCCCGAGTT CCAGGCAGCG CCCGAGGCCG 
     1261 AGCCCGAGCC CGGCGAGCCA CTGCTTCAGG TCACGCTGGT CGACTGCCCC GGGCACGCCT 
     1321 CCCTCATCCG GACCATCATC GGCGGGGCCC AGATCATTGA TCTGATGATG CTGGTCATCG 
     1381 ATGTGACCAA GGGGATGCAG ACCCAGTCAG CGGAATGCCT TGTGATCGGC CAGATTGCCT 
     1441 GCCAGAAGCT GGTCGTGGTG CTGAACAAAA TAGACCTCTT ACCTGAAGGA AAGAGACAGG 
     1501 CAGCAATTGA TAAAATGACC AAGAAAATGC AGAAGACCCT AGAGAACACC AAGTTCCGAG 
     1561 GTGCACCGAT TATACCCGTG GCGGCCAAGC CGGGGGGACC AGAGGCCCCC GAAACTGAAG 
     1621 CTCCACAGGG CATTCCAGAG CTCATTGAGC TCCTGACGTC CCAGATTTCC ATCCCAACGA 
     1681 GAGACCCCTC GGGACCGTTC CTCATGTCTG TGGACCACTG TTTCTCCATC AAAGGCCAAG 
     1741 GCACTGTGAT GACAGGGACC ATCCTTTCAG GCTCCATCAG CCTCGGTGAC AGTGTGGAGA 
     1801 TCCCTGCCCT CAAGGTGGTG AAGAAGGTGA AGTCCATGCA GATGTTCCAC ATGCCCATCA 
     1861 CTTCAGCCAT GCAAGGAGAC CGGCTGGGCA TCTGCGTCAC CCAGTTTGAC CCTAAGCTGC 
     1921 TGGAGCGCGG GTTGGTGTGT GCCCCCGAGT CCCTGCACAC TGTCCATGCG GCCCTCATCT 
     1981 CTGTGGAAAA GATACCGTAT TTCCGGGGGC CCCTGCAAAC CAAGGCCAAG TTCCACATTA 
     2041 CAGTGGGCCA TGAAACAGTC ATGGGCCGGT TGATGTTCTT CAGTCCTGCT CCAGATAACT 
     2101 TTGACCAGGA GCCTATACTG GACTCTTTCA ACTTCTCTCA AGAATACCTT TTCCAGGAGC 
     2161 AGTACCTGTC CAAGGATTTG ACACCAGCAG TGACAGACAA TGATGAGGCC GACAAGAAGG 
     2221 CCGGCCAGGC CACAGAGGGC CATTGTCCTC GGCAGCAGTG GGCCCTGGTG GAGTTTGAGA 
     2281 AGCCCGTCAC CTGCCCTCGG CTGTGCCTGG TGATTGGCTC CAGGCTAGAT GCGGACATTC 
     2341 ACACCAACAC GTGCCGGCTA GCCTTCCATG GCATCCTGCT CCACGGGCTA GAGGACAGGA 
     2401 ACTACGCCGA CAGCTTCCTG CCCAGGCTGA AGGTGTACAA GCTGAAGCAC AAGCATGGCC 
     2461 TTGTGGAGCG GGCGATGGAT GACTACAGTG TGATCGGCCG CTCCCTGTTC AAAAAGGAAA 
     2521 CCAACATCCA GCTCTTCGTG GGGCTCAAGG TGCACTTGTC CACTGGGGAA CTGGGCATCA 
     2581 TCGACAGTGC CTTCGGCCAG AGCGGCAAGT TCAAGATCCA CATCCCAGGT GGCCTCAGCC 
     2641 CCGAGTCCAA GAAGATCCTG ACACCCGCCC TCAAGAAGCG GGCCCGGGCT GGCCGTGGGG 
     2701 AGGCCACCAG GCAGGAGGAG AGCGCCGAGC GGAGCGAGCC CTCACAGCAT GTGGTGCTCA 
     2761 GCCTGACTTT CAAGCGTTAT GTCTTCGACA CCCACAAGCG CATGGTTCAG TCTCCCACGC 
     2821 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     2881 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     2941 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3001 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     3061 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     3121 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     3181 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     3241 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     3301 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     3361 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     3421 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     3481 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     3541 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3601 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3661 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3721 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3781 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3841 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3901 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3961 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4021 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4081 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4141 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4201 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     4261 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     4321 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     4381 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     4441 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     4501 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     4561 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     4621 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     4681 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     4741 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     4801 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     4861 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     4921 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     4981 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     5041 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     5101 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     5161 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     5221 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     5281 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     5341 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     5401 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     5461 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     5521 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     5581 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     5641 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     5701 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     5761 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     5821 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     5881 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     5941 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6001 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     6061 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     6121 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     6181 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     6241 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     6301 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     6361 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     6421 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     6481 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     6541 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     6601 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     6661 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     6721 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     6781 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     6841 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     6901 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     6961 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7021 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     7081 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     7141 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     7201 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     7261 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     7321 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     7381 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     7441 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     7501 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     7561 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     7621 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     7681 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     7741 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     7801 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     7861 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     7921 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     7981 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8041 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     8101 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     8161 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     8221 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     8281 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     8341 CGAATTTT
//