LOCUS       RG220499                8852 bp    DNA     circular  SYN 
DEFINITION  TSHR (tGFP-tagged) - Human thyroid stimulating hormone receptor (TSHR), transcript variant 1
ACCESSION   NM_000369
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3333..4037 
                     /label="GFP"  
     misc_feature    4084..4669 
                     /label="PolyA signal"  
     misc_feature    6761..7406 
                     /label="ColE1"  
     misc_feature    5123..5917 
                     /label="Neo"  
     misc_feature    4754..5056 
                     /label="SV40 ori"  
     misc_feature    7421..8281 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3312..3318 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3312 
                     /label="TSHR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCCGGCG GACTTGCTGC AGCTGGTGCT GCTGCTCGAC CTGCCCAGGG 
     1081 ACCTGGGCGG AATGGGGTGT TCGTCTCCAC CCTGCGAGTG CCATCAGGAG GAGGACTTCA 
     1141 GAGTCACCTG CAAGGATATT CAACGCATCC CCAGCTTACC GCCCAGTACG CAGACTCTGA 
     1201 AGCTTATTGA GACTCACCTG AGAACTATTC CAAGTCATGC ATTTTCTAAT CTGCCCAATA 
     1261 TTTCCAGAAT CTACGTATCT ATAGATGTGA CTCTGCAGCA GCTGGAATCA CACTCCTTCT 
     1321 ACAATTTGAG TAAAGTGACT CACATAGAAA TTCGGAATAC CAGGAACTTA ACTTACATAG 
     1381 ACCCTGATGC CCTCAAAGAG CTCCCCCTCC TAAAGTTCCT TGGCATTTTC AACACTGGAC 
     1441 TTAAAATGTT CCCTGACCTG ACCAAAGTTT ATTCCACTGA TATATTCTTT ATACTTGAAA 
     1501 TTACAGACAA CCCTTACATG ACGTCAATCC CTGTGAATGC TTTTCAGGGA CTATGCAATG 
     1561 AAACCTTGAC ACTGAAGCTG TACAACAATG GCTTTACTTC AGTCCAAGGA TATGCTTTCA 
     1621 ATGGGACAAA GCTGGATGCT GTTTACCTAA ACAAGAATAA ATACCTGACA GTTATTGACA 
     1681 AAGATGCATT TGGAGGAGTA TACAGTGGAC CAAGCTTGCT GGACGTGTCT CAAACCAGTG 
     1741 TCACTGCCCT TCCATCCAAA GGCCTGGAGC ACCTGAAGGA ACTGATAGCA AGAAACACCT 
     1801 GGACTCTTAA GAAACTTCCA CTTTCCTTGA GTTTCCTTCA CCTCACACGG GCTGACCTTT 
     1861 CTTACCCAAG CCACTGCTGT GCTTTTAAGA ATCAGAAGAA AATCAGAGGA ATCCTTGAGT 
     1921 CCTTGATGTG TAATGAGAGC AGTATGCAGA GCTTGCGCCA GAGAAAATCT GTGAATGCCT 
     1981 TGAATAGCCC CCTCCACCAG GAATATGAAG AGAATCTGGG TGACAGCATT GTTGGGTACA 
     2041 AGGAAAAGTC CAAGTTCCAG GATACTCATA ACAACGCTCA TTATTACGTC TTCTTTGAAG 
     2101 AACAAGAGGA TGAGATCATT GGTTTTGGCC AGGAGCTCAA AAACCCCCAG GAAGAGACTC 
     2161 TACAAGCTTT TGACAGCCAT TATGACTACA CCATATGTGG GGACAGTGAA GACATGGTGT 
     2221 GTACCCCCAA GTCCGATGAG TTCAACCCGT GTGAAGACAT AATGGGCTAC AAGTTCCTGA 
     2281 GAATTGTGGT GTGGTTCGTT AGTCTGCTGG CTCTCCTGGG CAATGTCTTT GTCCTGCTTA 
     2341 TTCTCCTCAC CAGCCACTAC AAACTGAACG TCCCCCGCTT TCTCATGTGC AACCTGGCCT 
     2401 TTGCGGATTT CTGCATGGGG ATGTACCTGC TCCTCATCGC CTCTGTAGAC CTCTACACTC 
     2461 ACTCTGAGTA CTACAACCAT GCCATCGACT GGCAGACAGG CCCTGGGTGC AACACGGCTG 
     2521 GTTTCTTCAC TGTCTTTGCA AGCGAGTTAT CGGTGTATAC GCTGACGGTC ATCACCCTGG 
     2581 AGCGCTGGTA TGCCATCACC TTCGCCATGC GCCTGGACCG GAAGATCCGC CTCAGGCACG 
     2641 CATGTGCCAT CATGGTTGGG GGCTGGGTTT GCTGCTTCCT TCTCGCCCTG CTTCCTTTGG 
     2701 TGGGAATAAG TAGCTATGCC AAAGTCAGTA TCTGCCTGCC CATGGACACC GAGACCCCTC 
     2761 TTGCTCTGGC ATATATTGTT TTTGTTCTGA CGCTCAACAT AGTTGCCTTC GTCATCGTCT 
     2821 GCTGCTGTTA TGTGAAGATC TACATCACAG TCCGAAATCC GCAGTACAAC CCAGGGGACA 
     2881 AAGATACCAA AATTGCCAAG AGGATGGCTG TGTTGATCTT CACCGACTTC ATATGCATGG 
     2941 CCCCAATCTC ATTCTATGCT CTGTCAGCAA TTCTGAACAA GCCTCTCATC ACTGTTAGCA 
     3001 ACTCCAAAAT CTTGCTGGTA CTCTTCTATC CACTTAACTC CTGTGCCAAT CCATTCCTCT 
     3061 ATGCTATTTT CACCAAGGCC TTCCAGAGGG ATGTGTTCAT CCTACTCAGC AAGTTTGGCA 
     3121 TCTGTAAACG CCAGGCTCAG GCATACCGGG GGCAGAGGGT TCCTCCAAAG AACAGCACTG 
     3181 ATATTCAGGT TCAAAAGGTT ACCCACGAGA TGAGGCAGGG TCTCCACAAC ATGGAAGATG 
     3241 TCTATGAACT GATTGAAAAC TCCCATCTAA CCCCAAAGAA GCAAGGCCAA ATCTCAGAAG 
     3301 AGTATATGCA AACGGTTTTG ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3361 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3421 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3481 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3541 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3601 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     3661 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     3721 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     3781 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     3841 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     3901 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     3961 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4021 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4081 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4141 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4201 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4261 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4321 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4381 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4441 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4501 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4561 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4621 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4681 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     4741 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     4801 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     4861 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     4921 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     4981 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5041 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5101 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5161 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5221 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5281 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5341 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5401 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5461 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5521 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5581 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     5641 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     5701 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     5761 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     5821 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     5881 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     5941 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6001 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6061 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6121 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6181 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6241 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6301 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6361 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6421 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6481 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6541 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6601 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     6661 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     6721 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     6781 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     6841 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     6901 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     6961 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7021 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7081 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7141 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7201 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7261 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7321 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7381 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7441 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7501 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7561 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7621 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     7681 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     7741 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     7801 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     7861 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     7921 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     7981 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8041 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8101 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8161 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8221 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8281 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8341 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8401 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8461 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8521 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8581 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     8641 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     8701 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     8761 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     8821 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//