LOCUS       RG220326                8177 bp    DNA     circular  SYN 
DEFINITION  EPHA3 (tGFP-tagged) - Human EPH receptor A3 (EPHA3), transcript variant 2
ACCESSION   NM_182644
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2658..3362 
                     /label="GFP"  
     misc_feature    3409..3994 
                     /label="PolyA signal"  
     misc_feature    6086..6731 
                     /label="ColE1"  
     misc_feature    4448..5242 
                     /label="Neo"  
     misc_feature    4079..4381 
                     /label="SV40 ori"  
     misc_feature    6746..7606 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2637..2643 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2637 
                     /label="EPHA3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTGTCAG CTCTCCATCC TCCTCCTTCT CAGCTGCTCT GTTCTCGACA 
     1081 GCTTCGGGGA ACTGATTCCG CAGCCTTCCA ATGAAGTCAA TCTACTGGAT TCAAAAACAA 
     1141 TTCAAGGGGA GCTGGGCTGG ATCTCTTATC CATCACATGG GTGGGAAGAG ATCAGTGGTG 
     1201 TGGATGAACA TTACACACCC ATCAGGACTT ACCAGGTGTG CAATGTCATG GACCACAGTC 
     1261 AAAACAATTG GCTGAGAACA AACTGGGTCC CCAGGAACTC AGCTCAGAAG ATTTATGTGG 
     1321 AGCTCAAGTT CACTCTACGA GACTGCAATA GCATTCCATT GGTTTTAGGA ACTTGCAAGG 
     1381 AGACATTCAA CCTGTACTAC ATGGAGTCTG ATGATGATCA TGGGGTGAAA TTTCGAGAGC 
     1441 ATCAGTTTAC AAAGATTGAC ACCATTGCAG CTGATGAAAG TTTCACTCAA ATGGATCTTG 
     1501 GGGACCGTAT TCTGAAGCTC AACACTGAGA TTAGAGAAGT AGGTCCTGTC AACAAGAAGG 
     1561 GATTTTATTT GGCATTTCAA GATGTTGGTG CTTGTGTTGC CTTGGTGTCT GTGAGAGTAT 
     1621 ACTTCAAAAA GTGCCCATTT ACAGTGAAGA ATCTGGCTAT GTTTCCAGAC ACGGTACCCA 
     1681 TGGACTCCCA GTCCCTGGTG GAGGTTAGAG GGTCTTGTGT CAACAATTCT AAGGAGGAAG 
     1741 ATCCTCCAAG GATGTACTGC AGTACAGAAG GCGAATGGCT TGTACCCATT GGCAAGTGTT 
     1801 CCTGCAATGC TGGCTATGAA GAAAGAGGTT TTATGTGCCA AGCTTGTCGA CCAGGTTTCT 
     1861 ACAAGGCATT GGATGGTAAT ATGAAGTGTG CTAAGTGCCC GCCTCACAGT TCTACTCAGG 
     1921 AAGATGGTTC AATGAACTGC AGGTGTGAGA ATAATTACTT CCGGGCAGAC AAAGACCCTC 
     1981 CATCCATGGC TTGTACCCGA CCTCCATCTT CACCAAGAAA TGTTATCTCT AATATAAACG 
     2041 AGACCTCAGT TATCCTGGAC TGGAGTTGGC CCCTGGACAC AGGAGGCCGG AAAGATGTTA 
     2101 CCTTCAACAT CATATGTAAA AAATGTGGGT GGAATATAAA ACAGTGTGAG CCATGCAGCC 
     2161 CAAATGTCCG CTTCCTCCCT CGACAGTTTG GACTCACCAA CACCACGGTG ACAGTGACAG 
     2221 ACCTTCTGGC ACATACTAAC TACACCTTTG AGATTGATGC CGTTAATGGG GTGTCAGAGC 
     2281 TGAGCTCCCC ACCAAGACAG TTTGCTGCGG TCAGCATCAC AACTAATCAG GCTGCTCCAT 
     2341 CACCTGTCCT GACGATTAAG AAAGATCGGA CCTCCAGAAA TAGCATCTCT TTGTCCTGGC 
     2401 AAGAACCTGA ACATCCTAAT GGGATCATAT TGGACTACGA GGTCAAATAC TATGAAAAGC 
     2461 AGGAACAAGA AACAAGTTAT ACCATTCTGA GGGCAAGAGG CACAAATGTT ACCATCAGTA 
     2521 GCCTCAAGCC TGACACTATA TACGTATTCC AAATCCGAGC CCGAACAGCC GCTGGATATG 
     2581 GGACGAACAG CCGCAAGTTT GAGTTTGAAA CTAGTCCAGA CTGTATGTAT TATTTCAATG 
     2641 CAGTCACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     2701 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     2761 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     2821 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     2881 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     2941 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     3001 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     3061 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     3121 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     3181 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     3241 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     3301 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     3361 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3421 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3481 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3541 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3601 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3661 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3721 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3781 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3841 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3901 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3961 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4021 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     4081 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     4141 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4201 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     4261 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     4321 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     4381 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     4441 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     4501 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     4561 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     4621 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     4681 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     4741 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     4801 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     4861 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     4921 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     4981 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     5041 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     5101 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     5161 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     5221 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     5281 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     5341 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     5401 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     5461 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     5521 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     5581 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     5641 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     5701 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     5761 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     5821 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     5881 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     5941 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     6001 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     6061 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     6121 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     6181 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     6241 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     6301 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     6361 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     6421 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     6481 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     6541 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     6601 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     6661 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     6721 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     6781 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     6841 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     6901 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     6961 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     7021 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     7081 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     7141 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     7201 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     7261 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     7321 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     7381 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     7441 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     7501 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     7561 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     7621 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     7681 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     7741 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     7801 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     7861 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     7921 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     7981 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     8041 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     8101 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     8161 AATTTAACGC GAATTTT
//