LOCUS       RG220170                9269 bp    DNA     circular  SYN 
DEFINITION  MRVI1 (tGFP-tagged) - Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 1
ACCESSION   NM_001098579
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3750..4454 
                     /label="GFP"  
     misc_feature    4501..5086 
                     /label="PolyA signal"  
     misc_feature    7178..7823 
                     /label="ColE1"  
     misc_feature    5540..6334 
                     /label="Neo"  
     misc_feature    5171..5473 
                     /label="SV40 ori"  
     misc_feature    7838..8698 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3729..3735 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3729 
                     /label="IRAG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAATGGAT TTGACTTGTC CTTTTGGCGT CTCCCCAGCC TGTGGAGCCC 
     1081 AGGCCTCTTG GAGCATCTTT GGGGCTGACG CAGCGGAGGT TCCGGGCACA CGTGGCCACT 
     1141 CCCAGCAGGA GGCTGCCATG CCCCACATTC CCGAGGACGA GGAGCCCCCC GGAGAGCCAC 
     1201 AGGCAGCCCA GAGCCCTGCC GGCCAAGGTC CTCCTACCGC AGGAGTATCT TGCAGTCCAA 
     1261 CTCCCACGAT TGTCCTGACT GGGGATGCCA CTTCACCAGA AGGAGAAACC GACAAAAACC 
     1321 TGGCCAACAG AGTTCACAGT CCCCACAAGA GGCTTTCTCA CCGACACTTG AAGGTGTCCA 
     1381 CTGCCTCCCT GACATCTGTG GACCCCGCGG GGCACATCAT TGACCTGGTG AATGACCAGC 
     1441 TGCCAGACAT CAGCATCTCA GAGGAGGACA AGAAGAAAAA CCTGGCGCTG CTGGAAGAAG 
     1501 CCAAGTTGGT GAGTGAGCGA TTCCTGACCC GCCGTGGGAG GAAGTCCAGG AGCAGCCCCG 
     1561 GAGACTCCCC ATCAGCTGTT TCCCCGAACC TCAGCCCCAG CGCTTCTCCT ACATCCTCTC 
     1621 GGAGCAACTC ACTTACAGTC CCCACCCCGC CAGGTTTGGA TGTGTGCAGT GGCCCGCCAT 
     1681 CCCCTCTGCC TGGAGCACCA CCACAGAAGG GGGATGAGGC CGACGTCTCT TCACCTCACC 
     1741 CTGGCGAGCC TAACGTCCCC AAAGGGCTAG CTGACAGGAA GCAGAATGAC CAGAGGAAAG 
     1801 TGTCTCAGGG CAGGCTGGCT CCTCGTCCTC CTCCAGTTGA GAAGTCCAAA GAGATTGCAA 
     1861 TAGAACAAAA GGAAAACTTC GATCCCCTCC AGTACCCCGA GACCACACCC AAAGGCCTAG 
     1921 CTCCTGTTAC AAACAGCAGT GGGAAAATGG CCCTGAACAG CCCTCAGCCT GGCCCCGTGG 
     1981 AGAGCGAGCT GGGGAAGCAG CTCTTGAAAA CGGGCTGGGA GGGCAGCCCT CTGCCGAGAA 
     2041 GTCCAACCCA GGATGCGGCA GGAGTGGGTC CCCCAGCCTC CCAGGGGAGA GGCCCAGCTG 
     2101 GAGAGCCGAT GGGGCCCGAG GCTGGCTCCA AAGCTGAGCT TCCACCCACT GTGTCCCGGC 
     2161 CCCCGCTGCT GCGAGGGCTC TCCTGGGACA GTGGCCCTGA AGAACCTGGC CCCCGGCTGC 
     2221 AGAAAGTGCT TGCCAAGCTG CCACTGGCAG AGGAAGAAAA GCGTTTTGCA GGCAAGGCCG 
     2281 GCGGCAAGCT GGCCAAGGCC CCTGGTCTCA AAGACTTTCA GATACAAGTG CAGCCCGTGC 
     2341 GGATGCAGAA ACTGACCAAG CTCCGAGAGG AGCACATCCT GATGAGAAAT CAGAACTTAG 
     2401 TGGGGCTCAA GCTTCCAGAC CTTAGTGAAG CAGCTGAGCA GGAAAAAGGG CTTCCTTCTG 
     2461 AACTCTCCCC AGCTATTGAG GAAGAAGAGT CAAAGAGTGG CTTAGATGTC ATGCCTAATA 
     2521 TTTCTGATGT GCTGCTGCGC AAACTGCGGG TCCACAGGAG TCTCCCTGGA AGTGCCCCTC 
     2581 CACTCACTGA AAAGGAAGTT GAGAACGTGT TTGTGCAACT GTCCTTGGCC TTTAGAAATG 
     2641 ACAGCTACAC TCTGGAATCT AGAATTAACC AGGCTGAAAG GGAACGCAAC CTGACAGAGG 
     2701 AGAACACTGA GAAAGAACTG GAAAACTTCA AAGCTTCCAT TACGTCCTCA GCTTCACTCT 
     2761 GGCACCACTG TGAGCACCGG GAAACCTACC AGAAGTTGCT GGAGGACATC GCTGTCCTGC 
     2821 ACCGCCTGGC TGCCCGCCTC TCCAGCCGAG CTGAGGTGGT AGGCGCCGTC CGCCAGGAAA 
     2881 AGCGCATGTC GAAAGCAACG GAAGTGATGA TGCAGTATGT GGAGAATCTA AAGAGGACGT 
     2941 ATGAGAAGGA CCATGCGGAG CTCATGGAGT TTAAAAAGCT TGCAAATCAG AATTCAAGCC 
     3001 GCAGCTGTGG CCCCTCTGAA GATGGGGTCC CTCGCACGGC ACGGTCCATG TCCCTCACGC 
     3061 TGGGAAAGAA TATGCCTCGC CGGAGGGTCA GCGTTGCTGT GGTTCCTAAG TTTAATGCCC 
     3121 TGAATCTGCC TGGCCAAACT CCCAGCTCAT CATCCATTCC CTCCTTACCA GCCTTGTCGG 
     3181 AATCACCCAA TGGGAAAGGC AGCCTACCTG TCACTTCAGC ACTGCCTGCA CTTTTGGAAA 
     3241 ATGGAAAGAC AAATGGGGAC CCAGATTGTG AAGCCTCTGC TCCTGCGCTG ACCCTGAGCT 
     3301 GCCTGGAGGA GCTTAGTCAG GAGACCAAGG CCAGGATGGA GGAAGAAGCC TACAGCAAGG 
     3361 GATTCCAAGA AGGTCTAAAG AAGACCAAAG AACTTCAAGA CCTGAAGGAG GAGGAGGAAG 
     3421 AACAGAAGAG TGAGAGTCCT GAGGAACCTG AAGAGGTAGA AGAAACTGAG GAAGAGGAAA 
     3481 AGGGCCCAAG AAGCAGCAAA CTTGAAGAAT TGGTCCATTT CTTACAAGTC ATGTATCCCA 
     3541 AACTGTGTCA GCACTGGCAA GTGATCTGGA TGATGGCTGC AGTGATGCTG GTCTTGACTG 
     3601 TTGTGCTGGG GCTCTACAAT TCCTATAACT CTTGTGCAGA GCAGGCTGAT GGGCCCCTTG 
     3661 GAAGATCCAC TTGCTCGGCA GCCCAGAGGG ACTCCTGGTG GAGCTCAGGA CTCCAGCATG 
     3721 AGCAGCCTAC AGAGCAGACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3781 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3841 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3901 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3961 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     4021 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     4081 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4141 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4201 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4261 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4321 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4381 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4441 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4501 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4561 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4621 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4681 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4741 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4801 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4861 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4921 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4981 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5041 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5101 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5161 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5221 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5281 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5341 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5401 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5461 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5521 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5581 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5641 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5701 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5761 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5821 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5881 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5941 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     6001 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     6061 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6121 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6181 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6241 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6301 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6361 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6421 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6481 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6541 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6601 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6661 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6721 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6781 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6841 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6901 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6961 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     7021 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7081 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7141 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7201 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7261 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7321 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7381 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7441 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7501 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7561 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7621 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7681 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7741 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7801 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7861 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7921 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7981 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     8041 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     8101 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8161 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8221 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8281 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8341 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8401 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8461 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8521 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8581 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8641 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8701 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8761 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8821 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8881 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8941 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     9001 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     9061 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9121 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9181 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9241 TGATTTAACA AAAATTTAAC GCGAATTTT
//