LOCUS       RG219388                9530 bp    DNA     circular  SYN 
DEFINITION  AQPEP (tGFP-tagged) - Human laeverin (AQPEP)
ACCESSION   NM_173800
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4011..4715 
                     /label="GFP"  
     misc_feature    4762..5347 
                     /label="PolyA signal"  
     misc_feature    7439..8084 
                     /label="ColE1"  
     misc_feature    5801..6595 
                     /label="Neo"  
     misc_feature    5432..5734 
                     /label="SV40 ori"  
     misc_feature    8099..8959 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3990..3996 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3990 
                     /label="LVRN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCCCCT TCCAGCTCAG GCTTCTATGT GAGCCGCGCA GTGGCCCTGC 
     1081 TGCTGGCTGG GTTGGTAGCC GCCCTCCTGC TGGCGCTGGC CGTACTCGCC GCCTTGTACG 
     1141 GCCACTGCGA GCGCGTCCCA CCGTCGGAGC TGCCTGGACT CAGGGACTCG GAAGCCGAGT 
     1201 CTTCCCCTCC CCTCAGGCAG AAGCCGACGC CGACCCCGAA ACCCAGCAGT GCACGCGAGC 
     1261 TAGCGGTGAC GACCACCCCG AGCAACTGGC GACCCCCGGG GCCCTGGGAC CAGCTACGCC 
     1321 TGCCGCCCTG GCTCGTGCCG CTGCACTACG ATCTGGAGCT GTGGCCGCAG CTGAGGCCCG 
     1381 ACGAGCTTCC GGCCGGGTCT TTGCCCTTCA CTGGCCGCGT GAACATCACG GTGCGCTGCA 
     1441 CGGTGGCCAC CTCTCGACTG CTGCTGCATA GCCTCTTCCA GGACTGCGAG CGCGCCGAGG 
     1501 TGCGGGGACC CCTTTCCCCG GGCACTGGGA ACGCCACAGT GGGCCGCGTG CCCGTGGACG 
     1561 ACGTGTGGTT CGCGCTGGAC ACGGAATACA TGGTGCTGGA GCTCAGTGAG CCCCTGAAAC 
     1621 CTGGTAGCAG CTACGAGCTG CAGCTTAGCT TCTCGGGCCT GGTGAAGGAA GACCTCAGGG 
     1681 AGGGACTCTT CCTCAACGTC TACACCGACC AGGGCGAGCG CAGGGCCCTG TTAGCGTCCC 
     1741 AGCTGGAACC AACATTTGCC AGGTATGTTT TCCCTTGTTT TGATGAGCCA GCTCTGAAGG 
     1801 CAACTTTTAA TATTACAATG ATTCATCATC CAAGTTATGT GGCCCTTTCC AACATGCCAA 
     1861 AGCTAGGTCA GTCTGAAAAA GAAGATGTGA ATGGAAGCAA ATGGACTGTT ACAACCTTTT 
     1921 CCACTACGCC CCACATGCCA ACTTACTTAG TCGCATTTGT TATATGTGAC TATGACCACG 
     1981 TCAACAGAAC AGAAAGGGGC AAGGAGATAC GCATCTGGGC CCGGAAAGAT GCAATTGCAA 
     2041 ATGGAAGTGC AGACTTTGCT TTGAACATCA CAGGTCCCAT CTTCTCTTTT CTGGAGGATT 
     2101 TGTTTAATAT CAGTTACTCT CTTCCAAAAA CAGATATAAT TGCCTTGCCT AGTTTTGACA 
     2161 ACCATGCAAT GGAAAACTGG GGACTAATGA TATTTGATGA ATCAGGATTG TTGTTGGAAC 
     2221 CAAAAGATCA ACTGACAGAA AAAAAGACTC TGATCTCCTA TGTTGTCTCC CACGAGATTG 
     2281 GACACCAGTG GTTTGGAAAC TTGGTTACCA TGAATTGGTG GAACAATATC TGGCTCAACG 
     2341 AGGGTTTTGC ATCTTATTTT GAGTTTGAAG TAATTAACTA CTTTAATCCT AAACTCCCAA 
     2401 GAAATGAGAT CTTTTTTTCT AACATTTTAC ATAATATCCT CAGAGAAGAT CACGCCCTGG 
     2461 TGACTAGAGC TGTGGCCATG AAGGTGGAAA ATTTCAAAAC AAGTGAAATA CAGGAACTCT 
     2521 TTGACATATT TACTTACAGC AAGGGAGCGT CTATGGCCCG GATGCTTTCT TGTTTCTTGA 
     2581 ATGAGCATTT ATTTGTCAGT GCACTCAAGT CATATTTGAA GACATTTTCC TACTCAAACG 
     2641 CTGAGCAAGA TGATCTATGG AGGCATTTTC AAATGGCCAT AGATGACCAG AGTACAGTTA 
     2701 TTTTGCCAGC AACAATAAAA AACATAATGG ACAGTTGGAC ACACCAGAGT GGTTTTCCAG 
     2761 TGATCACTTT AAATGTGTCT ACTGGCGTCA TGAAACAGGA GCCATTTTAT CTTGAAAACA 
     2821 TTAAAAATCG GACTCTTCTA ACCAGCAATG ACACATGGAT TGTCCCTATT CTTTGGATAA 
     2881 AAAATGGAAC TACACAACCT TTAGTCTGGC TAGATCAAAG CAGCAAAGTA TTCCCAGAAA 
     2941 TGCAAGTTTC AGATTCTGAC CATGACTGGG TGATTTTGAA TTTGAATATG ACTGGATATT 
     3001 ATAGAGTTAA TTATGATAAA TTAGGTTGGA AGAAACTAAA TCAACAACTT GAAAAGGATC 
     3061 CTAAGGCGAT TCCTGTTATT CACAGACTGC AGTTGATTGA TGATGCCTTT TCCTTGTCTA 
     3121 AAAACAATTA TATTGAGATT GAAACAGCAC TTGAGTTAAC CAAGTACCTT GCTGAAGAAG 
     3181 ATGAAATTAT AGTATGGCAT ACAGTCTTGG TAAACTTGGT AACCAGGGAT CTTGTTTCTG 
     3241 AGGTGAACAT CTATGATATA TACTCATTAT TAAAGAGGTA CCTATTAAAG AGACTTAATT 
     3301 TAATATGGAA TATTTATTCA ACTATAATTC GTGAAAATGT GTTGGCATTA CAAGATGACT 
     3361 ACTTAGCTCT AATATCACTG GAAAAACTTT TTGTAACTGC GTGTTGGTTG GGCCTTGAAG 
     3421 ACTGCCTTCA GCTGTCAAAA GAACTTTTCG CAAAATGGGT GGATCATCCA GAAAATGAAA 
     3481 TACCTTATCC AATTAAAGAT GTGGTTTTAT GTTATGGCAT TGCCTTGGGA AGTGATAAAG 
     3541 AGTGGGACAT CTTGTTAAAT ACTTACACTA ATACAACAAA CAAAGAAGAA AAGATTCAAC 
     3601 TTGCTTATGC AATGAGCTGC AGCAAAGACC CATGGATACT TAACAGATAT ATGGAGTATG 
     3661 CCATCAGCAC ATCTCCATTC ACTTCTAATG AAACAAATAT AATTGAGGTT GTGGCTTCAT 
     3721 CTGAAGTTGG CCGGTATGTC GCAAAAGACT TCTTAGTCAA CAACTGGCAA GCTGTGAGTA 
     3781 AAAGGTATGG AACACAATCA TTGATTAATC TAATATATAC AATAGGGAGA ACCGTAACTA 
     3841 CAGATTTACA GATTGTGGAG CTGCAGCAGT TTTTCAGTAA CATGTTGGAG GAACACCAGA 
     3901 GGATCAGAGT TCATGCCAAC TTACAGACAA TAAAGAATGA AAATCTGAAA AACAAGAAGC 
     3961 TAAGTGCCAG GATAGCTGCG TGGCTAAGGA GAAACACAAC GCGTACGCGG CCGCTCGAGA 
     4021 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     4081 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     4141 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     4201 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     4261 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     4321 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     4381 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     4441 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     4501 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     4561 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     4621 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     4681 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5461 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     5521 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     5581 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     5641 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     5701 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     5761 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     5821 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     5881 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     5941 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     6001 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     6061 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     6121 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     6181 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     6241 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     6301 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     6361 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     6421 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     6481 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     6541 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     6601 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     6661 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     6721 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     6781 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     6841 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     6901 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     6961 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     7021 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     7081 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     7141 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     7201 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     7261 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     7321 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     7381 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     7441 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     7501 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     7561 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     7621 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     7681 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     7741 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     7801 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     7861 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     7921 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     7981 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     8041 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     8101 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     8161 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     8221 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     8281 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     8341 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     8401 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     8461 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     8521 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     8581 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     8641 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     8701 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     8761 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     8821 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     8881 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     8941 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     9001 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     9061 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     9121 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     9181 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     9241 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     9301 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     9361 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     9421 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     9481 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//