LOCUS       RG219053                9482 bp    DNA     circular  SYN 
DEFINITION  PITPNM3 (tGFP-tagged) - Human PITPNM family member 3 (PITPNM3), transcript variant 1
ACCESSION   NM_031220
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3963..4667 
                     /label="GFP"  
     misc_feature    4714..5299 
                     /label="PolyA signal"  
     misc_feature    7391..8036 
                     /label="ColE1"  
     misc_feature    5753..6547 
                     /label="Neo"  
     misc_feature    5384..5686 
                     /label="SV40 ori"  
     misc_feature    8051..8911 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3942..3948 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3942 
                     /label="PITPNM3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAGGCG GGCCGTGCAG GTGGTCCTCC CCCGGGCGGC GGTGCCCCCT 
     1081 GGCACCTTCG AAATGTCCTC AGTGACTCTG TGGAGAGCTC AGATGATGAA TTCTTTGATG 
     1141 CCAGAGAGGA GATGGCTGAA GGGAAGAATG CCATCCTCAT TGGGATGAGC CAGTGGAACT 
     1201 CCAATGACCT CGTGGAGCAG ATCGAGACCA TGGGGAAACT GGACGAGCAT CAAGGAGAAG 
     1261 GGACCGCGCC GTGCACATCC AGCATCCTCC AGGAGAAGCA GCGAGAACTG TACCGGGTTT 
     1321 CCTTGAGAAG ACAGAGGTTC CCAGCCCAGG GAAGCATCGA GATCCACGAA GACAGCGAGG 
     1381 AAGGCTGCCC GCAGCGCTCC TGCAAGACAC ATGTCCTCCT GCTGGTCCTG CATGGGGGAA 
     1441 ACATCCTGGA CACGGGTGCC GGGGACCCGT CCTGCAAGGC AGCCGACATC CACACCTTCA 
     1501 GCTCCGTGCT GGAGAAGGTC ACACGAGCCC ATTTCCCTGC TGCCCTGGGC CACATCCTCA 
     1561 TCAAGTTCGT CCCCTGTCCT GCCATCTGCT CTGAGGCTTT CTCGCTTGTC TCTCACCTGA 
     1621 ACCCCTACAG CCACGATGAG GGCTGCCTCA GCAGCAGCCA GGACCACGTC CCTCTGGCCG 
     1681 CCCTTCCCCT GTTGGCCATC TCCTCCCCGC AGTACCAGGA TGCTGTCGCC ACCGTCATCG 
     1741 AGCGAGCCAA CCAGGTCTAC AGAGAGTTCC TGAAGTCCTC TGATGGGATT GGCTTCAGTG 
     1801 GGCAGGTGTG TCTCATCGGG GACTGTGTGG GGGGCCTCCT GGCCTTCGAT GCCATCTGCT 
     1861 ACAGTGCGGG GCCCTCAGGG GACAGCCCTG CCAGCAGCAG CCGGAAGGGG AGCATCAGCA 
     1921 GCACCCAGGA CACCCCAGTC GCGGTGGAGG AAGATTGCAG CCTGGCCAGC AGCAAGCGTC 
     1981 TCAGCAAAAG CAACATTGAC ATCTCCAGTG GGTTGGAGGA TGAGGAGCCC AAGAGGCCGT 
     2041 TGCCGCGGAA ACAGAGCGAC TCCTCCACCT ATGACTGCGA GGCCATCACC CAGCACCATG 
     2101 CCTTCCTCTC AAGCATCCAC TCCAGCGTGC TAAAGGATGA GTCTGAGACC CCGGCGGCTG 
     2161 GGGGGCCGCA GCTCCCTGAG GTCAGCCTGG GCCGCTTTGA CTTCGATGTG TCCGACTTCT 
     2221 TCCTCTTCGG CTCGCCACTG GGCCTGGTCC TGGCCATGCG GAGGACGGTG CTGCCTGGGC 
     2281 TGGACGGCTT CCAGGTGCGT CCTGCCTGCA GCCAGGTCTA CAGCTTCTTC CATTGCGCAG 
     2341 ACCCCTCTGC CTCACGGCTC GAGCCACTGC TGGAGCCCAA GTTCCACCTG GTGCCGCCTG 
     2401 TCAGCGTGCC TCGCTACCAG AGGTTCCCAC TGGGCGATGG GCAGTCCCTC CTCCTCGCTG 
     2461 ATGCCCTACA CACCCACAGC CCCCTCTTCC TGGAGGGCAG CTCCCGGGAC AGCCCGCCAC 
     2521 TTCTGGATGC CCCTGCCTCG CCCCCTCAGG CCTCGAGGTT CCAGCGCCCA GGACGGAGGA 
     2581 TGAGCGAGGG GAGCTCCCAC AGCGAGAGCT CGGAGTCCTC GGACAGCATG GCACCCGTGG 
     2641 GTGCCTCCCG CATCACAGCC AAGTGGTGGG GAAGCAAGAG GATCGACTAT GCCCTGTACT 
     2701 GCCCTGATGT CCTCACGGCC TTCCCCACCG TGGCCCTGCC CCACCTCTTC CACGCCAGTT 
     2761 ACTGGGAGTC CACAGACGTG GTGGCCTTCA TCCTGAGACA GGTAATGCGC TATGAGAGCG 
     2821 TGAACATCAA GGAAAGCGCC CGCCTGGACC CTGCAGCACT GAGTCCTGCC AACCCCCGGG 
     2881 AGAAGTGGCT TCGTAAGCGG ACTCAGGTCA AGCTGAGGAA TGTCACGGCT AATCACCGGG 
     2941 CCAATGATGT GATTGCTGCT GAAGATGGCC CCCAGGTCCT GGTGGGGCGG TTCATGTACG 
     3001 GGCCCCTCGA CATGGTGGCT CTGACTGGAG AGAAGGTGGA CATCCTAGTA ATGGCAGAGC 
     3061 CATCCTCAGG CCGCTGGGTA CACCTGGACA CAGAGATCAC CAACAGCAGT GGTCGCATCA 
     3121 CATACAATGT GCCGCGGCCC CGGCGCCTGG GGGTTGGTGT CTATCCTGTG AAGATGGTCG 
     3181 TCAGGGGCGA CCAGACCTGT GCCATGAGCT ACCTCACGGT GTTGCCCAGG GGCATGGAGT 
     3241 GTGTAGTGTT CAGCATTGAT GGGTCCTTCG CGGCCAGCGT GTCTATCATG GGAAGCGACC 
     3301 CCAAGGTCCG GCCGGGTGCA GTGGATGTTG TCCGGCACTG GCAGGACTTG GGCTACATGA 
     3361 TCCTTTACAT CACGGGACGG CCGGACATGC AGAAGCAGCG GGTGGTGTCG TGGCTGTCCC 
     3421 AGCACAACTT CCCACAGGGC ATGATCTTCT TCTCCGACGG GCTGGTGCAT GACCCGCTGC 
     3481 GGCAGAAGGC CATCTTCCTG CGCAACCTCA TGCAGGAGTG CTTCATCAAA ATCAGTGCGG 
     3541 CCTATGGCTC CACGAAGGAC ATCTCTGTCT ACAGCGTGCT GGGCCTGCCT GCCTCCCAGA 
     3601 TCTTCATTGT GGGCCGGCCC ACCAAGAAGT ACCAAACCCA GTGCCAGTTC CTGAGCGAGG 
     3661 GCTACGCCGC ACACCTGGCC GTGCTGGAGG CCAGCCACCG CTCACGCCCA AAGAAGAACA 
     3721 ACTCGCGCAT GATCCTGCGC AAGGGCAGCT TCGGGCTGCA CGCGCAGCCA GAGTTCCTGC 
     3781 GGAAGCGCAA CCACCTGCGC AGAACCATGT CAGTGCAGCA GCCCGACCCG CCCGCCGCCA 
     3841 ACCCCAAGCC CGAGCGGGCC CAGAGCCAGC CCGAGTCGGA CAAAGACCAC GAGCGGCCGC 
     3901 TGCCGGCGCT CAGCTGGGCG CGTGGGCCCC CCAAGTTCGA GTCGGTGCCC ACGCGTACGC 
     3961 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     4021 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     4081 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     4141 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     4201 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4261 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4321 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4381 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4441 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4501 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4561 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4621 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5401 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5461 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5521 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5581 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5641 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5701 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5761 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5821 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5881 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5941 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     6001 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     6061 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     6121 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     6181 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6241 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6301 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6361 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6421 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6481 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6541 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6601 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6661 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6721 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6781 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6841 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6901 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6961 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     7021 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     7081 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     7141 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     7201 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7261 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7321 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7381 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7441 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7501 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7561 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7621 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7681 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7741 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7801 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7861 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7921 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7981 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     8041 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     8101 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     8161 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     8221 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8281 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8341 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8401 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8461 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8521 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8581 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8641 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8701 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8761 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8821 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8881 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8941 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     9001 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     9061 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     9121 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     9181 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9241 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9301 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9361 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9421 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9481 TT
//