LOCUS       RG218886                9422 bp    DNA     circular  SYN 
DEFINITION  INPP4A (tGFP-tagged) - Human inositol polyphosphate-4-phosphatase, type I, 107kDa (INPP4A), transcript variant b
ACCESSION   NM_001566
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3903..4607 
                     /label="GFP"  
     misc_feature    4654..5239 
                     /label="PolyA signal"  
     misc_feature    7331..7976 
                     /label="ColE1"  
     misc_feature    5693..6487 
                     /label="Neo"  
     misc_feature    5324..5626 
                     /label="SV40 ori"  
     misc_feature    7991..8851 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3882..3888 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3882 
                     /label="INPP4A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAGCAAGA GAGCACAGCC CTCGCCATGG TGCCAGGGCC CGTGCAATGC 
     1081 AGCGGGCTTC CACCATCGAC GTGGCGGCCG ACATGCTGGG CCTCTCTCTG GCAGGAAATA 
     1141 TACAAGACCC AGATGAGCCC ATTTTAGAAT TTAGCTTAGC TTGCAGTGAG CTGCATACTC 
     1201 CATCGCTAGA TCGAAAGCCA AATAGTTTTG TTGCGGTGAG TGTCACCACC CCTCCTCAGG 
     1261 CATTCTGGAC GAAGCATGCA CAGACGGAGA TCATTGAGGG AACCAACAAT CCTATATTTC 
     1321 TAAGCAGTAT TGCCTTCTTT CAAGACTCTC TTATCAATCA GATGACACAA GTCAAACTCT 
     1381 CCGTGTATGA TGTCAAAGAT AGATCTCAGG GAACAATGTA TTTACTGGGC TCTGGAACGT 
     1441 TCATTGTCAA AGATCTGCTC CAGGACAGGC ATCATAGGTT GCATTTAACA CTAAGGTCTG 
     1501 CAGAGAGTGA CCGTGTAGGT AACATCACCG TGATTGGCTG GCAGATGGAG GAGAAGTCAG 
     1561 ACCAACGGCC CCCTGTGACC CGGTCTGTGG ACACTGTCAA TGGGAGGATG GTTCTTCCTG 
     1621 TCGATGAGAG CTTGACGGAG GCGTTAGGAA TCCGATCCAA ATACGCTTCA TTGCGAAAGG 
     1681 ACACTTTGCT GAAATCGGTG TTCGGTGGTG CCATCTGCCG CATGTACCGG TTTCCAACCA 
     1741 CTGATGGTAA CCATTTGCGG ATCCTGGAGC AGATGGCAGA GAGCGTGCTC TCCCTGCACG 
     1801 TGCCCCGGCA GTTCGTGAAG CTCCTACTAG AGGAAGATGC AGCCAGAGTG TGTGAGCTGG 
     1861 AGGAGCTGGG AGAGCTGTCC CCTTGCTGGG AGAGCCTCCG GCGCCAAATT GTCACCCAGT 
     1921 ACCAGACCAT CATCCTCACA TACCAGGAGA ACCTGACCGA CCTCCATCAG TACAGAGGGC 
     1981 CCTCGTTTAA AGCAAGCAGT TTGAAAGCAG ATAAAAAGTT AGAATTTGTT CCCACAAACT 
     2041 TGCACATACA AAGGATGAGA GTTCAAGACG ATGGAGGATC AGATCAGAAC TACGACATCG 
     2101 TCACCATTGG GGCGCCAGCA GCACACTGCC AAGGTTTTAA GTCAGGAGGT CTCCGCAAAA 
     2161 AGCTGCACAA ATTTGAAGAG ACCAAGAAAC ATTTTGAGGA GTGTTGTACA TCATCTGGCT 
     2221 GCCAGTCCAT AATCTACATA CCCCAGGATG TTGTCAGAGC CAAGGAGATC ATCGCCCAGA 
     2281 TCAACACCCT GAAAACCCAA GTGAGTTACT ACGCAGAGCG GCTGTCAAGG GCAGCCAAGG 
     2341 ACAGGTCTGC CACTGGCCTT GAGAGGACAC TCGCCATCTT GGCAGACAAG ACACGGCAGC 
     2401 TGGTCACGGT CTGCGACTGC AAGCTCCTGG CCAACTCCAT CCATGGGCTG AACGCTGCAC 
     2461 GGCCTGACTA CATTGCCTCC AAGGCCTCTC CCACTTCGAC TGAGGAGGAG CAGGTGATGC 
     2521 TTAGAAATGA CCAGGACACC CTCATGGCCC GGTGGACAGG GAGAAACAGC CGATCTTCCC 
     2581 TGCAGGTGGA CTGGCACGAG GAGGAGTGGG AGAAAGTGTG GCTGAACGTG GACAAGAGCC 
     2641 TAGAGTGCAT CATTCAGCGT GTGGACAAGC TGCTGCAGAA GGAGCGGCTG CATGGCGAGG 
     2701 GCTGTGAGGA TGTCTTCCCC TGTGCAGGCA GCTGCACCAG CAAGAAAGAT TGCAGTCCCC 
     2761 CTCCTGAAGA GTCCAGCCCA GGTGAATGGA GTGAGGCCCT TTACCCGCTG CTGACCACTC 
     2821 TCACCGACTG CGTGGCCATG ATGAGTGACA AGGCCAAGAA GGCCATGGTA TTCCTGCTCA 
     2881 TGCAGGACAG CGCGCCCACC ATAGCCACCT ACCTGAGCCT GCAGTACCGC CGTGACGTGG 
     2941 TCTTCTGCCA GACGCTGACC GCCCTCATCT GCGGCTTCAT CATTAAGCTG AGGAACTGCC 
     3001 TGCATGACGA CGGCTTCCTG CGCCAGCTCT ACACCATCGG GCTGCTGGCC CAGTTCGAGA 
     3061 GCCTGCTGAG CACCTACGGG GAGGAGCTGG CAATGCTGGA GGACATGAGC CTTGGGATCA 
     3121 TGGACTTGAG GAACGTGACC TTCAAAGTCA CTCAGGCCAC TTCCAGCGCC TCCGCAGACA 
     3181 TGCTGCCCGT CATCACAGGA AATCGCGACG GGTTTAACGT GCGGGTCCCT CTGCCGGGCC 
     3241 CGCTGTTTGA CGCCTTGCCC CGGGAGATCC AGAGTGGCAT GCTGCTGCGA GTGCAGCCCG 
     3301 TCCTCTTCAA CGTGGGCATC AATGAGCAGC AGACACTGGC CGAGAGGTTT GGCGATACGT 
     3361 CTTTACAAGA AGTCATCAAC GTGGAGAGTT TGGTGCGGTT AAATTCCTAC TTTGAGCAGT 
     3421 TTAAGGAAGT TTTGCCTGAG GATTGCCTGC CTCGGTCTCG CAGTCAGACG TGCCTGCCAG 
     3481 AGCTGCTGCG GTTTCTGGGT CAGAACGTGC ATGCCCGGAA GAATAAGAAC GTCGACATTC 
     3541 TCTGGCAAGC TGCTGAGATC TGCCGCCGCC TTAATGGGGT CCGGTTCACC AGCTGCAAGA 
     3601 GCGCTAAGGA CCGTACAGCC ATGTCGGTGA CACTGGAGCA GTGCCTGATC CTGCAACACG 
     3661 AGCATGGCAT GGCCCCGCAG GTCTTCACCC AGGCCCTGGA GTGCATGCGC AGCATTGGAA 
     3721 CACGGGAGGT AGTCACCCAG AAAAACTTGA GCGGCCTGGT GCCCATCCGA GACTTAAGGC 
     3781 TAGACCCCAG CCTCCTCTGT TCCATCCCTT TATTAGCTCT GAGCCCCAAT TTACTGATTG 
     3841 TGTGGCTCTT TCTGAGCATA GCATACCTGG TGACCAAATT GCGTTGCAAA ACGCGTACGC 
     3901 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3961 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     4021 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     4081 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     4141 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4201 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4261 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4321 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4381 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4441 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4501 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4561 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4621 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4681 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4741 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4801 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4861 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4921 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4981 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5041 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5101 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5161 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5221 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5281 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5341 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5401 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5461 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5521 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5581 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5641 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5701 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5761 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5821 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5881 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5941 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     6001 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     6061 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     6121 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6181 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6241 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6301 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6361 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6421 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6481 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6541 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6601 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6661 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6721 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6781 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6841 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6901 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6961 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     7021 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     7081 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     7141 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7201 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7261 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7321 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7381 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7441 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7501 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7561 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7621 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7681 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7741 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7801 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7861 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7921 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7981 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     8041 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     8101 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     8161 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8221 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8281 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8341 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8401 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8461 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8521 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8581 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8641 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8701 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8761 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8821 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8881 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8941 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     9001 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     9061 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     9121 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9181 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9241 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9301 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9361 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9421 TT
//