LOCUS       RG218556                9386 bp    DNA     circular  SYN 
DEFINITION  INTU (tGFP-tagged) - Human inturned planar cell polarity effector homolog (Drosophila) (INTU)
ACCESSION   NM_015693
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3867..4571 
                     /label="GFP"  
     misc_feature    4618..5203 
                     /label="PolyA signal"  
     misc_feature    7295..7940 
                     /label="ColE1"  
     misc_feature    5657..6451 
                     /label="Neo"  
     misc_feature    5288..5590 
                     /label="SV40 ori"  
     misc_feature    7955..8815 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3846..3852 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3846 
                     /label="INTU"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCTGTG GCTTCGTGCG ATTCGCGTCC GAGCTCAGAC GAGCTCCCTG 
     1081 GAGACCCCTC TTCACAAGAA GAAGATGAGG ACTATGATTT TGAAGATCGG GTCAGCGACT 
     1141 CGGGTTCATA TTCCTCAGCG AGTAGCGATT ATGATGATCT TGAGCCTGAA TGGCTGGACA 
     1201 GTGTGCAGAA AAATGGAGAG CTGTTTTATT TGGAATTGAG TGAGGATGAA GAAGAAAGCC 
     1261 TCCTTCCTGA GACACCAACT GTGAACCATG TCAGGTTCAG TGAAAATGAG ATTATCATTG 
     1321 AAGATGACTA CAAAGAAAGA AAAAAGTATG AACCCAAACT CAAGCAGTTT ACCAAAATTT 
     1381 TAAGAAGGAA AAGACTTTTA CCCAAGCGCT GCAATAAAAA AAATAGCAAT GACAATGGAC 
     1441 CAGTATCCAT TCTAAAGCAT CAGTCCAATC AGAAGACAGG AGTCATTGTC CAACAGCGGT 
     1501 ACAAAGATGT GAATGTTTAT GTAAACCCCA AAAAGCTAAC TGTTATCAAA GCCAAAGAGC 
     1561 AGCTCAAGCT TCTGGAAGTG CTGGTTGGAA TTATTCATCA GACCAAGTGG AGCTGGAGAA 
     1621 GAACCGGAAA GCAGGGTGAT GGAGAGAGGC TTGTGGTTCA TGGCCTGCTG CCAGGGGGAT 
     1681 CTGCTATGAA GAGCGGTCAG GTACTCATTG GTGATGTCCT TGTTGCTGTG AATGATGTCG 
     1741 ATGTTACTAC TGAAAACATC GAGAGAGTTC TGTCTTGCAT TCCTGGACCT ATGCAGGTGA 
     1801 AACTGACATT TGAAAATGCA TATGATGTGA AAAGGGAGAC ATCCCATCCA AGACAGAAAA 
     1861 AGACACAGTC CAACACAAGT GATTTAGTCA AGCTTCTCTG GGGAGAAGAG GTTGAAGGTA 
     1921 TCCAGCAGAG TGGCCTAAAC ACTCCTCATA TCATTATGTA TCTCACACTA CAGCTCGACT 
     1981 CAGAAACCTC AAAGGAAGAG CAGGAAATTC TTTATCATTA TCCAATGTCT GAAGCATCTC 
     2041 AGAAACTTAA AAGTGTGAGA GGGATTTTTC TCACACTCTG TGACATGCTG GAAAACGTAA 
     2101 CTGGGACACA AGTTACTAGT TCATCCCTCC TTTTAAATGG AAAACAAATT CATGTGGCTT 
     2161 ATTGGAAAGA ATCTGACAAG TTGTTGCTAA TTGGCCTGCC TGCTGAAGAA GTTCCTCTTC 
     2221 CTCGTCTAAG GAACATGATA GAAAATGTCA TCCAAACCTT AAAATTTATG TATGGTTCTT 
     2281 TAGATAGTGC CTTTTGCCAG ATTGAGAATG TTCCTCGTTT GGATCATTTT TTTAACTTGT 
     2341 TCTTTCAAAG AGCACTTCAG CCTGCGAAAC TGCATTCCAG CGCCAGTCCC AGTGCTCAGC 
     2401 AGTACGATGC TTCCAGTGCA GTACTTTTAG ACAACCTCCC TGGAGTCCGG TGGCTCACAC 
     2461 TTCCACTGGA AATCAAGATG GAATTAGACA TGGCATTAAG TGACTTGGAG GCTGCAGATT 
     2521 TTGCAGAACT GTCCGAGGAT TACTATGACA TGAGGCGGCT GTATACAATT TTGGGGTCTT 
     2581 CTCTATTTTA CAAGGGTTAT TTGATATGCA GTCATTTGCC CAAGGATGAT CTTATTGATA 
     2641 TTGCCGTATA CTGTCGCCAC TATTGCCTGC TGCCTTTAGC AGCAAAACAA AGAATTGGTC 
     2701 AGTTGATCAT ATGGAGAGAA GTGTTTCCTC AGCATCACCT CCGACCTTTG GCAGACTCAA 
     2761 GCACTGAAGT CTTTCCGGAA CCTGAAGGAA GATATTTTTT GCTAGTTGTT GGCTTGAAAC 
     2821 ATTATATGCT ATGTGTACTA TTAGAAGCTG GAGGTTGCGC ATCCAAAGCT ATTGGGAGTC 
     2881 CTGGACCAGA CTGTGTATAT GTGGATCAAG TCAAAACAAC TCTTCACCAG CTGGATGGAG 
     2941 TAGATTCTCG CATAGATGAA CGGCTAGCAT CTTCTCCAGT CCCCTGTTTG TCTTGTGCTG 
     3001 ACTGGTTCCT TACTGGATCA CGTGAAAAAA CAGATAGCTT GACCACTTCG CCTATTCTCA 
     3061 GTAGGCTACA AGGTACTTCC AAAGTAGCAA CTTCTCCAAC ATGCAGAAGA ACGCTTTTTG 
     3121 GTGACTATTC CTTAAAGACA CGCAAGCCTA GTCCTTCCTG TAGTAGTGGA GGATCTGACA 
     3181 ATGGTTGTGA AGGTGGAGAA GATGATGGCT TTAGCCCCCA TACTACACCG GATGCAGTAC 
     3241 GGAAGCAAAG AGAATCTCAG GGCTCTGATG GTTTAGAAGA AAGTGGGACC TTGCTTAAGG 
     3301 TCACTAAAAA GAAGTCTACT CTTCCAAATC CATTTCATTT GGGAAACTTG AAAAAGGACC 
     3361 TTCCAGAAAA AGAATTAGAA ATATATAACA CAGTGAAACT GACATCTGGT CCTGAGAACA 
     3421 CACTTTTCCA CTACGTTGCC TTAGAAACAG TGCAAGGAAT CTTTATTACT CCTACCCTTG 
     3481 AAGAGGTGGC ACAGCTAAGT GGCTCTATCC ACCCTCAGCT AATAAAGAAT TTCCATCAGT 
     3541 GTTGTCTTTC CATTCGTGCA GTTTTCCAAC AGACATTGGT GGAAGAGAAA AAGAAAGGAC 
     3601 TAAATAGTGG AGACCATTCA GATTCTGCAA AGTCAGTGTC TTCTCTTAAC CCTGTTAAAG 
     3661 AACATGGTGT GTTGTTTGAA TGTTCACCTG GAAACTGGAC TGATCAGAAA AAAGCACCAC 
     3721 CAGTTATGGC TTACTGGGTA GTAGGGAGAC TTTTTCTTCA TCCAAAACCT CAAGAACTTT 
     3781 ATGTCTGTTT TCATGACTCA GTCACAGAAA TTGCCATTGA AATAGCTTTT AAATTGTTCT 
     3841 TTGGGTTAAC CTTGACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3901 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3961 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     4021 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     4081 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     4141 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     4201 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     4261 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4321 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4381 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4441 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4501 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4561 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4621 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4681 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4741 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4801 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4861 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4921 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4981 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5041 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5101 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5161 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5221 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     5281 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5341 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5401 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5461 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5521 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5581 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5641 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5701 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5761 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5821 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5881 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5941 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     6001 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     6061 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     6121 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     6181 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     6241 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6301 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6361 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6421 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6481 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6541 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6601 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6661 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6721 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6781 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6841 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6901 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6961 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     7021 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     7081 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     7141 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     7201 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7261 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7321 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7381 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7441 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7501 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7561 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7621 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7681 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7741 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7801 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7861 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7921 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7981 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     8041 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     8101 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     8161 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     8221 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     8281 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8341 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8401 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8461 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8521 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8581 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8641 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8701 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8761 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8821 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8881 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8941 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     9001 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     9061 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     9121 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     9181 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     9241 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9301 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9361 TTTAACAAAA ATTTAACGCG AATTTT
//