LOCUS       RG218267                9395 bp    DNA     circular  SYN 
DEFINITION  UNC5B (GFP-tagged) - Human unc-5 homolog B (C. elegans) (UNC5B)
ACCESSION   NM_170744
VERSION     NM_170744.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3876..4580 
                     /label="GFP"  
     misc_feature    4627..5212 
                     /label="PolyA signal"  
     misc_feature    7304..7949 
                     /label="ColE1"  
     misc_feature    5666..6460 
                     /label="Neo"  
     misc_feature    5297..5599 
                     /label="SV40 ori"  
     misc_feature    7964..8824 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3855..3861 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3855 
                     /label="UNC5B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCCCGG AGCGGAGCTC GGGGCGCGCT GCTGCTGGCA CTGCTGCTCT 
     1081 GCTGGGACCC GAGGCTGAGC CAAGCAGGCA CTGATTCTGG CAGCGAGGTG CTCCCTGACT 
     1141 CCTTCCCGTC AGCGCCAGCA GAGCCGCTGC CCTACTTCCT GCAGGAGCCA CAGGACGCCT 
     1201 ACATTGTGAA GAACAAGCCT GTGGAGCTCC GCTGCCGCGC CTTCCCCGCC ACACAGATCT 
     1261 ACTTCAAGTG CAACGGCGAG TGGGTCAGCC AGAACGACCA CGTCACACAG GAAGGCCTGG 
     1321 ATGAGGCCAC CGGCCTGCGG GTGCGCGAGG TGCAGATCGA GGTGTCGCGG CAGCAGGTGG 
     1381 AGGAGCTCTT TGGGCTGGAG GATTACTGGT GCCAGTGCGT GGCCTGGAGC TCCGCGGGCA 
     1441 CCACCAAGAG TCGCCGAGCC TACGTCCGCA TCGCCTACCT GCGCAAGAAC TTCGATCAGG 
     1501 AGCCTCTGGG CAAGGAGGTG CCCCTGGACC ATGAGGTTCT CCTGCAGTGC CGCCCGCCGG 
     1561 AGGGGGTGCC TGTGGCCGAG GTGGAATGGC TCAAGAATGA GGATGTCATC GACCCCACCC 
     1621 AGGACACCAA CTTCCTGCTC ACCATCGACC ACAACCTCAT CATCCGCCAG GCCCGCCTGT 
     1681 CGGACACTGC CAACTATACC TGCGTGGCCA AGAACATCGT GGCCAAACGC CGGAGCACCA 
     1741 CTGCCACCGT CATCGTCTAC GTGAATGGCG GCTGGTCCAG CTGGGCAGAG TGGTCACCCT 
     1801 GCTCCAACCG CTGTGGCCGA GGCTGGCAGA AGCGCACCCG GACCTGCACC AACCCCGCTC 
     1861 CACTCAACGG AGGGGCCTTC TGCGAGGGCC AGGCATTCCA GAAGACCGCC TGCACCACCA 
     1921 TCTGCCCAGT CGATGGGGCG TGGACGGAGT GGAGCAAGTG GTCAGCCTGC AGCACTGAGT 
     1981 GTGCCCACTG GCGTAGCCGC GAGTGCATGG CGCCCCCACC CCAGAACGGA GGCCGTGACT 
     2041 GCAGCGGGAC GCTGCTCGAC TCTAAGAACT GCACAGATGG GCTGTGCATG CAAAATAAGA 
     2101 AAACTCTAAG CGACCCCAAC AGCCACCTGC TGGAGGCCTC AGGGGATGCG GCGCTGTATG 
     2161 CGGGGCTCGT GGTGGCCATC TTCGTGGTCG TGGCAATCCT CATGGCGGTG GGGGTGGTGG 
     2221 TGTACCGCCG CAACTGCCGT GACTTCGACA CAGACATCAC TGACTCATCT GCTGCCCTGA 
     2281 CTGGTGGTTT CCACCCCGTC AACTTTAAGA CGGCAAGGCC CAGCAACCCG CAGCTCCTAC 
     2341 ACCCCTCTGT GCCTCCTGAC CTGACAGCCA GCGCCGGCAT CTACCGCGGA CCCGTGTATG 
     2401 CCCTGCAGGA CTCCACCGAC AAAATCCCCA TGACCAACTC TCCTCTGCTG GACCCCTTAC 
     2461 CCAGCCTTAA GGTCAAGGTC TACAGCTCCA GCACCACGGG CTCTGGGCCA GGCCTGGCAG 
     2521 ACGGGGCTGA CCTGCTGGGG GTCTTGCCGC CTGGCACATA CCCTAGCGAT TTCGCCCGGG 
     2581 ACACCCACTT CCTGCACCTG CGCAGCGCCA GCCTCGGTTC CCAGCAGCTC TTGGGCCTGC 
     2641 CCCGAGACCC AGGGAGCAGC GTCAGCGGCA CCTTTGGCTG CCTGGGTGGG AGGCTCAGCA 
     2701 TCCCCGGCAC AGGGGTCAGC TTGCTGGTGC CCAATGGAGC CATTCCCCAG GGCAAGTTCT 
     2761 ACGAGATGTA TCTACTCATC AACAAGGCAG AAAGTACCCT CCCGCTTTCA GAAGGGACCC 
     2821 AGACAGTATT GAGCCCCTCG GTGACCTGTG GACCCACAGG CCTCCTGCTG TGCCGCCCCG 
     2881 TCATCCTCAC CATGCCCCAC TGTGCCGAAG TCAGTGCCCG TGACTGGATC TTTCAGCTCA 
     2941 AGACCCAGGC CCACCAGGGC CACTGGGAGG AGGTGGTGAC CCTGGATGAG GAGACCCTGA 
     3001 ACACACCCTG CTACTGCCAG CTGGAGCCCA GGGCCTGTCA CATCCTGCTG GACCAGCTGG 
     3061 GCACCTACGT GTTCACGGGC GAGTCCTATT CCCGCTCAGC AGTCAAGCGG CTCCAGCTGG 
     3121 CCGTCTTCGC CCCCGCCCTC TGCACCTCCC TGGAGTACAG CCTCCGGGTC TACTGCCTGG 
     3181 AGGACACGCC TGTAGCACTG AAGGAGGTGC TGGAGCTGGA GCGGACTCTG GGCGGATACT 
     3241 TGGTGGAGGA GCCGAAACCG CTAATGTTCA AGGACAGTTA CCACAACCTG CGCCTCTCCC 
     3301 TCCATGACCT CCCCCATGCC CATTGGAGGA GCAAGCTGCT GGCCAAATAC CAGGAGATCC 
     3361 CCTTCTATCA CATTTGGAGT GGCAGCCAGA AGGCCCTCCA CTGCACTTTC ACCCTGGAGA 
     3421 GGCACAGCTT GGCCTCCACA GAGCTCACCT GCAAGATCTG CGTGCGGCAA GTGGAAGGGG 
     3481 AGGGCCAGAT ATTCCAGCTG CATACCACTC TGGCAGAGAC ACCTGCTGGC TCCCTGGACA 
     3541 CTCTCTGCTC TGCCCCTGGC AGCACTGTCA CCACCCAGCT GGGACCTTAT GCCTTCAAGA 
     3601 TCCCACTGTC CATCCGCCAG AAGATATGCA ACAGCCTAGA TGCCCCCAAC TCACGGGGCA 
     3661 ATGACTGGCG GATGTTAGCA CAGAAGCTCT CTATGGACCG GTACCTGAAT TACTTTGCCA 
     3721 CCAAAGCGAG CCCCACGGGT GTGATCCTGG ACCTCTGGGA AGCTCTGCAG CAGGACGATG 
     3781 GGGACCTCAA CAGCCTGGCG AGTGCCTTGG AGGAGATGGG CAAGAGTGAG ATGCTGGTGG 
     3841 CTGTGGCCAC CGACGGGGAC TGCACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3901 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     3961 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     4021 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     4081 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     4141 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     4201 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     4261 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     4321 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4381 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4441 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4501 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4561 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4621 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4681 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4741 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4801 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4861 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4921 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4981 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5041 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5101 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5161 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5221 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     5281 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5341 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5401 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5461 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5521 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5581 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5641 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5701 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5761 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5821 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5881 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     5941 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     6001 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     6061 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     6121 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     6181 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     6241 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     6301 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6361 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6421 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6481 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6541 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6601 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6661 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6721 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6781 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6841 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6901 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     6961 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     7021 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     7081 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     7141 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     7201 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     7261 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     7321 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7381 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7441 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7501 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7561 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7621 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7681 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7741 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7801 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7861 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7921 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     7981 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     8041 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     8101 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     8161 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     8221 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     8281 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     8341 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8401 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8461 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8521 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8581 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8641 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8701 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8761 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8821 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8881 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     8941 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     9001 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     9061 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     9121 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     9181 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     9241 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     9301 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9361 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//