LOCUS RG218131 8981 bp DNA circular SYN DEFINITION SPON1 (GFP-tagged) - Human spondin 1, extracellular matrix protein (SPON1) ACCESSION NM_006108 VERSION NM_006108.1 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3462..4166 /label="GFP" misc_feature 4213..4798 /label="PolyA signal" misc_feature 6890..7535 /label="ColE1" misc_feature 5252..6046 /label="Neo" misc_feature 4883..5185 /label="SV40 ori" misc_feature 7550..8410 /label="Amp" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3441..3447 /label="Restriction Enzyme" misc_feature 1020..3441 /label="SPON1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GAGGCTGTCC CCGGCGCCCC TGAAGCTGAG CCGGACTCCG GCACTGCTGG 1081 CCCTGGCGCT GCCCCTGGCC GCGGCGCTGG CCTTCTCCGA CGAGACCCTG GACAAAGTGC 1141 CCAAGTCAGA GGGCTACTGC AGCCGTATCC TGCGCGCCCA GGGCACGCGG CGCGAGGGCT 1201 ACACCGAGTT CAGCCTCCGC GTGGAGGGCG ACCCCGACTT CTACAAGCCG GGAACCAGCT 1261 ACCGCGTAAC ACTTTCAGCT GCTCCTCCCT CCTACTTCAG AGGATTCACA TTAATTGCCC 1321 TCAGAGAGAA CAGAGAGGGT GATAAGGAAG AAGACCATGC TGGGACCTTC CAGATCATAG 1381 ACGAAGAAGA AACTCAGTTT ATGAGCAATT GCCCTGTTGC AGTCACTGAA AGCACTCCAC 1441 GGAGGAGGAC CCGGATCCAG GTGTTTTGGA TAGCACCACC AGCGGGAACA GGCTGCGTGA 1501 TTCTGAAGGC CAGCATCGTA CAAAAACGCA TTATTTATTT TCAAGATGAG GGCTCTCTGA 1561 CCAAGAAACT TTGTGAACAA GATTCCACAT TTGATGGGGT GACTGACAAA CCCATCTTAG 1621 ACTGCTGTGC CTGCGGAACT GCCAAGTACA GACTCACATT TTATGGGAAT TGGTCCGAGA 1681 AGACACACCC AAAGGATTAC CCTCGTCGGG CCAACCACTG GTCTGCGATC ATCGGAGGAT 1741 CCCACTCCAA GAATTATGTA CTGTGGGAAT ATGGAGGATA TGCCAGCGAA GGCGTCAAAC 1801 AAGTTGCAGA ATTGGGCTCA CCCGTGAAAA TGGAGGAAGA AATTCGACAA CAGAGTGATG 1861 AGGTCCTCAC CGTCATCAAA GCCAAAGCCC AGTGGCCAGC CTGGCAGCCT CTCAACGTGA 1921 GAGCAGCACC TTCAGCTGAA TTTTCCGTGG ACAGAACGCG CCATTTAATG TCCTTCCTGA 1981 CCATGATGGG CCCTAGTCCC GACTGGAACG TAGGCTTATC TGCAGAAGAT CTGTGCACCA 2041 AGGAATGTGG CTGGGTCCAG AAGGTGGTGC AAGACCTGAT TCCCTGGGAC GCTGGCACCG 2101 ACAGCGGGGT GACCTATGAG TCACCCAACA AACCCACCAT TCCCCAGGAG AAAATCCGGC 2161 CCCTGACCAG CCTGGACCAT CCTCAGAGTC CTTTCTATGA CCCAGAGGGT GGGTCCATCA 2221 CTCAAGTAGC CAGAGTTGTC ATCGAGAGAA TCGCACGGAA GGGTGAACAA TGCAATATTG 2281 TACCTGACAA TGTCGATGAT ATTGTAGCTG ACCTGGCTCC AGAAGAGAAA GATGAAGATG 2341 ACACCCCTGA AACCTGCATC TACTCCAACT GGTCCCCATG GTCCGCCTGC AGCTCCTCCA 2401 CCTGTGACAA AGGCAAGAGG ATGCGACAGC GCATGCTGAA AGCACAGCTG GACCTCAGCG 2461 TCCCCTGCCC TGACACCCAG GACTTCCAGC CCTGCATGGG CCCTGGCTGC AGTGACGAAG 2521 ACGGCTCCAC CTGCACCATG TCCGAGTGGA TCACCTGGTC GCCCTGCAGC ATCTCCTGCG 2581 GCATGGGCAT GAGGTCCCGG GAGAGGTATG TGAAGCAGTT CCCGGAGGAC GGCTCCGTGT 2641 GCACGCTGCC CACTGAGGAA ACGGAGAAGT GCACGGTCAA CGAGGAGTGC TCTCCCAGCA 2701 GCTGCCTGAT GACCGAGTGG GGCGAGTGGG ACGAGTACAG CGCCACCTGC GGCATGGGCA 2761 TGAAGAAGCG GCACCGCATG ATCAAGATGA ACCCCGCAGA TGGCTCCATG TGCAAAGCCG 2821 AGACATCACA GGCAGAGAAG TGCATGATGC CAGAGTGCCA CACCATCCCA TGCTTGCTGT 2881 CCCCATGGTC CGAGTGGAGT GACTGCAGCG TGACCTGCGG GAAGGGCATG CGAACCCGAC 2941 AGCGGATGCT CAAGTCTCTG GCAGAACTTG GAGACTGCAA TGAGGATCTG GAGCAGGTGG 3001 AGAAGTGCAT GCTCCCTGAA TGCCCCATTG ACTGTGAGCT CACCGAGTGG TCCCAGTGGT 3061 CGGAATGTAA CAAGTCATGT GGGAAAGGCC ACGTGATTCG AACCCGGATG ATCCAAATGG 3121 AGCCTCAGTT TGGAGGTGCA CCCTGCCCAG AGACTGTGCA GCGAAAAAAG TGCCGCATCC 3181 GAAAATGCCT TCGAAATCCA TCCATCCAAA AGCTACGCTG GAGGGAGGCC CGAGAGAGCC 3241 GGCGGAGTGA GCAGCTGAAG GAAGAGTCTG AAGGGGAGCA GTTCCCAGGT TGTAGGATGC 3301 GCCCATGGAC GGCCTGGTCA GAATGCACCA AACTGTGCGG AGGTGGAATT CAGGAACGTT 3361 ACATGACTGT AAAGAAGAGA TTCAAAAGCT CCCAGTTTAC CAGCTGCAAA GACAAGAAGG 3421 AGATCAGAGC ATGCAATGTT CATCCTTGTA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 3481 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 3541 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 3601 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 3661 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 3721 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 3781 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 3841 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 3901 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 3961 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 4021 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 4081 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 4141 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 4201 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 4261 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 4321 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 4381 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 4441 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 4501 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 4561 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 4621 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 4681 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 4741 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 4801 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 4861 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 4921 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 4981 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 5041 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 5101 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 5161 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 5221 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 5281 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 5341 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 5401 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 5461 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 5521 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 5581 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 5641 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 5701 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 5761 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 5821 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 5881 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 5941 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 6001 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 6061 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 6121 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 6181 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 6241 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 6301 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 6361 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 6421 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 6481 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 6541 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 6601 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 6661 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 6721 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 6781 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 6841 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 6901 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 6961 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 7021 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 7081 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 7141 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 7201 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 7261 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 7321 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 7381 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 7441 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 7501 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 7561 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 7621 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 7681 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 7741 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 7801 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 7861 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 7921 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 7981 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 8041 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 8101 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 8161 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 8221 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 8281 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 8341 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 8401 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 8461 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 8521 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 8581 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 8641 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 8701 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 8761 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 8821 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 8881 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 8941 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T //