LOCUS       RG217978                9245 bp    DNA     circular  SYN 
DEFINITION  NLRP6 (GFP-tagged) - Human NLR family, pyrin domain containing 6 (NLRP6)
ACCESSION   NM_138329
VERSION     NM_138329.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3724..4428 
                     /label="GFP"  
     misc_feature    4475..5060 
                     /label="PolyA signal"  
     misc_feature    7152..7797 
                     /label="ColE1"  
     misc_feature    5514..6308 
                     /label="Neo"  
     misc_feature    5145..5447 
                     /label="SV40 ori"  
     misc_feature    7812..8672 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3696..3703 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3696 
                     /label="NLRP6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCAGCCA GAGGCCCCCT GCTCCAGCAC GGGGCCGCGC CTCGCGGTGG 
     1081 CCCGCGAGCT GCTCCTGGCT GCGCTGGAGG AACTGAGCCA AGAGCAGCTG AAGCGCTTCC 
     1141 GCCACAAGCT GCGCGACGTG GGCCCGGACG GACGCAGCAT CCCGTGGGGG CGGCTGGAGC 
     1201 GCGCGGACGC CGTGGACCTC GCGGAGCAGC TGGCCCAGTT CTACGGCCCG GAGCCTGCCC 
     1261 TGGAGGTGGC CCGCAAGACC CTCAAGAGGG CGGACGCGCG CGACGTGGCG GCGCAGCTCC 
     1321 AGGAGCGGCG GCTGCAGCGG CTCGGGCTCG GCTCCGGGAC GCTGCTCTCC GTGTCCGAGT 
     1381 ACAAGAAGAA GTACCGGGAG CACGTGCTGC AGCTGCACGC TCGGGTGAAG GAGAGGAACG 
     1441 CCCGCTCCGT GAAGATCACC AAGCGCTTCA CCAAGCTGCT CATCGCGCCC GAGAGCGCCG 
     1501 CCCCGGAGGA GGCGCTGGGG CCCGCGGAAG AGCCTGAGCC GGGGCGCGCG CGGCGCTCGG 
     1561 ACACGCACAC TTTCAACCGC CTCTTCCGCC GCGACGAGGA GGGCCGGCGG CCGCTGACCG 
     1621 TGGTGCTGCA GGGCCCGGCG GGCATCGGCA AGACCATGGC GGCCAAAAAG ATCCTGTACG 
     1681 ACTGGGCGGC GGGCAAGCTG TACCAGGGCC AGGTGGACTT CGCCTTCTTC ATGCCCTGCG 
     1741 GCGAGCTGCT GGAGAGGCCG GGCACGCGCA GCCTGGCTGA CCTGATCCTG GACCAGTGCC 
     1801 CCGACCGCGG CGCGCCGGTG CCGCAGATGC TGGCCCAGCC GCAGCGGCTG CTCTTCATCC 
     1861 TGGACGGCGC GGACGAGCTG CCGGCGCTGG GGGGCCCCGA GGCCGCGCCC TGCACAGACC 
     1921 CCTTCGAGGC GGCGAGCGGC GCGCGGGTGC TAGGCGGGCT GCTGAGCAAG GCGCTGCTGC 
     1981 CCACGGCCCT CCTGCTGGTG ACCACGCGCG CCGCCGCCCC CGGGAGGCTG CAGGGCCGCC 
     2041 TGTGTTCCCC GCAGTGCGCC GAGGTGCGCG GCTTCTCCGA CAAGGACAAG AAGAAGTATT 
     2101 TCTACAAGTT CTTCCGGGAT GAGAGGAGGG CCGAGCGCGC CTACCGCTTC GTGAAGGAGA 
     2161 ACGAGACGCT GTTCGCGCTG TGCTTCGTGC CCTTCGTGTG CTGGATCGTG TGCACCGTGC 
     2221 TGCGCCAGCA GCTGGAGCTC GGTCGGGACC TGTCGCGCAC GTCCAAGACC ACCACGTCAG 
     2281 TGTACCTGCT TTTCATCACC AGCGTTCTGA GCTCGGCTCC GGTAGCCGAC GGGCCCCGGT 
     2341 TGCAGGGCGA CCTGCGCAAT CTGTGCCGCC TGGCCCGCGA GGGCGTCCTC GGACGCAGGG 
     2401 CGCAGTTTGC CGAGAAGGAA CTGGAGCAAC TGGAGCTTCG TGGCTCCAAA GTGCAGACGC 
     2461 TGTTTCTCAG CAAAAAGGAG CTGCCGGGCG TGCTGGAGAC AGAGGTCACC TACCAGTTCA 
     2521 TCGACCAGAG CTTCCAGGAG TTCCTCGCGG CACTGTCCTA CCTGCTGGAG GACGGCGGGG 
     2581 TGCCCAGGAC CGCGGCTGGC GGCGTTGGGA CACTCCTGCG TGGGGACGCC CAGCCGCACA 
     2641 GCCACTTGGT GCTCACCACG CGCTTCCTCT TCGGACTGCT GAGCGCGGAG CGGATGCGCG 
     2701 ACATCGAGCG CCACTTCGGC TGCATGGTTT CAGAGCGTGT GAAGCAGGAG GCCCTGCGGT 
     2761 GGGTGCAGGG ACAGGGACAG GGCTGCCCCG GAGTGGCACC AGAGGTGACC GAGGGGGCCA 
     2821 AAGGGCTCGA GGACACCGAA GAGCCAGAGG AGGAGGAGGA GGGAGAGGAG CCCAACTACC 
     2881 CACTGGAGTT GCTGTACTGC CTGTACGAGA CGCAGGAGGA CGCGTTTGTG CGCCAAGCCC 
     2941 TGTGCCGGTT CCCGGAGCTG GCGCTGCAGC GAGTGCGCTT CTGCCGCATG GACGTGGCTG 
     3001 TTCTGAGCTA CTGCGTGAGG TGCTGCCCTG CTGGACAGGC ACTGCGGCTG ATCAGCTGCA 
     3061 GATTGGTTGC TGCGCAGGAG AAGAAGAAGA AGAGCCTGGG GAAGCGGCTC CAGGCCAGCC 
     3121 TGGGTGGCGG CAGCAGTTCT CAAGGCACCA CAAAACAACT GCCAGCCTCC CTTCTTCATC 
     3181 CACTCTTTCA GGCAATGACT GACCCACTGT GCCATCTGAG CAGCCTCACG CTGTCCCACT 
     3241 GCAAACTCCC TGACGCGGTC TGCCGAGACC TTTCTGAGGC CCTGAGGGCA GCCCCCGCAC 
     3301 TGACGGAGCT GGGCCTCCTC CACAACAGGC TCAGTGAGGC GGGACTGCGT ATGCTGAGTG 
     3361 AGGGCCTAGC CTGGCCGCAG TGCAGGGTGC AGACGGTCAG GGTACAGCTG CCTGACCCCC 
     3421 AGCGAGGGCT CCAGTACCTG GTGGGTATGC TTCGGCAGAG CCCTGCCCTG ACCACCCTGG 
     3481 ATCTCAGCGG CTGCCAACTG CCCGCCCCCA TGGTGACCTA CCTGTGTGCA GTCCTGCAGC 
     3541 ACCAGGGATG CGGCCTGCAG ACCCTCAGTC TGGCCTCTGT GGAGCTGAGC GAGCAGTCAC 
     3601 TACAGGAGCT TCAGGCTGTG AAGAGAGCAA AGCCGGATCT GGTCATCACA CACCCAGCGC 
     3661 TGGACGGCCA CCCACAACCT CCCAAGGAAC TCATCTCGAC CTTCAGCGGA CCGACGCGTA 
     3721 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3781 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3841 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     3901 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     3961 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4021 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4081 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4141 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4201 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4261 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4321 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4381 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4441 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4501 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4561 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4621 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4681 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4741 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4801 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4861 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4921 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4981 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5041 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5101 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5161 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5221 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5281 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5341 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5401 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5461 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5521 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5581 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5641 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5701 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5761 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5821 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     5881 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     5941 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6001 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6061 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6121 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6181 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6241 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6301 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6361 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6421 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6481 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6541 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6601 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6661 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6721 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6781 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6841 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     6901 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     6961 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7021 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7081 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7141 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7201 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7261 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7321 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7381 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7441 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7501 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7561 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7621 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7681 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7741 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7801 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7861 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     7921 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     7981 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8041 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8101 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8161 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8221 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8281 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8341 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8401 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8461 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8521 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8581 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8641 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8701 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8761 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8821 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     8881 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     8941 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9001 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9061 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9121 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9181 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9241 ATTTT
//