LOCUS       RG217684                9461 bp    DNA     circular  SYN 
DEFINITION  PTK2B (GFP-tagged) - Human PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 4
ACCESSION   NM_173175
VERSION     NM_173175.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3942..4646 
                     /label="GFP"  
     misc_feature    4693..5278 
                     /label="PolyA signal"  
     misc_feature    7370..8015 
                     /label="ColE1"  
     misc_feature    5732..6526 
                     /label="Neo"  
     misc_feature    5363..5665 
                     /label="SV40 ori"  
     misc_feature    8030..8890 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3921..3927 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3921 
                     /label="PTK2B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGGGGTG TCCGAGCCCC TGAGTCGAGT AAAGTTGGGC ACGTTACGCC 
     1081 GGCCTGAAGG CCCTGCAGAG CCCATGGTGG TGGTACCAGT AGATGTGGAA AAGGAGGACG 
     1141 TGCGTATCCT CAAGGTCTGC TTCTATAGCA ACAGCTTCAA TCCTGGGAAA AACTTCAAAC 
     1201 TGGTCAAATG CACTGTCCAG ACGGAGATCC GGGAGATCAT CACCTCCATC CTGCTGAGCG 
     1261 GGCGGATCGG GCCCAACATC CGGTTGGCTG AGTGCTATGG GCTGAGGCTG AAGCACATGA 
     1321 AGTCCGATGA GATCCACTGG CTGCACCCAC AGATGACGGT GGGTGAGGTG CAGGACAAGT 
     1381 ATGAGTGTCT GCACGTGGAA GCCGAGTGGA GGTATGACCT TCAAATCCGC TACTTGCCAG 
     1441 AAGACTTCAT GGAGAGCCTG AAGGAGGACA GGACCACGCT GCTCTATTTT TACCAACAGC 
     1501 TCCGGAACGA CTACATGCAG CGCTACGCCA GCAAGGTCAG CGAGGGCATG GCCCTGCAGC 
     1561 TGGGCTGCCT GGAGCTCAGG CGGTTCTTCA AGGATATGCC CCACAATGCA CTTGACAAGA 
     1621 AGTCCAACTT CGAGCTCCTA GAAAAGGAAG TGGGGCTGGA CTTGTTTTTC CCAAAGCAGA 
     1681 TGCAGGAGAA CTTAAAGCCC AAACAGTTCC GGAAGATGAT CCAGCAGACC TTCCAGCAGT 
     1741 ACGCCTCGCT CAGGGAGGAG GAGTGCGTCA TGAAGTTCTT CAACACTCTC GCCGGCTTCG 
     1801 CCAACATCGA CCAGGAGACC TACCGCTGTG AACTCATTCA AGGATGGAAC ATTACTGTGG 
     1861 ACCTGGTCAT TGGCCCTAAA GGGATCCGCC AGCTGACTAG TCAGGACGCA AAGCCCACCT 
     1921 GCCTGGCCGA GTTCAAGCAG ATCAGGTCCA TCAGGTGCCT CCCGCTGGAG GAGGGCCAGG 
     1981 CAGTACTTCA GCTGGGCATT GAAGGTGCCC CCCAGGCCTT GTCCATCAAA ACCTCATCCC 
     2041 TAGCAGAGGC TGAGAACATG GCTGACCTCA TAGACGGCTA CTGCCGGCTG CAGGGTGAGC 
     2101 ACCAAGGCTC TCTCATCATC CATCCTAGGA AAGATGGTGA GAAGCGGAAC AGCCTGCCCC 
     2161 AGATCCCCAT GCTAAACCTG GAGGCCCGGC GGTCCCACCT CTCAGAGAGC TGCAGCATAG 
     2221 AGTCAGACAT CTACGCAGAG ATTCCCGACG AAACCCTGCG AAGGCCCGGA GGTCCACAGT 
     2281 ATGGCATTGC CCGTGAAGAT GTGGTCCTGA ATCGTATTCT TGGGGAAGGC TTTTTTGGGG 
     2341 AGGTCTATGA AGGTGTCTAC ACAAATCACA AAGGGGAGAA AATCAATGTA GCTGTCAAGA 
     2401 CCTGCAAGAA AGACTGCACT CTGGACAACA AGGAGAAGTT CATGAGCGAG GCAGTGATCA 
     2461 TGAAGAACCT CGACCACCCG CACATCGTGA AGCTGATCGG CATCATTGAA GAGGAGCCCA 
     2521 CCTGGATCAT CATGGAATTG TATCCCTATG GGGAGCTGGG CCACTACCTG GAGCGGAACA 
     2581 AGAACTCCCT GAAGGTGCTC ACCCTCGTGC TGTACTCACT GCAGATATGC AAAGCCATGG 
     2641 CCTACCTGGA GAGCATCAAC TGCGTGCACA GGGACATTGC TGTCCGGAAC ATCCTGGTGG 
     2701 CCTCCCCTGA GTGTGTGAAG CTGGGGGACT TTGGTCTTTC CCGGTACATT GAGGACGAGG 
     2761 ACTATTACAA AGCCTCTGTG ACTCGTCTCC CCATCAAATG GATGTCCCCA GAGTCCATTA 
     2821 ACTTCCGACG CTTCACGACA GCCAGTGACG TCTGGATGTT CGCCGTGTGC ATGTGGGAGA 
     2881 TCCTGAGCTT TGGGAAGCAG CCCTTCTTCT GGCTGGAGAA CAAGGATGTC ATCGGGGTGC 
     2941 TGGAGAAAGG AGACCGGCTG CCCAAGCCTG ATCTCTGTCC ACCGGTCCTT TATACCCTCA 
     3001 TGACCCGCTG CTGGGACTAC GACCCCAGTG ACCGGCCCCG CTTCACCGAG CTGGTGTGCA 
     3061 GCCTCAGTGA CGTTTATCAG ATGGAGAAGG ACATTGCCAT GGAGCAAGAG AGGAATGCTC 
     3121 GCTACCGAAC CCCCAAAATC TTGGAGCCCA CAGCCTTCCA GGAACCCCCA CCCAAGCCCA 
     3181 GCCGACCTAA GTACAGACCC CCTCCGCAAA CCAACCTCCT GGCTCCAAAG CTGCAGTTCC 
     3241 AGGAGGAGGA CTTCATCCAA CCCAGCAGCC GAGAAGAGGC CCAGCAGCTG TGGGAGGCTG 
     3301 AAAAGGTCAA AATGCGGCAA ATCCTGGACA AACAGCAGAA GCAGATGGTG GAGGACTACC 
     3361 AGTGGCTCAG GCAGGAGGAG AAGTCCCTGG ACCCCATGGT TTATATGAAT GATAAGTCCC 
     3421 CATTGACGCC AGAGAAGGAG GTCGGCTACC TGGAGTTCAC AGGGCCCCCA CAGAAGCCCC 
     3481 CGAGGCTGGG CGCACAGTCC ATCCAGCCCA CAGCTAACCT GGACCGGACT GATGACCTGG 
     3541 TGTACCTCAA TGTCATGGAG CTGGTGCGGG CCGTGCTGGA GCTCAAGAAT GAGCTCTGTC 
     3601 AGCTGCCCCC CGAGGGCTAC GTGGTGGTGG TGAAGAATGT GGGGCTGACC CTGCGGAAGC 
     3661 TCATCGGGAG CGTGGATGAT CTCCTGCCTT CCTTGCCGTC ATCTTCACGG ACAGAGATCG 
     3721 AGGGCACCCA GAAACTGCTC AACAAAGACC TGGCAGAGCT CATCAACAAG ATGCGGCTGG 
     3781 CACAGCAGAA CGCCGTGACC TCCCTAAGTG AGGAGTGCAA GAGGCAGATG CTGACGGCTT 
     3841 CACACACCCT GGCTGTGGAC GCCAAGAACC TGCTCGACGC TGTGGACCAG GCCAAGGTTC 
     3901 TGGCCAATCT GGCCCACCCA CCTGCAGAGA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3961 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     4021 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     4081 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     4141 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     4201 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     4261 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4321 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4381 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4441 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4501 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4561 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4621 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4681 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4741 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4801 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4861 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4921 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4981 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5041 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5101 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5161 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5221 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5281 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5341 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5401 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5461 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5521 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5581 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5641 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5701 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5761 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5821 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5881 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5941 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     6001 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     6061 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     6121 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     6181 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     6241 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6301 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6361 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6421 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6481 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6541 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6601 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6661 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6721 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6781 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6841 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6901 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6961 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     7021 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     7081 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     7141 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     7201 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     7261 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7321 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7381 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7441 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7501 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7561 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7621 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7681 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7741 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7801 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7861 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7921 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7981 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     8041 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     8101 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     8161 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     8221 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     8281 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8341 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8401 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8461 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8521 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8581 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8641 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8701 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8761 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8821 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8881 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8941 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     9001 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     9061 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     9121 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     9181 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     9241 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9301 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9361 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9421 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//