LOCUS       RG217615                9335 bp    DNA     circular  SYN 
DEFINITION  DDX58 (GFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58)
ACCESSION   NM_014314
VERSION     NM_014314.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3816..4520 
                     /label="GFP"  
     misc_feature    4567..5152 
                     /label="PolyA signal"  
     misc_feature    7244..7889 
                     /label="ColE1"  
     misc_feature    5606..6400 
                     /label="Neo"  
     misc_feature    5237..5539 
                     /label="SV40 ori"  
     misc_feature    7904..8764 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3795..3801 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3795 
                     /label="DDX58"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCACCGAG CAGCGACGCA GCCTGCAAGC CTTCCAGGAT TATATCCGGA 
     1081 AGACCCTGGA CCCTACCTAC ATCCTGAGCT ACATGGCCCC CTGGTTTAGG GAGGAAGAGG 
     1141 TGCAGTATAT TCAGGCTGAG AAAAACAACA AGGGCCCAAT GGAGGCTGCC ACACTTTTTC 
     1201 TCAAGTTCCT GTTGGAGCTC CAGGAGGAAG GCTGGTTCCG TGGCTTTTTG GATGCCCTAG 
     1261 ACCATGCAGG TTATTCTGGA CTTTATGAAG CCATTGAAAG TTGGGATTTC AAAAAAATTG 
     1321 AAAAGTTGGA GGAGTATAGA TTACTTTTAA AACGTTTACA ACCAGAATTT AAAACCAGAA 
     1381 TTATCCCAAC CGATATCATT TCTGATCTGT CTGAATGTTT AATTAATCAG GAATGTGAAG 
     1441 AAATTCTACA GATTTGCTCT ACTAAGGGGA TGATGGCAGG TGCAGAGAAA TTGGTGGAAT 
     1501 GCCTTCTCAG ATCAGACAAG GAAAACTGGC CCAAAACTTT GAAACTTGCT TTGGAGAAAG 
     1561 AAAGGAACAA GTTCAGTGAA CTGTGGATTG TAGAGAAAGG TATAAAAGAT GTTGAAACAG 
     1621 AAGATCTTGA GGATAAGATG GAAACTTCTG ACATACAGAT TTTCTACCAA GAAGATCCAG 
     1681 AATGCCAGAA TCTTAGTGAG AATTCATGTC CACCTTCAGA AGTGTCTGAT ACAAACTTGT 
     1741 ACAGCCCATT TAAACCAAGA AATTACCAAT TAGAGCTTGC TTTGCCTGCT ATGAAAGGAA 
     1801 AAAACACAAT AATATGTGCT CCTACAGGTT GTGGAAAAAC CTTTGTTTCA CTGCTTATAT 
     1861 GTGAACATCA TCTTAAAAAA TTCCCACAAG GACAAAAGGG GAAAGTTGTC TTTTTTGCGA 
     1921 ATCAGATCCC AGTGTATGAA CAGCAGAAAT CTGTATTCTC AAAATACTTT GAAAGACATG 
     1981 GGTATAGAGT TACAGGCATT TCTGGAGCAA CAGCTGAGAA TGTCCCAGTG GAACAGATTG 
     2041 TTGAGAACAA TGACATCATC ATTTTAACTC CACAGATTCT TGTGAACAAC CTTAAAAAGG 
     2101 GAACGATTCC ATCACTATCC ATCTTTACTT TGATGATATT TGATGAATGC CACAACACTA 
     2161 GTAAACAACA CCCGTACAAT ATGATCATGT TTAATTATCT AGATCAGAAA CTTGGAGGAT 
     2221 CTTCAGGCCC ACTGCCCCAG GTCATTGGGC TGACTGCCTC GGTTGGTGTT GGGGATGCCA 
     2281 AAAACACAGA TGAAGCCTTG GATTATATCT GCAAGCTGTG TGCTTCTCTT GATGCGTCAG 
     2341 TGATAGCAAC AGTCAAACAC AATCTGGAGG AACTGGAGCA AGTTGTTTAT AAGCCCCAGA 
     2401 AGTTTTTCAG GAAAGTGGAA TCACGGATTA GCGACAAATT TAAATACATC ATAGCTCAGC 
     2461 TGATGAGGGA CACAGAGAGT CTGGCAAAGA GAATCTGCAA AGACCTCGAA AACTTATCTC 
     2521 AAATTCAAAA TAGGGAATTT GGAACACAGA AATATGAACA ATGGATTGTT ACAGTTCAGA 
     2581 AAGCATGCAT GGTGTTCCAG ATGCCAGACA AAGATGAAGA GAGCAGGATT TGTAAAGCCC 
     2641 TGTTTTTATA CACTTCACAT TTGCGGAAAT ATAATGATGC CCTCATTATC AGTGAGCATG 
     2701 CACGAATGAA AGATGCTCTG GATTACTTGA AAGACTTCTT CAGCAATGTC CGAGCAGCAG 
     2761 GATTCGATGA GATTGAGCAA GATCTTACTC AGAGATTTGA AGAAAAGCTG CAGGAACTAG 
     2821 AAAGTGTTTC CAGGGATCCC AGCAATGAGA ATCCTAAACT TGAAGACCTC TGCTTCATCT 
     2881 TACAAGAAGA GTACCACTTA AACCCAGAGA CAATAACAAT TCTCTTTGTG AAAACCAGAG 
     2941 CACTTGTGGA CGCTTTAAAA AATTGGATTG AAGGAAATCC TAAACTCAGT TTTCTAAAAC 
     3001 CTGGCATATT GACTGGACGT GGCAAAACAA ATCAGAACAC AGGAATGACC CTCCCGGCAC 
     3061 AGAAGTGTAT ATTGGATGCA TTCAAAGCCA GTGGAGATCA CAATATTCTG ATTGCCACCT 
     3121 CAGTTGCTGA TGAAGGCATT GACATTGCAC AGTGCAATCT TGTCATCCTT TATGAGTATG 
     3181 TGGGCAATGT CATCAAAATG ATCCAAACCA GAGGCAGAGG AAGAGCAAGA GGTAGCAAGT 
     3241 GCTTCCTTCT GACTAGTAAT GCTGGTGTAA TTGAAAAAGA ACAAATAAAC ATGTACAAAG 
     3301 AAAAAATGAT GAATGACTCT ATTTTACGCC TTCAGACATG GGACGAAGCA GTATTTAGGG 
     3361 AAAAGATTCT GCACATACAG ACTCATGAAA AATTCATCAG AGATAGTCAA GAAAAACCAA 
     3421 AACCTGTACC TGATAAGGAA AATAAAAAAC TGCTCTGCAG AAAGTGCAAA GCCTTGGCAT 
     3481 GTTACACAGC TGACGTAAGA GTGATAGAGG AATGCCATTA CACTGTGCTT GGAGATGCTT 
     3541 TTAAGGAATG CTTTGTGAGT AGACCACATC CCAAGCCAAA GCAGTTTTCA AGTTTTGAAA 
     3601 AAAGAGCAAA GATATTCTGT GCCCGACAGA ACTGCAGCCA TGACTGGGGA ATCCATGTGA 
     3661 AGTACAAGAC ATTTGAGATT CCAGTTATAA AAATTGAAAG TTTTGTGGTG GAGGATATTG 
     3721 CAACTGGAGT TCAGACACTG TACTCGAAGT GGAAGGACTT TCATTTTGAG AAGATACCAT 
     3781 TTGATCCAGC AGAAATGTCC AAAACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3841 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     3901 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     3961 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     4021 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     4081 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     4141 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     4201 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     4261 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4321 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4381 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4441 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4501 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     5221 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5281 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5341 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5401 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5461 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5521 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5581 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5641 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5701 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5761 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5821 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     5881 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     5941 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     6001 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     6061 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     6121 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     6181 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     6241 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6301 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6361 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6421 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6481 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6541 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6721 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6781 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6841 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     6901 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     6961 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     7021 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     7081 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     7141 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     7201 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     7261 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7321 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7381 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7441 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7501 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7561 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7621 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7681 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7741 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7801 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7861 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     7921 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     7981 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     8041 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     8101 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     8161 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     8221 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     8281 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8341 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8401 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8461 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8521 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8581 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8641 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8701 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8761 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8821 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     8881 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     8941 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     9001 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     9061 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     9121 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     9181 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     9241 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9301 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//