LOCUS       RG217551                9179 bp    DNA     circular  SYN 
DEFINITION  NDST3 (GFP-tagged) - Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3)
ACCESSION   NM_004784
VERSION     NM_004784.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3660..4364 
                     /label="GFP"  
     misc_feature    4411..4996 
                     /label="PolyA signal"  
     misc_feature    7088..7733 
                     /label="ColE1"  
     misc_feature    5450..6244 
                     /label="Neo"  
     misc_feature    5081..5383 
                     /label="SV40 ori"  
     misc_feature    7748..8608 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3639..3645 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3639 
                     /label="NDST3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTTTATC ATGAAGCTTC ACAGACACTT TCAAAGAACA GTCATTCTGC 
     1081 TTGCCACTTT TTGTATGGTG AGCATTATCA TTTCTGCTTA CTACCTGTAC AGTGGCTACA 
     1141 AACAGGAAAA TGAACTCTCT GAGACGGCTT CAGAAGTTGA CTGTGGCGAC CTCCAACACC 
     1201 TACCATATCA ACTAATGGAA GTGAAAGCAA TGAAGCTTTT TGATGCCTCA AGGACAGACC 
     1261 CCACAGTCCT AGTATTTGTA GAGAGCCAGT ACTCATCTCT TGGTCAAGAC ATCATTATGA 
     1321 TTCTAGAATC AAGTAGATTC CAGTATCACA TTGAAATTGC CCCTGGAAAG GGAGATCTCC 
     1381 CAGTGCTTAT AGACAAAATG AAAGGCAAAT ACATTCTCAT TATTTATGAG AATATTTTAA 
     1441 AGTATATAAA TATGGATTCC TGGAATCGAA GCCTTCTAGA TAAATACTGT GTAGAATATG 
     1501 GTGTGGGTGT CATTGGATTC CACAAAACTA GTGAGAAGAG TGTACAGAGC TTTCAGTTAA 
     1561 AAGGTTTCCC TTTTTCCATA TATGGAAATC TTGCAGTAAA AGATTGTTGT ATTAATCCTC 
     1621 ATTCTCCATT GATTCGTGTG ACCAAATCTT CCAAGCTTGA AAAAGGTTCT TTACCTGGAA 
     1681 CTGACTGGAC AGTTTTTCAG ATTAATCATT CAGCCTATCA ACCAGTAATA TTTGCCAAAG 
     1741 TAAAGACCCC AGAAAACCTT TCTCCTTCCA TCTCTAAAGG TGCTTTTTAT GCCACTATTA 
     1801 TACATGACCT GGGGCTTCAT GATGGAATTC AAAGGGTTCT TTTTGGCAAC AACTTGAACT 
     1861 TTTGGCTGCA CAAGCTCATC TTCATAGATG CCATCTCCTT CTTATCAGGG AAGAGGCTGA 
     1921 CATTGTCCTT GGACAGGTAC ATTCTTGTGG ATATTGATGA TATATTTGTG GGAAAAGAGG 
     1981 GAACAAGAAT GAACACCAAT GATGTAAAGG CCCTGCTTGA TACTCAGAAT CTTTTGCGTG 
     2041 CACAAATCAC AAATTTTACA TTCAACCTGG GATTTTCAGG GAAATTTTAC CATACAGGAA 
     2101 CTGAAGAGGA AGATGAAGGA GATGACTGTC TGTTGGGGTC TGTGGATGAG TTCTGGTGTT 
     2161 TTCCTCACAT GTGGAGCCAT ATGCAGCCCC ACCTCTTCCA CAATGAGTCA TCTTTGGTGG 
     2221 AGCAGATGAT TCTCAACAAA AAATTTGCCT TAGAGCACGG CATTCCAACG GACATGGGCT 
     2281 ACGCTGTGGC CCCTCACCAT TCGGGCGTCT ACCCTGTACA TGTTCAGCTT TACGAGGCCT 
     2341 GGAAGAAGGT CTGGAATATT AAAATCACCA GCACTGAAGA ATATCCACAT CTGAAGCCAG 
     2401 CTAGATACCG GAGGGGTTTT ATCCACAAAA ACATCATGGT TCTCCCAAGA CAAACCTGTG 
     2461 GGCTTTTCAC TCACACCATT TTCTACAAAG AATATCCAGG GGGTCCTAAA GAGCTGGATA 
     2521 AGAGTATCCA AGGAGGAGAA CTTTTCTTCA CTGTCGTCCT CAACCCTATC AGCATTTTCA 
     2581 TGACCCATTT GTCCAACTAT GGGAATGACC GACTGGGATT ATATACATTT GTTAATCTGG 
     2641 CCAACTTTGT GAAGAGCTGG ACCAACCTGC GACTTCAGAC TCTGCCTCCA GTACAACTGG 
     2701 CCCACAAGTA TTTTGAGCTG TTTCCTGATC AGAAAGACCC TCTCTGGCAG AATCCTTGCG 
     2761 ATGACAAACG CCACAGAGAC ATTTGGTCTA AAGAAAAAAC TTGTGATCGC TTACCAAAAT 
     2821 TCTTGGTAAT AGGACCCCAG AAAACTGGTA CCACTGCTTT GTATTTGTTC CTGGTTATGC 
     2881 ATCCTTCCAT CCTTAGTAAC TCCCCCAGCC CAAAAACCTT TGAGGAGGTA CAGTTCTTTA 
     2941 ATAGAAATAA CTACCACAGG GGGATTGATT GGTATATGGA TTTCTTCCCA GTCCCATCTA 
     3001 ATGTCACTAC CGACTTTTTG TTTGAGAAGA GTGCCAATTA CTTCCACTCA GAGGAAGCCC 
     3061 CTAAAAGAGC TGCTTCTCTG GTTCCCAAAG CCAAGATTAT CACCATTCTC ATTGACCCTT 
     3121 CAGACCGAGC ATACTCCTGG TACCAGCATC AGCGATCACA TGAAGAYCCT GCAGCTCTGA 
     3181 AGTTTAGCTT CTACGAAGTG ATCTCAGCAG GGCCCCGTGC ACCCTCGGAG CTCAGAGCCT 
     3241 TGCAGAAGAG ATGTTTGGTC CCGGGGTGGT ATGCCAGCCA CATCGAGAGA TGGCTTGTTT 
     3301 ATTTCCCCCC ATTTCAGTTG CTAATTATTG ATGGGCAACA ACTAAGAACT GATCCTGCTA 
     3361 CAGTGATGGA TGAAGTACAG AAGTTTCTAG GAGTCTTGCC TCATTATAAT TACTCAGAAG 
     3421 CTTTAACGTT TGATTCTCAT AAAGGTTTCT GGTGTCAGTT ACTGGAAGAA GGTAAAACAA 
     3481 AATGCCTTGG AAAGAGCAAA GGAAGAAAAT ACCCTCCAAT GGATTCTGAT AGCAGGACAT 
     3541 TTCTGTCAAG CTACTATCGA GATCACAACG TGGAACTCTC AAAGCTGCTG CACAAACTGG 
     3601 GTCAGCCTCT GCCATCCTGG CTGAGACAGG AGCTGCAGAA AGTAAGAACG CGTACGCGGC 
     3661 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3721 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     3781 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     3841 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     3901 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     3961 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     4021 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4081 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4141 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4201 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4261 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4321 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4381 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4441 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4501 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4561 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4621 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4681 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4741 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4801 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4861 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4921 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4981 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5041 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5101 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5161 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5221 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5281 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5341 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5401 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5461 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5521 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5581 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5641 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5701 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     5761 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     5821 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     5881 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     5941 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     6001 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6061 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6121 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6181 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6241 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6301 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6361 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6421 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6481 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6541 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6601 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6661 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6721 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     6781 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     6841 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     6901 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     6961 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     7021 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7081 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7141 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7201 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7261 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7321 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7381 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7441 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7501 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7561 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7621 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7681 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7741 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     7801 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     7861 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     7921 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     7981 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     8041 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8101 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8161 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8221 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8281 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8341 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8401 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8461 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8521 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8581 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8641 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8701 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     8761 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     8821 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     8881 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     8941 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     9001 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9061 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9121 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//