LOCUS       RG217220                8693 bp    DNA     circular  SYN 
DEFINITION  KHSRP (tGFP-tagged) - Human KH-type splicing regulatory protein (KHSRP)
ACCESSION   NM_003685
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3174..3878 
                     /label="GFP"  
     misc_feature    3925..4510 
                     /label="PolyA signal"  
     misc_feature    6602..7247 
                     /label="ColE1"  
     misc_feature    4964..5758 
                     /label="Neo"  
     misc_feature    4595..4897 
                     /label="SV40 ori"  
     misc_feature    7262..8122 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3153..3159 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3153 
                     /label="KHSRP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGACTAC AGCACGGGAG GACCCCCGCC CGGGCCGCCG CCGCCCGCCG 
     1081 GCGGGGGCGG GGGAGCCGGA GGCGCCGGGG GAGGCCCTCC GCCGGGCCCG CCAGGCGCGG 
     1141 GGGACCGGGG CGGCGGCGGT CCCTGCGGCG GCGGCCCGGG CGGGGGGTCG GCCGGGGGCC 
     1201 CCTCTCAGCC ACCCGGCGGA GGCGGCCCGG GAATCCGCAA GGACGCTTTC GCCGACGCCG 
     1261 TGCAGCGGGC CCGCCAGATT GCAGCCAAAA TTGGAGGCGA TGCTGCCACG ACAGTGAATA 
     1321 ACAGCACTCC TGATTTTGGT TTTGGGGGCC AAAAGAGACA GTTGGAAGAT GGAGATCAAC 
     1381 CGGAGAGCAA GAAGCTGGCT TCCCAGGGAG ACTCAATCAG TTCTCAACTT GGACCCATCC 
     1441 ATCCTCCCCC AAGGACTTCA ATGACAGAAG AGTACAGGGT CCCAGACGGC ATGGTGGGCC 
     1501 TGATCATTGG CAGAGGAGGT GAACAAATTA ACAAAATCCA ACAGGATTCA GGCTGCAAAG 
     1561 TACAGATTTC TCCAGACAGC GGTGGCCTAC CCGAGCGCAG TGTGTCCTTG ACAGGAGCCC 
     1621 CAGAATCTGT CCAGAAAGCC AAGATGATGC TGGATGACAT TGTGTCTCGG GGTCGTGGGG 
     1681 GCCCCCCAGG ACAGTTCCAC GACAACGCCA ACGGGGGCCA GAACGGCACC GTGCAGGAGA 
     1741 TCATGATCCC CGCGGGCAAG GCCGGCCTGG TCATTGGCAA GGGCGGGGAG ACCATTAAGC 
     1801 AGCTGCAGGA ACGCGCTGGA GTGAAGATGA TCTTAATTCA GGACGGATCT CAGAATACGA 
     1861 ATGTGGACAA ACCTCTCCGC ATCATTGGGG ATCCTTACAA AGTGCAGCAA GCCTGTGAGA 
     1921 TGGTGATGGA CATCCTCCGG GAACGTGACC AAGGCGGCTT TGGGGACCGG AATGAGTACG 
     1981 GATCTCGGAT TGGCGGAGGC ATCGATGTGC CAGTGCCCAG GCATTCTGTT GGCGTGGTCA 
     2041 TTGGCCGGAG TGGAGAGATG ATCAAGAAGA TCCAGAATGA TGCTGGCGTG CGGATACAGT 
     2101 TCAAGCAAGA TGACGGGACA GGGCCCGAGA AGATTGCTCA TATAATGGGG CCCCCAGACA 
     2161 GGTGCGAGCA CGCAGCCCGG ATCATCAACG ACCTCCTCCA GAGCCTCAGG AGTGGTCCCC 
     2221 CAGGTCCTCC AGGGGGTCCA GGCATGCCCC CGGGGGGCCG AGGCCGAGGA AGAGGCCAAG 
     2281 GCAATTGGGG TCCCCCTGGC GGGGAGATGA CCTTCTCCAT CCCCACTCAC AAGTGTGGGC 
     2341 TGGTCATCGG CCGAGGTGGC GAGAATGTGA AAGCCATAAA CCAGCAGACG GGAGCCTTCG 
     2401 TAGAGATCTC CCGGCAGCTG CCACCCAACG GGGACCCCAA CTTCAAGTTG TTCATCATCC 
     2461 GGGGTTCACC CCAGCAGATT GACCACGCCA AGCAGCTTAT CGAGGAAAAG ATCGAGGGTC 
     2521 CTCTCTGCCC AGTTGGACCA GGCCCAGGTG GCCCAGGCCC TGCTGGCCCA ATGGGGCCCT 
     2581 TCAATCCTGG GCCCTTCAAC CAGGGGCCAC CCGGGGCTCC CCCACATGCC GGGGGGCCCC 
     2641 CTCCTCACCA GTACCCACCC CAGGGCTGGG GCAATACCTA CCCCCAGTGG CAGCCGCCTG 
     2701 CTCCTCATGA CCCAAGCAAA GCAGCTGCAG CGGCCGCGGA CCCCAACGCC GCGTGGGCCG 
     2761 CCTACTACTC ACACTACTAC CAGCAGCCCC CGGGCCCCGT CCCCGGCCCC GCACCGGCCC 
     2821 CTGCGGCCCC ACCGGCTCAG GGTGAGCCCC CTCAGCCCCC ACCCACCGGC CAGTCGGACT 
     2881 ACACTAAGGC CTGGGAAGAG TATTACAAAA AGATCGGCCA GCAGCCCCAG CAGCCCGGAG 
     2941 CGCCCCCACA GCAGGACTAC ACGAAGGCTT GGGAGGAGTA CTACAAGAAG CAAGCGCAAG 
     3001 TGGCCACCGG AGGGGGTCCA GGAGCTCCCC CAGGCTCCCA GCCAGACTAC AGTGCCGCCT 
     3061 GGGCGGAATA TTACAGACAG CAGGCCGCTT ACTACGGACA GACCCCAGGT CCTGGCGGCC 
     3121 CCCAGCCGCC GCCCACGCAG CAGGGACAGC AGCAGGCTCA AACGCGTACG CGGCCGCTCG 
     3181 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3241 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     3301 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     3361 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     3421 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     3481 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     3541 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     3601 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     3661 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     3721 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     3781 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     3841 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     3901 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3961 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4021 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4081 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4141 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4201 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4261 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4321 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4381 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4441 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4501 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4561 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     4621 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     4681 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     4741 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     4801 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     4861 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     4921 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     4981 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5041 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5101 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5161 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5221 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     5281 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     5341 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     5401 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     5461 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     5521 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     5581 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     5641 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     5701 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     5761 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     5821 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     5881 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     5941 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6001 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6061 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6121 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6181 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6241 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     6301 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     6361 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     6421 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     6481 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     6541 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     6601 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     6661 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     6721 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     6781 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     6841 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     6901 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     6961 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7021 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7081 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7141 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7201 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7261 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     7321 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     7381 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     7441 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     7501 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     7561 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     7621 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     7681 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     7741 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     7801 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     7861 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     7921 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     7981 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8041 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8101 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8161 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8221 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     8281 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     8341 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     8401 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     8461 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     8521 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     8581 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     8641 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//